Simultaneous determination of six plant growth regulators in fruits using high performance liquid chromatography based on solid-phase extraction and cleanup with a novel mixed-mode functionalized calixarene sorbent

Abstract

Scheme of a SPE process.

Simultaneous determination of 10 plant growth promoters in fruits and vegetables with a modified QuEChERS based liquid chromatography tandem mass spectrometry method

“…Zhang et al 37 analyzed four plant growth regulators in soybean sprouts and mung bean sprouts by the QuEChERS-LC-MS/MS method, and the LODs of 2,4-D, GA3 and 6-BA were in the range of 0.27-9.3 mg kg À1 . Wang et al 38 developed a simultaneous determination method of six regulators, using a mixed-mode functionalized calixarene as a solid-phase extraction sorbent, and the LODs of 2,4-D, 1-naphthylacetic acid and 6-BA were 20.4, 4.3 and 10.6 mg kg À1 , respectively. Yan et al prepared a new molecularly imprinted polymer for extracting four plant hormones followed by HPLC detection, and the LODs of indole-3-butyric acid and 1-naphthylacetic acid were 3.8 and 3.0 mg kg À1 , respectively.…”

“…Furthermore, it has been successfully applied to the determination of multi-PGPs in real samples. 2,36,37 To improve the recoveries of target compounds, various modied versions of the QuEChERS method have been developed by modifying sorbents, such as primary secondary amines (PSAs), C 18 , calixarenes, 38 graphitized carbon black or Florisil, 39,40 etc. or using assisted extraction techniques, e.g.…”

Development and validation of LC-MS/MS method for simultaneous determination of 6-benzylaminopurine and forchlorfenuron in bean sprout

“…Nhiều phương pháp đã được xây dựng để xác định tồn dư các chất 6-BA và CPPU, trong đó phương pháp ELISA [4], LC-MS/MS kết hợp cùng quy trình xử lý mẫu bằng kỹ thuật SPE [5], QuEChERS [6][7]…”

ZIF‐8@SiO2 core–shell microsphere extraction coupled with liquid chromatography and triple quadrupole tandem mass spectrometry for the quantitative analysis of four plant growth regulators in navel oranges

“…Therefore, it is necessary to further develop appropriate enrichment and cleanup steps before the analysis to obtain accurate analytical data. At present, the separation and enrichment of PGRs in food include liquid–liquid microextraction , SPE , matrix dispersion extraction , etc. In these methods, the disadvantage of LLE and SPE is that the reagent dosage is too large, the separation efficiency is low, and the operation is relatively tedious.…”