Simultaneous Determination of Seven Synthetic Colorants in Wine by Dispersive Micro-Solid-Phase Extraction Coupled with Reversed-Phase High-Performance Liquid Chromatography

Abstract

A novel and effective dispersive micro-solid-phase extraction (d-µ-SPE) using ethanediamine-functionalized magnetic Fe3O4 polymer (EDA-MP) as an efficient adsorbent in wine sample was developed. Based on this, a simple and time-saving analytical method for the simultaneous determination of seven synthetic colorants (i.e., tartrazine, amaranth, carmine, sunset yellow, allura red, brilliant blue and erythrosine) in wine by reversed-phase high-performance liquid chromatography with an ultraviolet detector was established. The experimental parameters, including the chromatographic retention behavior of studied synthetic colorants, the effect of the usage amount of cross-linking monomer, the effect of the usage amount of EDA-MP on the recovery and the recyclability of the adsorbents, were studied in detail. The results showed that the EDA-MP could be reused efficiently at least six times. Under optimized conditions, the recoveries for all analytes were in the range of 88.6-105.2%, with the intraday relative standard deviations (RSDs) ranging from 2.1 to 8.2% and the interday RSDs ranging from 3.4 to 8.7%, and all the analytes had good linearities in the tested ranges with correlation coefficients (r(2)) >0.9995. The limits of quantification for seven synthetic colorants were between 0.12 and 0.45 mg L(-1). The developed method was successfully applied to wine samples, and it was confirmed that the EDA-MP particles were highly effective d-µ-SPE materials.

A Comparative Study of Advanced Stationary Phases for Fast Liquid Chromatography Separation of Synthetic Food Colorants

“…Applying fast chromatography approach of advanced technology of column packing material provided reduction of back pressure and fastening the separation. We achieved significant shortening of analysis of eight colorants in comparison with previously published methods [17,18,19,20,23]. One work published separation of eight colorants using core-shell column in time less than six min [29].…”

“…After appropriate pre-extraction and clean-up, it is a very common and versatile method for simultaneous determination of numerous colourings in complex food matrices. Despite of some recent interesting applications [16], thin-layer chromatography has been almost superseded by liquid chromatography (HPLC-DAD) [17,18,19,20,21,22,23], similarly very fast and simple method of sequential injection chromatography has been reported [24]. Nowadays, in many cases mass spectrometry detection with mass confirmation has been involved [25,26].…”

Adsorption of Food Coloring Allura Red Dye (E129) From Aqueous Solutions Using Activated Carbon

“…Therefore, the removal of the Allura red dye from aqueous solutions has a great importance. E 129 dye adsorption onto different adsorbents was reported using different analytical techniques such as potentiodynamic polarization (Peme et al, 2015), spectrophotometry (Bişgin et al, 2015, Zalacain et al, 2005, electrochemical methods (Alghamdi, 2005), HPLC (Zhang, 2015) and Fourier transform-infrared spectroscopic (FT-IR) analysis (Liang et al, 2015). Among these techniques, spectrophotometry stands out, as the most effective and convenient analytical techniques as compared to the others owing to its simplicity, low cost, and wide availability in most laboratories.…”

Chitosan-based adsorption and freeze deproteinization: Improved extraction and purification of synthetic colorants from protein-rich food samples

“…To compare the purification effect of chitosan with traditional polyamine, we examined the result after polyamine purification according to the reported method with minor revision (Zhang, Zhou, Wang, Wu, & Zhao, 2014). The extract obtained after deproteinization was concentrated in boiling water, and the removal of ammonia was carried out in parallel.…”