Simultaneous determination of serum and urinary hydroxyproline and proline by liquid chromatography using two fluorescent labeling reagents

Kinetic study of paracetamol on prolidase activity in erythrocytes by capillary electrophoresis with Ru(bpy)32+ electrochemiluminescence detection

“…Although proline can be detected by UV or fluorescence detection [14,15], the derivatization process of proline limited the application of these methods, especially in bioanalysis. CE-ECL has been successfully applied to the detection of underivatized proline by many groups [16][17][18], and proved to be a relatively simple and sensitive method.…”

Separation and Simultaneous Determination of Proline and Enoxacin in Human Urine by Capillary Electrophoresis With Electrochemiluminescence

“…Proline is the predominant amino acid in the grape contents of proline vary in association with various diseases such as bone diseases, tumors and chronicuremia. [2] The widespread use of these compounds for clinical and pharmacological study requires to establish a fast and sensitive analytical technique for detection of the drugs in several biological fluids.…”

A rapid capillary electrophoresis with electrochemiluminescence method for the assay of human urinary proline and hydroxyproline

“…Hyp, formed by hydroxylation of Pro during collagen biosynthesis, is released after the degradation of collagen and excreted into urine (1). Hyp and Pro are present in free, peptide and protein forms in body fluids.…”

“…In addition, Pro and Hyp in urine have been used as important biochemical markers for human collagen metabolism. Many methods have been reported for the determination of Hyp alone or together with Pro, including HPLC with ultraviolet (UV) detection (6) or fluorescence detection (1,7), and GC with mass spectrometric detection (8,9). Nevertheless, HPLC and GC have some shortcomings, such as low resolution.…”