Simultaneous determination of timolol maleate, rosuvastatin calcium and diclofenac sodium in pharmaceuticals and physiological fluids using HPLC-UV

Nasir, Fazli; Iqbal, Zafar; Khan, Abad; Ahmad, Lateef; Shah, Yatrik M.; Khan, Aman Nawaz; Khan, Jamil; Khan, Shahroz

doi:10.1016/j.jchromb.2011.09.021

Cited by 56 publications

(32 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, using ion paring LLE, extraction efficacy of rosuvastatin in ethyl acetate was improved from around 10% to more than 50%. A somewhat unusual LLE method for determination of timolol maleate, rosuvastatin and diclofenac in human plasma and aqueous humor from the bovine eyes was proposed [81]. The mobile phase, consisted of acetonitrile and 0.2% triethylamine, was used as extraction solvent.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Their typical plasma concentrations are in ng/mL levels. However, several sensitive LC/DAD methods for determination of pravastatin [31], atorvastatin [71], lovastatin [73], rosuvastatin [81], and atorvastatin with rosuvastatin [90] have been developed with limit of quantification (LOQ) in range of 1 -10 ng/mL. Less sensitive LC/DAD method for quantification of lovastatin in human plasma was developed [91].…”

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

“…Therefore, these methods could be useful in daily routine sample handling, when many samples from patients taking different drugs together with HMG-CoA reductase inhibitors are analyzed in clinical laboratories. Recently, several chromatographic methods have been developed for the quantification of statin drugs in combination with other drugs, most of them are commonly used in treatment of cardiovascular disease: atenolol, spironolactone, glibenclamide [61], metoprolol succinate [72], valsartan and chlorthalidone [76,87], timolol maleate, diclofenac sodium [81], fenofibric acid [85], ezetimibe [91], ceftriaxone [92], acetylsalicylic acid [94], amlodipine [98] and losartan, atenolol, acetylsalicylic acid [99].…”

Section: Pharmacokinetic Studiesmentioning

confidence: 99%

See 2 more Smart Citations

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

Nigović¹,

Mornar²,

Sertić³

2012

Chromatography - The Most Versatile Method of Chemical Analysis

View full text Add to dashboard Cite

Section: Sample Preparationmentioning

confidence: 99%

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Section: Pharmacokinetic Studiesmentioning

confidence: 99%

See 1 more Smart Citation

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

Nigović¹,

Mornar²,

Sertić³

2012

Chromatography - The Most Versatile Method of Chemical Analysis

View full text Add to dashboard Cite

“…11,12,26,27 The A solvent was acetonitrile and B solvent was 50 mM acetate buffer (1/1), (v/v) pH 3.1. The column temperature was stable at 25°C.…”

Section: Ultra Performance Liquid Chromatography Assay Methods and Valmentioning

confidence: 99%

Development and Validation of an Ultra Performance Liquid Chromatography Method for the Determination of Dexketoprofen Trometamol, Salicylic Acid and Diclofenac Sodium

Ilbasmiş-Tamer

2017

tjps

View full text Add to dashboard Cite

ÖZAmaç: Deksketoprofen trometamol (DKP), salisilik asit (SA) ve diklofenak sodyumun (DIC) ilaç çözeltisindeki analizi için ultra yüksek basınçlı sıvı kromatografisi (UPLC) kullanılarak basit, hızlı, doğru ve kesin bir yöntem geliştirilmiştir. Gereç ve Yöntemler: UPLC yöntemi aktif bileşikleri analiz etmek için oldukça hassas bir yöntemdir ve yöntem ICH kurallarına göre valide edilmiştir. Bulgular: Geliştirilen yöntem, kesin, doğru, spesifik ve seçici bulunmuştur. Yöntem, doğrusal ve tekrarlanabilir bulunmuştur. DKP maddesi için teşhis sınırı-duyarlılık sınırı (LOD) 0.00325 µg/mL ve tayin alt sınırı-saptama sınırı (LOQ) 0.00985 µg/mL olarak bulunmuştur. SA için LOD 0.0027 µg/mL ve LOQ 0.0081 µg/mL olarak bulunmuştur. DIC için LOD 0.0304 µg/mL ve LOQ 0.0920 µg/mL olarak bulunmuştur. Sonuç: Böylece önerilen yöntemler yukarıda bahsedilen ilaçları içeren (deksketoprofen trometamol, salisilik asit ve diklofenak sodyum) farmasötik preparatlarda başarılı bir şekilde uygulanabilecektir. Aktif bileşikler çok küçük miktarlarda analiz edilebilecek ve kolaylıkla valide edilebilecektir. Anahtar kelimeler: Deksketoprofen trometamol, salisilik asit, diklofenak sodyum, UPLC, validasyon Objectives: A simple, fast, accurate and precise method has been developed for the determination of dexketoprofen trometamol (DKP), salicylic acid (SA) and diclofenac sodium (DIC) in the drug solutions using ultra high performance liquid chromatography (UPLC). Materials and Methods: UPLC method is highly reliable and sensitive method to quantify the amount of the active ingredient and the method is validated according to ICH guidelines. Results: The developed method is found to be precise, accurate, specific and selective. The method was also found to be linear and reproducible. The value of limit of dedection (LOD) of DKP, SA, DIC were found 0.00325 µg/mL, 0.0027 µg/mL and 0.0304 µg/mL, respectively. The limit of quantitation (LOQ) of DKP, SA and DIC were found 0.00985 µg/mL, 0.0081 µg/mL and 0.0920 µg/mL, respectively. Conclusion: Proposed methods can be successfully applicable to the pharmaceutical preparation containing the above mentioned drugs (dexketoprofen trometamol, salicylic acid and diclofenac sodium). Even very small amounts of active substance can be analyzed and validations can be performed easily.

show abstract

“…In this work, the HPLC analysis was performed in isocratic mode on a reverse phase Hypersil BDS C 18 column (5 μm, 250 mm × 4.6 mm i.d., Thermo Scientific, IL, USA). The flow rate and column temperature were set at 0.8 ml/min and 20°C, respectively, and the peaks were detected at a wavelength of 275 nm [17].…”

Section: In Vivo Pharmacokinetic Studymentioning

confidence: 99%

Implantable micro-chip for controlled delivery of diclofenac sodium

Lee

Park

et al. 2014

Journal of Controlled Release

View full text Add to dashboard Cite

Simultaneous determination of timolol maleate, rosuvastatin calcium and diclofenac sodium in pharmaceuticals and physiological fluids using HPLC-UV

Cited by 56 publications

References 27 publications

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

Development and Validation of an Ultra Performance Liquid Chromatography Method for the Determination of Dexketoprofen Trometamol, Salicylic Acid and Diclofenac Sodium

Implantable micro-chip for controlled delivery of diclofenac sodium

Contact Info

Product

Resources

About