Simultaneous determination of sweeteners in beverages by LC-MS/MS

Sakai, Hiroaki; Yamashita, Azusa; Tamura, Mieko; Uyama, Atsuo; Mochizuki, Naoki

doi:10.1080/19440049.2015.1018341

Cited by 18 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, none of the earlier studies measured as many soft drinks as our study did, nor similar soft drinks of the same company from batches obtained from different countries. Sakai et al also measured DKP in three soft drinks, ranging from 1.6 to 2.9 mg/L, which is comparable to concentrations found in our study [9]. The comparability of our results with those of other studies confirms the validity of our data.…”

Section: Discussionsupporting

confidence: 92%

“…The APM and total Phe concentrations in our 95 APM-containing drinks ranged from 103 to 1790 µ mol/L (30-527 mg/L) and from 119 to 2013 µ mol/L (20-332 mg/L), respectively. APM concentrations were to some extent comparable to concentrations reported earlier [9][10][11][12][13][14][15][16], except those reported by Zhu et al, who found significantly higher APM concentrations of 2826 and 7235 mg/L in two soft drinks [10]. However, none of the earlier studies measured as many soft drinks as our study did, nor similar soft drinks of the same company from batches obtained from different countries.…”

Section: Discussionsupporting

confidence: 88%

“…There are some publications on APM concentrations in a small number of soft drinks [9][10][11][12][13][14][15][16]. These studies analyzed APM mostly in addition to other sweeteners to investigate consumption or to study the instability of APM.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aspartame and Phe-Containing Degradation Products in Soft Drinks across Europe

et al. 2020

View full text Add to dashboard Cite

Phenylketonuria and tyrosinemia type 1 are treated with dietary phenylalanine (Phe) restriction. Aspartame is a Phe-containing synthetic sweetener used in many products, including many ‘regular’ soft drinks. Its amount is (often) not declared; therefore, patients are advised not to consume aspartame-containing foods. This study aimed to determine the variation in aspartame concentrations and its Phe-containing degradation products in aspartame-containing soft drinks. For this, an LC–MS/MS method was developed for the analysis of aspartame, Phe, aspartylphenylalanine, and diketopiperazine in soft drinks. In total, 111 regularly used soft drinks from 10 European countries were analyzed. The method proved linear and had an inter-assay precision (CV%) below 5% for aspartame and higher CVs% of 4.4–49.6% for the degradation products, as many concentrations were at the limit of quantification. Aspartame and total Phe concentrations in the aspartame-containing soft drinks varied from 103 to 1790 µmol/L (30–527 mg/L) and from 119 to 2013 µmol/L (20–332 mg/L), respectively, and were highly variable among similar soft drinks bought in different countries. Since Phe concentrations between drinks and countries highly vary, we strongly advocate the declaration of the amount of aspartame on soft drink labels, as some drinks may be suitable for consumption by patients with Phe-restricted diets.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

Aspartame and Phe-Containing Degradation Products in Soft Drinks across Europe

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Analysis and monitoring the content of sweeteners in food and beverage products using various methods (chromatographic, electrophoretic, and mass spectrometric methods, etc. ) are also the subject of many studies in the literature (Dacome et al 2005;Pane et al 2015;Sakai et al 2015). However, these current methods require the usage of expensive equipment, toxic chemicals and also long preparation steps.…”

Section: Introductionmentioning

confidence: 99%

Rapid Identification of Some Sweeteners and Sugars by Attenuated Total Reflectance-Fourier Transform Infrared (Atr-Ftir), Near-Infrared (Nir) and Raman Spectroscopy

Güven

Velioglu

Boyaci

2019

Gıda

View full text Add to dashboard Cite

The aim of the present study is to compare the vibrational characteristics of various sweeteners with different sugars by using attenuated total reflectance-Fourier transform infrared (ATR-FTIR), near-infrared (NIR) and Raman spectroscopy. For this purpose, ATR-FTIR, NIR and Raman spectra of several sugars and sweeteners were recorded. The spectra of all samples were examined in detail for each technique. Then certain values of sugars (sucrose, galactose, fructose, glucose) and sweeteners (saccharin, sucralose, neotame, acesulfame K and rebaudioside A) were compared. Also, the spectra of binary, triple and quaternary mixtures of some sweeteners and sugars were analyzed. The specific bands of each of the sugars and sweeteners were identified. The obtained spectral information can be used as a reference to develop useful spectral methods for routine controls of sweeteneers and sugars. Moreover, the methods will be helpful for the rapid identification of sweeteners and sugars.

show abstract

“…Several analytical methods based on HPLC with different detectors and pretreatment of samples have been developed for the determination of cyclamate, either individually or together with other sweeteners (Shah et al 2014(Shah et al , 2015Sakai et al 2015). Detection by indirect spectrophotometry (Herrmann et al 1983), absorption in the visible range with the addition of dyes (Choi et al 2000), absorption in the ultraviolet range (UV) and fluorescence prior to sample derivatisation (Hauck & Köbler 1990;Choi et al 2000), MS (Zygler et al 2011), a light-scattering evaporative detector (Wasik et al 2007), and capillary zone electrophoresis (Vistuba et al 2015) have been used.…”

Section: Introductionmentioning

confidence: 99%

Validation of an HPLC-DAD/UV method for the quantification of cyclamate in tabletop sweeteners: risk of exceeding the acceptable daily intake

Sargaço

Serra

Vasco

2017

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

Cyclamate is widely used as intense sweetener in the European Union. The absence of a maximum limit for the use of cyclamate in tabletop sweeteners and the growing demand for this type of product highlights the importance of developing robust analytical methods for the determination of its content to understand if the consumption of tabletop sweeteners can have a negative impact on human health. The present work aimed at the optimisation and validation of an high-performance liquid chromatography (HPLC) analytical method for cyclamate determination in tabletop sweeteners based on the procedure of European Standard EN 12857. The validated method was then applied to the determination of this sweetener in different types of tabletop sweeteners (liquid, powder and tablets). Both standards and samples solutions were submitted to a derivatisation procedure which converted cyclamate to N,N-dichlorocyclohexylamine. The derivatised product was separated and quantified using a reversed-phase column, a mobile phase composed of water (20%) and methanol (80%), isocratic flow of 1 ml min -1 , and detection by ultraviolet spectrophotometry at a wavelength of 314 nm. The analytical method was internally validated according to the following validation parameters: working range, linearity, limits of detection and quantification, sensitivity, precision (repeatability and intermediate precision), and uncertainty. This method proved to be specific and selective for the determination of this sweetener, showing repeatability, RSD r ≤ 3%, intermediate precision, RSD R ≤ 3.3%, and recovery rates from 92% to 108% for the different tabletop sweeteners. The method uncertainty was 9.4%. The concentration of cyclamate in the samples varied significantly, from 2.9% to 73.9%, which demonstrated that a possible excessive consumption of one of the analysed sweeteners can lead to exceeding the acceptable daily intake for cyclamate. ARTICLE HISTORY

show abstract

Simultaneous determination of sweeteners in beverages by LC-MS/MS

Cited by 18 publications

References 5 publications

Aspartame and Phe-Containing Degradation Products in Soft Drinks across Europe

Aspartame and Phe-Containing Degradation Products in Soft Drinks across Europe

Rapid Identification of Some Sweeteners and Sugars by Attenuated Total Reflectance-Fourier Transform Infrared (Atr-Ftir), Near-Infrared (Nir) and Raman Spectroscopy

Validation of an HPLC-DAD/UV method for the quantification of cyclamate in tabletop sweeteners: risk of exceeding the acceptable daily intake

Contact Info

Product

Resources

About