Simultaneous determination of salbutamol and terbutaline at overdose levels in human plasma by high performance liquid chromatography with electrochemical detection

Abstract

A multidimensional column chromatographic method involving electrochemical detection using a carbon fibre microelectrode flow cell was optimized and successfully applied to the simultaneous determination of salbutamol and terbutaline in plasma at overdose levels. This method performs, in a single step, an efficient extraction and clean-up of salbutamol and terbutaline from human plasma. The calibration graphs over three days were linear over the calibration range 20-100 ng/mL plasma with a limit of detection of 1 ng and 0.8 ng/mL plasma for salbutamol and terbutaline, respectively. The intra- and inter-assay coefficients of variation were less than 8% and the recoveries ranged from 94 to 96%. The accuracy of the assay, which was defined as the percentage difference between the mean concentration found and the theoretical concentration, was 7% or better. The proposed method combines the advantages of being simple, reproducible and selective in the presence of other sympathomimetic and commonly ingested drugs and is suitable for routine analyses to obtain valuable information about the clinical effects and treatment of overdose with these drugs. The whole procedure takes ca. 10 min and compares favourably with detection at a conventional glassy carbon electrode.

Ultra-sensitive determination of Formoterol in human serum by high performance liquid chromatography and electrospray tandem mass spectrometry

“…neither plasma nor urine, Fenoterol was determined in plasma after derivatisation with HPLC-fluorescence [9] to a LLOQ of 376 pg/mL or HPLC-MS/MS to a LLOQ of 2.5 ng/mL, Salbutamol (Albuterol) was determined with HPLC-MS [10] with a LLOQ of 100 pg/mL or GC-MS [11] with a LLOQ of 2 ng/mL or HPLC-fluorescence [12,13] with a LLOQ of 50 pg/mL, Terbutaline was determined with HPLC-MS/MS [14] with a LLOQ of 1 ng/mL or HPLC electrochemical detection [15] with a LLOQ of 0.8 ng/mL. All published methods regarding Formoterol related substances have a LLOQ between 50 pg/mL and 5 ng/mL in plasma or urine except Formoterol itself for which a LLOQ from 4 to 20 pg/mL was achieved with very time consuming methods [1,2].…”

Stability indicating HPTLC method for determination of terbutaline Sulfate in bulk and from Submicronized dry Powder inhalers

“…1,2 A literature survey revealed that various analytical methods have been reported for the estimation of TBS; they include UV spectroscopy, 3,4 voltammetry, 5,6 capillary electrophoresis, [7][8][9] chemiluminescence, [10][11][12] high performance liquid chromatography (HPLC), 1,13,14 HPLC-fluorescence detection, 15 HPLC-electrochemical detection, 16,17 HPLCchemiluminescence detection, 18 RP-liquid chromatography, 19 LC-electrochemical detection, 20 column-switching LC, 21 LC-tandem mass spectrometry, 22,23 and high-resolution GC-mass spectrometry analysis. 24 For TBS pharmacokinetic studies, the most useful analytical method is GC-mass spectrometry.…”

Determination of the enantiomers of salbutamol and its 4-O-sulphate metabolites in biological matrices by chiral liquid chromatography tandem mass spectrometry

“…7,8 Both normal phase, 9 but predominantly reversed phase, high performance liquid chromatography (HPLC) methods have been reported for salbutamol using either fluorescence 9,10,11,12 or electrochemical detection. 13,14,15 These HPLC methods have employed either liquid-liquid 11,13 or solid phase extraction (SPE) 9,12,15 procedures and the use of column switching technology has also been reported. 14 The main drawback of these HPLC methods has been a lack of sensitivity.…”

“…13,14,15 These HPLC methods have employed either liquid-liquid 11,13 or solid phase extraction (SPE) 9,12,15 procedures and the use of column switching technology has also been reported. 14 The main drawback of these HPLC methods has been a lack of sensitivity. With lower limits of quantification (LLoQ) in the low ng/mL range, they have only been suitable for defining the pharmacokinetics of salbutamol after oral and intravenous dosing.…”