Simultaneous Determination of Tizanidine, Nimesulide, Aceclofenac and Paracetamol in Tablets and Biological Fluids Using Micellar Liquid Chromatography

Abstract

A simple, sensitive and rapid micellar liquid chromatographic method was developed and validated for simultaneous determination of four drugs, namely, paracetamol (PAR), tizanidine (TZD), aceclofenac (ACF) and nimesulide (NMD). Good chromatographic separation was achieved using Cyano column and micellar mobile phase consisting of 120 mM sodium dodecyl sulfate, 25 mM phosphate buffer and 10% (V/V) butanol. The pH was adjusted to three using phosphoric acid. The total retention time was below 10 min. The analysis was performed at a flow rate of 1 mL/min and a column temperature of 40°C with direct UV detection at 230 nm. Diclofenac sodium was used as the internal standard. The proposed method was validated according to the ICH guidelines and was successfully applied to the analysis of these drugs in their tablet dosage forms with high accuracy. Limits of detection were found to be 0.03, 0.07, 0.033 and 0.11 μg/mL for PAR, ACF, TZD and NMD, respectively. The high sensitivity of developed method permitted its application to the in-vitro determination of the cited drugs in spiked human plasma and urine samples, and the obtained results were satisfactory. However, PAR could not be determined in spiked human urine because its peak overlapped with that of the urine peak.

Earth-friendly micellar UPLC technique for determination of four hypoglycemic drugs in different pharmaceutical dosage forms and spiked human plasma

“…A Straightforward and well-established branch of high-performance liquid chromatography is micellar liquid chromatography (MLC). MLC has been used frequently to identify various substances in pharmaceutical medications [ 35 , 36 ], biological fluids [ 37 , 38 ], and food products [ 39 ].…”

Using Carbon Paste Electrode Modified with Graphene and Nanodiamond for the Determination of Nimesulide in Biologic and Environmental Samples

“…Considering its widespread use and the risks it poses to humans and the environment, it is essentially important to develop highly efficient analytical techniques capable of determining the presence of NIM in biological and environmental samples. Previous studies reported in the literature have employed a variety of techniques for the analytical determination of NIM in different matrices; some of the techniques employed include chromatography [10][11], spectrometry [12][13], and electroanalysis [14][15][16].…”

On-off Fluorescent Switching of Excitation-independent Near-ultraviolet Emission Carbon Nanobelts for Ultrasensitive Detection Nimesulide in Pharmaceutical Tablet

“…5 However, the uncontrolled and improper use of Nim in real tablets might cause great harm to human health, such as gastrointestinal problems, liver and kidney damage, and so on. [6][7][8] Therefore, it is of decisive significance to detect Nim levels in real tablets for the sake of health. So far, a plethora of methods, including electrochemical methods, 3,9 highperformance liquid chromatography-mass spectrum, 10 micellar liquid chromatography, 7 spectrophotometric determination 11 and fluorescence techniques, 5,12 have been used to monitor Nim.…”

“…[6][7][8] Therefore, it is of decisive significance to detect Nim levels in real tablets for the sake of health. So far, a plethora of methods, including electrochemical methods, 3,9 highperformance liquid chromatography-mass spectrum, 10 micellar liquid chromatography, 7 spectrophotometric determination 11 and fluorescence techniques, 5,12 have been used to monitor Nim. Among the above analysis methods, the luminescence sensing platform has aroused great interest due to its fast detection, simplicity, high sensitivity and selectivity; what is more, they do not involve any complicated pretreatment process and do not destroy biological samples.…”