Simultaneous determination of triprolidine and pseudoephedrine in human plasma by liquid chromatography–ion trap mass spectrometry

Shakya, Ashok K.; Arafat, Tawfiq; Abuawwad, A. N.; Melhim, Munther; Al-Ghani, Jafar; Yacoub, Mahmoud

doi:10.1016/j.jchromb.2009.10.021

Cited by 14 publications

(9 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The published chromatographic methods for the determination of antihistamines include mainly liquid–liquid extraction (LLE) and solid‐phase extraction (SPE) procedures or direct injection into the chromatographic system (Tables ). Protein precipitation of the samples with methanol (Ming et al , ), acetonitrile (Naidong et al , ; Gupta et al , ; Vlase et al , ), HClO 4 25% (Moncrieff, ) or trichloroacetic acid 5–6% (Lange et al , ; Shakya et al , ; Wichitnithad et al , ) have been used prior to LC analysis.…”

Section: Analytical Methods For the Determination Of Antihistamines Imentioning

confidence: 99%

“…The published chromatographic methods for the determination of antihistamines include mainly liquid-liquid extraction (LLE) and solid-phase extraction (SPE) procedures or direct injection into the chromatographic system (Tables 2-5). Protein precipitation of the samples with methanol (Ming et al, 2007), acetonitrile (Naidong et al, 2002;Gupta et al, 2005;Vlase et al, 2007), HClO 4 25% (Moncrieff, 1992) or trichloroacetic acid 5-6% (Lange et al, 1968;Shakya et al, 2009;Wichitnithad et al, 2015) have been used prior to LC analysis. Solans et al (2007) subjected plasma samples to enzymatic hydrolysis with β-glucuronidase/arylsulfatase from Helix pomatia in order to quantify free and glucuronoconjugated 3-hydroxydesloratadine, a common metabolite of desloratadine and rupatadine.…”

Section: Pretreatment Proceduresmentioning

confidence: 99%

See 1 more Smart Citation

Bioanalysis of antihistamines for clinical or forensic purposes

Katselou

Papoutsis

Νικολάου

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

Antihistamines are a class of drugs that inhibit the action of histamine and are used to alleviate symptoms associated with allergic reactions, but some of them can cause side effects, the most unpleasant and dangerous of which are the sedative effects that may hinder important psychological functions and impair skilled performance. These side effects could decrease safety in certain common and critical tasks, such as driving or operating machinery, leading to accidents. Antihistamines can also cause intoxications, sometimes lethal, especially when co-administered with alcohol or other sedative drugs. Thus, the development of analytical methods for their determination in biological fluids is considered to be useful for the investigation of clinical and forensic cases. These methodologies could also be used for pharmacokinetic studies. Several liquid and a few gas chromatographic methods have been developed for the determination of antihistamines in biological matrices after proper pretreatment procedures. This article reviews the published analytical methodologies that were gathered through the search in PubMed database and the recent developments on isolation or determination of antihistamines in biological materials. Current trends and future perspectives on bioanalysis of antihistamines are also discussed. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Section: Analytical Methods For the Determination Of Antihistamines Imentioning

confidence: 99%

Section: Pretreatment Proceduresmentioning

confidence: 99%

Bioanalysis of antihistamines for clinical or forensic purposes

Katselou

Papoutsis

Νικολάου

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Assessmentof the intra-and inter-dayv ariability of the assayw asconducted in differentlots of humanp lasma spiked withp seudoephedrine overt he calibration range of 10-500 ng/mL.The intra-and inter-dayprecision and accuracyw ered etermined byanalyzing plasma samplesatt he concentrationsof 10,20,100 and 500 ng/mL.The results of intra-and inter-dayprecision and accuraciesforpseudoephedrine in humanp lasma arepresented in Table 2.The accuracyof pseudoephedrine wasexpressed asapercentage of the measured concentration tothe theoreticalconcentration ranging from 97.6t o1 02.7%f orintra-dayand from 99.36 to 100.31% forinter-day,respectively.The precision (C.V.) of pseudoephedrine ranged from 3.99 to1 1.82%f orintra-dayand from 7.65to1 8.42%f orinter-day,respectively.Additionally,the stability of thismethod should havebeen tested in thiss tudy.However,from the inter-dayr esults of precision and accuracyfor5days itis expected thatt hisanalyticalm ethod canbep roperly and stablyapplied fort he determination of pseudoephedrine in humanplasma.Manyreports confirmthat the stability of pseudoephedrine ismaintained during analysis [14,15].Ino rdert oconfirmthe stability of pseudoephedrine in thismethod,afurthers tudyt oi nvestigatethe stability in various conditionsisneeded.…”

