Simultaneous determination of tryptophan, kynurenine and 5-hydroxytryptamine by HPLC: Application in uremic patients undergoing hemodialysis

Zhen, Qianna; Xu, Bo; Ma, Li; Tang, Gang; Tang, Xiufang; Ding, Min

doi:10.1016/j.clinbiochem.2010.10.011

Cited by 55 publications

(32 citation statements)

References 31 publications

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“…Zhen et al and Koening et al used a 5-μm C18 column and adjusted the wavelength of the ultraviolet detector during detection (12,13). In the current study, a 4.5-μm C18 column was used.…”

Section: Discussionmentioning

confidence: 99%

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A modified HPLC method improves the simultaneous determination of plasma kynurenine and tryptophan concentrations in patients following maintenance hemodialysis

Xiao

Chen

Liang

et al. 2014

Experimental and Therapeutic Medicine

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The ratio between plasma kynurenine (Kyn) and tryptophan (Trp) serves as a marker of indoleamine 2,3-dioxygenase, a critical immunomodulatory molecule. Simultaneous detection of the two markers may be performed using high-pressure liquid chromatography (HPLC). However, for uremic patients, the conventional detection method may be affected by a range of accumulated toxins. The current study aimed to establish a method for the simultaneous measurement of Kyn and Trp in patients following maintenance hemodialysis via HPLC-ultraviolet detection. The procedure involved the use of a SinoChrom ODS-BP C18 column (4.6×150 mm; inner diameter, 4.5 μm) and a mobile phase of 15 mmol/l sodium acetate acetic acid solution (containing 5% acetonitrile, pH 4.8). The modified method was verified using plasma samples from 10 healthy controls and 91 maintenance hemodialysis patients. The results demonstrated that the modified method was successful in simultaneously detecting the concentrations of Trp and Kyn in the healthy controls and maintenance hemodialysis patients. The method is simple, fast, accurate and suitable for clinical and research purposes in maintenance hemodialysis patients.

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“…Zhen et al and Koening et al used a 5-μm C18 column and adjusted the wavelength of the ultraviolet detector during detection (12,13). In the current study, a 4.5-μm C18 column was used.…”

Section: Discussionmentioning

confidence: 99%

“…1A). Additional HPLC methods have been used for uremic patients, but these methods have tedious and time-consuming procedures (12,13). …”

Section: Introductionmentioning

confidence: 99%

A modified HPLC method improves the simultaneous determination of plasma kynurenine and tryptophan concentrations in patients following maintenance hemodialysis

Xiao

Chen

Liang

et al. 2014

Experimental and Therapeutic Medicine

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show abstract

“…Concentrations of Trp, KYN and KYNA were measured as described previously, with some modifications –. In brief, we used a sensitive and accurate method that can simultaneously measure serum Trp, KYN and KYNA using HPLC with ultraviolet (UV) and fluorescence detection (FD).…”

Section: Methodsmentioning

confidence: 99%

Negative effects on newborn piglets caused by excess dietary tryptophan in the morning in sows

Bai

Peng

et al. 2019

J Sci Food Agric

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BACKGROUND This study investigated the effect of dynamic feeding models of dietary tryptophan on sows' performance during late pregnancy. RESULTS The average piglet birth weight and live farrowing rate from sows consuming a high–low tryptophan diet (0.39% Trp in the morning and 0.13% Trp in the afternoon) were decreased compared with those fed a 2×tryptophan diet (0.26% Trp in the morning and afternoon). Compared with the 2×tryptophan group, sow serum kynurenic acid and the newborn liver n‐6:n‐3 polyunsaturated fatty acid ratio were significantly higher, and sow serum taurine and newborn serum taurine, phosphoserine, cysteine and proline were lower in the high–low tryptophan diet group. Eighty‐eight genes were differentially expressed in newborn piglets' livers between the 2×tryptophan and high–low groups. Genes related to cytotoxic effector regulation (major histocompatibility complex class I proteins), NADH oxidation, reactive oxygen species (ROS) metabolism and tissue development were differentially expressed between these two groups. CONCLUSION Together, the results provide information on new biomarkers in serum or liver and provide novel insights into variations in the fetal liver during exogenous stimulus response and biological processes of ROS metabolism in fetuses during late pregnancy caused by a single excessive tryptophan ingestion daily in the morning. © 2018 Society of Chemical Industry

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“…Hence, it becomes extraordinarily important to develop rapid, available and practical methods for the chiral identification of Trp enantiomers. Miscellaneous detection techniques of Trp enantiomers have been reported, such as high performance liquid chromatography 7, fluorescence 8, quartz crystal microbalance 9, colorimetric analysis 10 and electrochemical detection 11. In comparison with other potential methods, electrochemical methods are increasingly becoming an important tool for researching chiral recognition because of its speed, simple operation, high sensitivity and low cost.…”

Section: Introductionmentioning

confidence: 99%

A Simple Non‐Gassing, Direct Current, Electro‐Osmotic Pump: Carbon Paper/Ceramic Frit/Carbon Paper

Nagarale

Heller

2014

ChemElectroChem

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We report the simplest of the small, non-gassing, low-voltage, direct-current, electro-osmotic pumps, providing enough flow for the delivery of drugs. It consists merely of a ceramic frit sandwiched between two plasma-treated carbon paper electrodes; the plasma treatment adds electro-oxidizable hydroquinone and electro-reducible quinone surface functions. Protons released upon the electro-oxidation of the hydroquinone surface functions move concertedly in the applied electric field at the ceramic membrane water interface and by dragging bulk water, causing water to flow at a rate of 10 mL cm À2 min À1 at an applied potential of 1 V across a 2 mm-thick membrane. Following plasma treatment of the carbon paper for 45 min, the flow persists for 18 h, in which 10 mL cm À2 of water is pumped.Most electro-osmotic pumps were built in the past with platinum-containing electrodes, and ceramic frit membranes were sandwiched between the electrodes. As platinum catalyzes the electro-reduction of water to H 2 and its electro-oxidation to O 2, the evolved gas bubbles adhering to the electrodes unpredictably affect the current flowing at a constant applied voltage, and the bubbles trapped in the ceramic frit randomly decrease the flow rate of water. Power expended on the electrolysis of water also decreases the pumping efficiency. Non-gassing, lowvoltage, electro-osmotic pumps have previously been built with poly(3,4-ethylenedioxythiophene), [1]

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Simultaneous determination of tryptophan, kynurenine and 5-hydroxytryptamine by HPLC: Application in uremic patients undergoing hemodialysis

Cited by 55 publications

References 31 publications

A modified HPLC method improves the simultaneous determination of plasma kynurenine and tryptophan concentrations in patients following maintenance hemodialysis

A modified HPLC method improves the simultaneous determination of plasma kynurenine and tryptophan concentrations in patients following maintenance hemodialysis

Negative effects on newborn piglets caused by excess dietary tryptophan in the morning in sows

A Simple Non‐Gassing, Direct Current, Electro‐Osmotic Pump: Carbon Paper/Ceramic Frit/Carbon Paper

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