Simultaneous Determination of Ten Active Components in 12 Chinese <i>Piper</i> Species by HPLC

Cai, Cheng-Cheng; Yan, Macheng; Xie, Hui; Pan, Shengli

doi:10.1142/s0192415x11009391

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…The dried stems of P. laetispicum (20 kg) were powdered and percolated with 95 % EtOH (Ethyl alcohol), and the solution was concentrated under reduced pressure to give crude extract (1510 g). The ethyl acetate extract (EAE-P) was obtained following the methods published by our group [ 9 ] [ 15 ]. The HPLC profile was the same with Xie et al [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

The effect of Piper laetispicum extract (EAE-P) during chronic unpredictable mild stress based on interrelationship of inflammatory cytokines, apoptosis cytokines and neurotrophin in the hippocampus

Xie

Jin

Kang

et al. 2015

BMC Complement Altern Med

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BackgroudThe Piper laetispicum C.DC. (Piperaceae) is a traditionally used herb in China for invigorating circulation and reducing stasis, detumescence and analgesia, which is distributed in the southern part of China and the southeastern part of Asia. Previous studies demonstrated that the ethyl acetate extract (EAE-P) of P. laetispicum possesses a significant antidepressant-like effect at doses higher than 60 mg/kg in Kunming (KM) mice, and this effect was not due to an increase in locomotive activity.MethodsTo research this mechanism, in the present study, the chronic unpredictable mild stress (CUMS) model in Sprague–Dawley rats was used to further elucidate behavioral changes and corresponding changes in inflammatory cytokines (TNF-α, IL-6, IL-10), apoptosis cytokines (P53, Bax, Bcl2, caspase-3) and neurotrophin (BDNF) in the hippocampus of EAE-P treatment animals.Results and conclusionsThe results suggest that EAE-P is beneficial to the behavioral outcome of the CUMS model animals, and decreased amounts of inflammatory cytokine IL-6 contributed to the antidepressant-like activation of EAE-P in every dosage group (15, 30, 60 mg/kg). In the low dosage group, down-regulated apoptosis cytokine p53 is associated with EAE-P effect, but it is inflammatory cytokine TNF-α that is related to the effect of EAE-P in the high dosage group. Meanwhile, the P53-dependent antiapoptotic effect of EAE-P may not be through Bcl-2 and Bax modulation. Furthermore, EAE-P showed up-regulated expression of brain-derived neurotrophic factor (BDNF) mRNA and down-regulated apoptosis cytokine caspase-3 mRNA, which was the same change tendency as with Fluoxetine.

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Section: Methodsmentioning

confidence: 99%

The effect of Piper laetispicum extract (EAE-P) during chronic unpredictable mild stress based on interrelationship of inflammatory cytokines, apoptosis cytokines and neurotrophin in the hippocampus

Xie

Jin

Kang

et al. 2015

BMC Complement Altern Med

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“…Its effectiveness has been recognized by the Chinese people (Wang et al, 2011b;Xutian et al, 2012). The highquality of Chinese herbal medicine is important to ensure the efficacy of TCM (Cai et al, 2011;Chen et al, 2011). In recent years, the dramatic increase in the production and decline in quality of Chinese herbal medicines resulted in a crisis in the development of TCM.…”

Section: Introductionmentioning

confidence: 99%

Arbuscular Mycorrhizal Symbiosis for Sustainable Cultivation of Chinese Medicinal Plants: A Promising Research Direction

et al. 2013

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Arbuscular mycorrhizal (AM) are symbiotic systems in nature and have great significance in promoting the growth and stress resistance of medicinal plants. During our literature searchfrom the Chinese Scientific Information Database (Chinese National Knowledge Infrastructure, CNKI) we obtained 65 articles with "AM fungi" and "medicinal plant" as the key words, which indicates that in China, research efforts on these topics have been increasing. The main purposes of this review are to discuss the effects of mycorrhiza on the active ingredients of Chinese medicinal plants in comparison with results obtained in other plants in studies conducted by the international research community, and to introduce works published in Chinese journals to international colleagues.

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“…Furthermore, this pretreatment outcome is more complicated by using TCM herbal formulations, because a formulation often consists of several different medicinal herbs. Herbal quality control consists of several aspects (Cai et al, 2011; Wang et al, 2011), and extraction pretreatment further complicated the quality control of TCM therapies.…”

Section: Discussionmentioning

confidence: 99%

Different extraction pretreatments significantly change the flavonoid contents ofScutellaria baicalensis

Mao

et al. 2013

Pharmaceutical Biology

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Context Scutellaria baicalensis is one of the most commonly used medicinal herbs, especially in traditional Chinese medicine. However, compared to many pharmacological studies of this botanical, much less attention has been paid to the quality control of the herb’s pretreatment prior to extract preparation, an issue that may affect therapeutic outcomes. Objective The current study was designed to evaluate whether different pretreatment conditions change the contents of its four major flavonoids in the herb, i.e., two glycosides (baicalin and wogonoside) and two aglycons (baicalein and wogonin). Materials and methods An HPLC assay was used to quantify the contents of these four flavonoids. The composition changes of four flavonoids by different pretreatment conditions including solvent, treatment time, temperature, pH value, and herb/solvent ratio were evaluated. Results After selection of the first order time-curve kinetics, our data showed that at 50°C, 1:5 herb/water (in w/v) ratio and pH 6.67 yielded an optimal conversion rate from flavonoid glycosides to their aglycons. In this optimized condition, the contents of baicalin and wogonoside were decreased to 1/70 and 1/13, while baicalein and wogonin were increased 3.5 and 3.1 folds, respectively, compared to untreated herb. Discussion and conclusion The markedly variable conversion rates by different pretreatment conditions complicated the quality control of this herb, mainly due to the high amount of endogenous enzymes of S. baicalensis. Optimal pretreatment conditions obtained from this study could be used obtain the highest level of desired constituents to achieve better pharmacological effects.

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Simultaneous Determination of Ten Active Components in 12 Chinese Piper Species by HPLC

Cited by 6 publications

References 22 publications

The effect of Piper laetispicum extract (EAE-P) during chronic unpredictable mild stress based on interrelationship of inflammatory cytokines, apoptosis cytokines and neurotrophin in the hippocampus

The effect of Piper laetispicum extract (EAE-P) during chronic unpredictable mild stress based on interrelationship of inflammatory cytokines, apoptosis cytokines and neurotrophin in the hippocampus

Arbuscular Mycorrhizal Symbiosis for Sustainable Cultivation of Chinese Medicinal Plants: A Promising Research Direction

Different extraction pretreatments significantly change the flavonoid contents ofScutellaria baicalensis

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