Simultaneous determination of rosuvastatin and fenofibric acid in human plasma by LC–MS/MS with electrospray ionization: Assay development, validation and application to a clinical study

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

“…An addition of hidrocloric acid to the plasma samples afore the extraction procedure was shown to be necessary in order to obtain the non-ionized form of analyte which considerably improved extraction efficacy. Assays employing LLE with ethyl acetate [78] and ethyl ether [79] as extraction solvents for determination of rosuvastatin in plasma samples were already published with extraction recoveries of 74 and 69%, respectively. However, in the preliminary study by Lan and co-workers [80], it was found that the extraction recovery of rosuvastatin from plasma in most common organic solvents, such as above mentioned ethyl acetate and ethyl ether, was less than 20%, resulting in an insufficient, imprecise and inaccurate extraction procedure.…”

A Novel Method for Determination of Fenofibric Acid in Human Plasma using HPLC-UV: Application to a Pharmacokinetic Study of New Formulations

“…Absorbance (AU) Time (min) to previously reported methods [21][22][23][24][25]. This method gives narrow peaks, satisfactory selectivity and a short run-time as compared to the published methods.…”

“…necessary to develop a method for its determination in plasma. There have been several papers on the determination of FBT or its metabolites in biological fluids using techniques such as HPLC, GC-MS and LC-MS [7,17,[21][22][23][24][25][26]. Our method uses a simple one step liquid/liquid extraction procedure and HPLC-UV detection with a novel internal standard of 4'-chloro-5-fluro-2-hydroxybenzophenone (CFHB), (Figure 1c).…”

Simultaneous quantitation of rosuvastatin and gemfibrozil in human plasma by high-performance liquid chromatography and its application to a pharmacokinetic study

“…Several bioanalytical methods (HPLC and LC-MS/MS) exist for quantitation of GFZ (Hengy and Kolle, 1985;Forland et al, 1987;Knauf et al, 1990;Nakagawa et al, 1991;Sallustio and Fairchild, 1995;Benkone Markus 1996;Dix et al, 1999;Hermening et al, 2000;Gonzalez-Penas et al, 2001;Kim et al, 2003;Borges et al, 2005). To date there have only been two methods reported for the estimation of RST in human plasma, which utilizes highperformance liquid chromatography coupled with mass spectrometry (Hull et al, 2002;Trivedi et al, 2005). Of late, we have reported the method validation for RST in rat plasma using HPLC (Kumar et al, 2006).…”