Section: Precision and Accuracymentioning

confidence: 68%

Reverse-phase liquid chromatography with electrospray ionization/mass spectrometry for the quantification of pseudoephedrine in human plasma and application to a bioequivalence study

Kim¹,

Jee²,

Park

et al. 2011

Arzneimittelforschung

View full text Add to dashboard Cite

A sensitive and selective reverse-phase liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) method was developed and validated to quantify pseudoephedrine (CAS 90-82-4) in human plasma. Phenacetin was used as the internal standard (I.S.). Sample preparation was performed with a deproteinization step using acetonitrile. Pseudoephedrine and I.S. were successfully separated using gradient elution with 0.5% trifluoroacetic acid (TFA) in water and 0.5% TFA in methanol at a flow-rate of 0.2 mL/min. Detection was performed on a single quadrupole mass spectrometer by a selected ion monitoring (SIM) mode via electrospray ionization (ESI) source. The ESI source was set at positive ionization mode. The ion signals of m/z 166.3 and 180.2 were measured for the protonated molecular ions of pseudoephedrine and I.S., respectively. The lower limit of quantification (LLOQ) of pseudoephedrine in human plasma was 10 ng/mL and good linearity was observed in the range of concentrations 10-500 ng/mL (R2 = 1). The intra-day accuracy of the drug containing plasma samples was more than 97.60% with a precision of 3.99-11.82%. The inter-day accuracy was 99.36% or more, with a precision of 7.65-18.42%. By using this analytical method, the bioequivalence study of the pseudoephedrine preparation was performed and evaluated by statistical analysis of the log transformed mean ratios of pharmacokinetic parameters. All the results fulfilled the standard criteria of bioequivalence, being within the 80-125% range which is required by the Korea FDA, US FDA, and EMEA to conclude bioequivalence. Consequently, the developed reverse-phase LC-ESI-MS method was successfully applied to bioequivalence studies of pseudoephedrine in healthy male volunteers.

show abstract

“…Combined dosage forms of Triprolidine and Pseudoephedrine Hydrochloride are official in United State Pharmacopoeia 23 and HPLC method is given for their simultaneous estimation. Literature survey reveals that HPLC methods [24][25][26][27] and UV-spectrophotometric method [28][29] has been reported for simultaneous estimation of Triprolidine and Pseudoephedrine Hydrochloride.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric and High Performance Liquid Chromatographic Determination (HPLC) of Triprolidine and Pseudoephedrine Hydrochloride in Tablet Dosage Form

Hinge¹,

Patel²,

Mahida³

2015

PHARM METH

View full text Add to dashboard Cite

First order derivative spectrophotometric method and high performance liquid chromatographic method were developed for the determination of Triprolidine and Pseudoephedrine Hydrochloride in tablet dosage form. In UV-Spectrophotometric method, estimation of Triprolidine and Pseudoephedrine Hydrochloride was carried out at the wavelength selected 246.20 nm and 263.50 nm for First order Derivative method. Calibration curves were linear in the range of 2-10 μg ml-1 for Triprolidine and 48-240 μg ml-1 for Pseudoephedrine Hydrochloride in derivative method. Correlation coefficient found to be close to 0.9950 for both the drugs. Accuracy for both the drugs was in the range of 99-101.5%. A simple liquid chromatographic assay has been developed for the determination of Triprolidine and Pseudoephedrine Hydrochloride. A C 18 (250×4.6 mm, 5 μm) column was used with a mobile phase consisting of Methanol: Water (80: 20 v/v) (pH adjusted to 3.0 with ortho phosphoric acid) at a flow rate of 1.0 ml min-1. Quantitation was achieved with UV detection at 246.20 nm based on the peak height ratios. Beer's law was obeyed in a concentration range of 5-25 µg ml-1 for Triprolidine and 120-600 µg ml-1 for Pseudoephedrine Hydrochloride and the regression line equation was derived with a correlation coefficient of 0.9999 and 0.9998 for Triprolidine and Pseudoephedrine Hydrochloride. The proposed procedures were successfully applied to the determination of Triprolidine and Pseudoephedrine Hydrochloride in bulk and tablet form, with high percentage of recovery, good accuracy and precision.

show abstract

Simultaneous determination of triprolidine and pseudoephedrine in human plasma by liquid chromatography–ion trap mass spectrometry

Cited by 14 publications

References 22 publications

Bioanalysis of antihistamines for clinical or forensic purposes

Bioanalysis of antihistamines for clinical or forensic purposes

Reverse-phase liquid chromatography with electrospray ionization/mass spectrometry for the quantification of pseudoephedrine in human plasma and application to a bioequivalence study

Spectrophotometric and High Performance Liquid Chromatographic Determination (HPLC) of Triprolidine and Pseudoephedrine Hydrochloride in Tablet Dosage Form

Contact Info

Product

Resources

About