Simultaneous Determination of Seven Neuroactive Steroids Associated with Depression in Rat Plasma and Brain by High Performance Liquid Chromatography?Tandem Mass Spectrometry

Simultaneous Determination of Selected Steroids with Neuroactive Effects in Human Serum by Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry

“…However, it is recommended to use the matrix surrogate calibration curve for the analysis of metabolites in biological samples. 86 Compared to many available studies, LLOQ values for T, 34,39,47,81,82,85 DHEA, 35,39,41,45,47,81,82,85 PROG, 35,39,41,44,47 PREG, 39,47 ANDRO, 39,41,81,82,85 ALLO, 88,89 and DHT 39 were better or at least within 1 order of magnitude. Lower quantification limits for some substances in other methods may be due to the use of different ionization techniques.…”

“…42,44,51,57 NASs are studied by LC−MS/MS in various types of mammalian biological matrices such as plasma or serum, 13,22,34,41,43,47−49 the cerebrospinal fluid, 13,31,43 and brain tissues. 47,50,51 However, others can also be used: urine, 52,53 saliva, 42,54,55 hair, 46,56 or nails. 57 LC−MS/MSbased methods have been successfully used to determine selected representatives of androgens, estrogens, progestins, and corticosteroids.…”

“…For more than half a century, gas chromatography combined with mass spectrometry (GC–MS) has been used for the analysis of steroid substances . GC-based techniques using single MS or tandem mass spectrometry (MS/MS) are available. ,− Approaches to the steroid analysis have been enriched in recent years in particular by the use of liquid chromatography (LC). ,,,− The LC–MS/MS techniques offer several advantages over GC–MS, such as the high-throughput analysis (more suitable for a large set of samples), less time-consuming and generally easier sample preparation (usually, no derivatization is required), or the ability to quantify intact steroid conjugates (sulfates or glucuronides) . These properties make LC–MS/MS more suitable for routine use in clinical laboratories.…”

“…The chemical structures of the individual analytes and their physicochemical properties are shown in Figure and Table S1, respectively. These analytes are very important representatives of female and male steroid hormones and have been also studied in other NAS-focused research. − ,, The developed method combines a very simple and rapid extraction process with sensitive detection and quantification by ultrahigh-performance liquid chromatography with electrospray tandem mass spectrometry (UHPLC–ESI–MS/MS). To the best of our knowledge, no rapid and high-throughput comprehensive method is currently available for the simultaneously targeted profiling of a selected set of steroids with neuroactive effects in human serum.…”

Analytical Methods for the Determination of Neuroactive Steroids

“…For example, pregnenolone, progesterone, and 5α-dihydrotestosterone levels were increased, and in 5α-dihydroprogesterone and allopregnanolone, levels were decreased in patients with multiple sclerosis compared to the control group. In another study, chromatographic separation of the samples performed on a PR-C18 column was combined with an MS/MS analyzer using the positive mode of APCI and SRM transitions [109]. A simple and specific method has been developed for simultaneous determination of selected NASs, namely cortisone, cortisol, DHEA, estradiol, progesterone, pregnenolone, and testosterone.…”