Simultaneous Determination of Three Coumarins in Angelica dahurica by ¹H-qNMR Method: A Fast and Validated Method for Crude Drug Quality Control

Abstract

In this study, a quantitative 1 H NMR method ( 1 H-qNMR) for determining the contents of imperatorin, byakangelicin, and oxypeucedanin in A. dahurica in traditional Chinese medicine (TCM) has been established. Dried plant material was extracted exhaustively with methanol by an ultrasonication-assisted extraction method. e 1 H-qNMR measurements were performed on a 600 -MHz spectrometer with hydroquinone as the internal standard reference in deuterated dimethyl sulfoxide (DMSO-d 6 ) solvent. Quantification was carried out using the 1 H resonance signals at 6.55 ppm for hydroquinone and 7.68, 7.38-7.39, and 6.38-6.39 ppm for imperatorin, byakangelicin, and oxypeucedanin, respectively. e linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, reproducibility, stability, and recovery of the methodology were evaluated, and results were good. e newly developed method has been applied to determine the three coumarins in A. dahurica.

Quantitative Analysis of Flavonoids in Glycyrrhiza uralensis Fisch by 1H-qNMR

“…Nuclear magnetic resonance (NMR) spectroscopy is commonly used for structure elucidation, but the potential for its quantitative ability is increasing nowadays. The 1 H-qNMR method could simultaneously detect multiple components in very short time (1–5 mins) using a very cheap internal standard reference, which has found applications in quantitative analysis of natural products and other areas [ 12 , 17 – 34 ]. In our previous study, we have developed a quantitative NMR (qNMR) method for Angelica dahurica and Angelicae Pubescentis Radix analysis [ 35 , 36 ].…”

Oxypeucedanin: Chemotaxonomy, Isolation, and Bioactivities

“…Supercritical fluid chromatography (SFC) has been utilized to analyze the methanolic partitions of the five root samples of A. dahurica , and OP was subsequently quantified at 1.54–2.93 g/100 g [ 77 ]. In a recent study performed by Yang et al [ 78 ], quantitative 1 H-NMR was exploited to examine 11 Chinese plant samples, and the OP amount ranged from 0.17 to 0.35%.…”

Rapid Quantification and Validation of Biomarker Scopoletin in Paederia foetida by qNMR and UV–Vis for Herbal Preparation

“…Relaxation delays of 2, 5, 20, 40, 60, and 120 s were conducted on the integral area. The integral area showed almost no difference and no influence on the quantitative data [13]. Therefore, 60 s was chosen as the relaxation delay.…”