Simultaneous determination of triazolam and its metabolites in human plasma by liquid chromatography–tandem mass spectrometry

Pan, Ryh‐Nan; Lin, Chang‐Ching; Huang, Pei-Wei; Hsiong, Cheng-Huei; Pao, Li-Heng

doi:10.1016/j.jchromb.2008.07.014

Cited by 13 publications

(6 citation statements)

References 23 publications

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“…Urine samples were prepared by solid-phase extraction (SPE). The SPE protocol from our previous study was followed with some modification (12). The samples were stored as 1 mL aliquots at -80°C; the aliquots were thawed at room temperature before analysis.…”

Section: Sample Preparationmentioning

confidence: 99%

“…In order to improve the detection ability of abused drugs like triazolam and study its pharmacokinetics, a selective and sensitive analytical method is essential. Several methods have been reported for the analysis of triazolam in biological fluids, including gas chromatography (GC) (7), GC-mass spectrometry (MS) (8), high-performance liquid chromatography (HPLC) (9,10), LC-MS (4,11), and an LC-MS-MS method that was developed in our laboratory (12).…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Quantification of Triazolam and its Metabolites in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry

Pan

Hsiong

Huang

et al. 2011

Journal of Analytical Toxicology

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A simple, simultaneous, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the determination of triazolam and its metabolites, α-hydroxytriazolam (α-OHTRZ) and 4-hydroxytriazolam (4-OHTRZ), in human urine was developed and validated. Triazolam-d4 was used as the internal standard (IS). This analysis was carried out on a Thermo(®) C(18) column, and the mobile phase was composed of acetonitrile/H(2)O/formic acid (35:65:0.2, v/v/v). Detection was performed on a triple-quadrupole tandem MS using positive ion mode electrospray ionization, and quantification was performed by multiple reaction monitoring mode. The MS-MS ion transitions monitored were m/z 343.1 → 308.3, 359.0 → 331.0, 359.0 → 111.2, and 347.0 → 312.0 for triazolam, α-OHTRZ, 4-OHTRZ, and triazolam-d(4), respectively. The lower limits of quantification of the analytical method were 0.5 ng/mL for triazolam, 5 ng/mL for α-OHTRZ, and 0.5 ng/mL for 4-OHTRZ. The within- and between-run precisions were less than 15%, and accuracy was -12.33% to 9.76%. The method was proved to be accurate and specific, and it was applied to a urinary excretion study of triazolam in healthy Chinese volunteers.

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Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Triazolam and its Metabolites in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry

Pan

Hsiong

Huang

et al. 2011

Journal of Analytical Toxicology

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“…Additionally, they have both hydrophilic and lipophilic properties and are capable of capturing polar analytes. This advantage for metabolites, which are usually more polar, has been shown by several research groups (Allqvist et al , ; Quintela et al , ; Feng et al , ; Fuh et al , ; Pan et al , ; Nakamura et al , ). Other groups have used a mixed‐mode polymer with a cation‐exchange function (Oasis MCX or Isolute HCX) for sample preparation (Kollroser and Schober, ; Jourdil et al , ; Chin et al , ; Harris et al , ; Hegstad et al , ; Miyaguchi et al , ; Lennestal et al , ).…”

Section: Sample Preparationmentioning

confidence: 81%

Analyses of benzodiazepines and their metabolites in various biological matrices by LC-MS(/MS)

Nakamura

2011

Biomed. Chromatogr.

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Benzodiazepines are among the most frequently prescribed drugs due to their sedative, hypnotic, anxiolytic, muscle relaxant and antiepileptic properties. Because of the high consumption of benzodiazepines worldwide, this class of drugs and their metabolites are frequently present in both clinical and forensic cases. For these reasons, the analysis of benzodiazepines and their metabolites in biological fluids is of great interest to clinicians and forensic toxicologists. This paper reviews procedures for multi-analyte single-stage (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) using different mass analyzers for the screening, identification and/or quantification of drugs, poisons and/or their metabolites in blood, plasma, serum or urine published since 2001. Basic information about the biosamples assayed, work-up, LC column, mobile phase, ionization type, mass spectral detection mode, matrix effects and validation data for each procedure is summarized. The feasibility of using LC-MS(/MS) techniques to identify and quantify benzodiazepines and their metabolites is also discussed.

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“…Recent examples of the use of MRM for analysing endogenous compounds in humans include the measurement of vitamins [52], steroids [53][54][55] and neurotransmitters [56,57]. MRM analyses of drugs and their metabolites are too numerous to list comprehensively, but include the measurement of therapeutic agents, such as warfarin [58], triazolam [59], nevirapine [60] and antibiotics [61], the measurement of drugs of The targeted parent ion is selected in the first quadrupole (Q1) and enters the second quadrupole (Q2) where it undergoes collision-induced dissociation. One or more fragment ions are then selected according to the predefined transitions and the ensuing signal provides the spectral counts for quantification.…”

Section: Use Of Multiple Reaction Monitoring For the Analysis Of Smalmentioning

confidence: 99%

Multiple reaction monitoring for quantitative biomarker analysis in proteomics and metabolomics☆

Kitteringham

Jenkins

Lane

et al. 2009

Journal of Chromatography B

308

246

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Simultaneous determination of triazolam and its metabolites in human plasma by liquid chromatography–tandem mass spectrometry

Cited by 13 publications

References 23 publications

Simultaneous Quantification of Triazolam and its Metabolites in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Quantification of Triazolam and its Metabolites in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry

Analyses of benzodiazepines and their metabolites in various biological matrices by LC-MS(/MS)

Multiple reaction monitoring for quantitative biomarker analysis in proteomics and metabolomics☆

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