Simultaneous determination of ribavirin and ribavirin base in monkey plasma by high performance liquid chromatography with tandem mass spectrometry

Measurement of ribavirin and evaluation of its stability in human plasma by high-performance liquid chromatography with UV detection

“…Moreover, no evidence of ribavirin decomposition was found during short-term (24 h) storage of plasma samples at room temperature and storage at −20 • C for 1 month (Table 4). This confirms the good stability of ribavirin in plasma both at room temperature and at −20 • C reported in previous studies [17,21,23,24].…”

“…In addition, discrepant data have been reported on stability of ribavirin following extraction from plasma. In a recent study [8], storage of dried extracts at −20 • C for 3 days was shown to induce a significant (37%) loss of ribavirin, whereas other authors reported that the ribavirin concentration remains stable in processed samples kept at 2-15 • C up to 52 h [21,24]. These discrepancies put the stability of ribavirin in question, and demanded further investigation.…”

“…Moreover, some debate exists about ribavirin stability under conditions that clinical samples commonly experience. Indeed, Larrat et al [8] reported a gradual decrease in the ribavirin concentration up to 33% in samples undergoing three freeze-thaw cycles, while no degradation of ribavirin in plasma subjected to repeated freeze-thaw cycles was observed in other studies [21][22][23][24]. In addition, discrepant data have been reported on stability of ribavirin following extraction from plasma.…”

“…Several analytical methods for quantitating ribavirin in serum or plasma have been developed; these include bioassay [13], radioimmunoassay [14], gas chromatography-mass spectroscopy [15], capillary electrophoresis (CE) [16], and highperformance liquid chromatography (HPLC) with UV [8,[16][17][18][19][20] or mass spectrometric (MS) [21][22][23][24] detection. However, a number of these methods require employment of radioactive reagents, gradient elution runs, and CE or MS instrumentation, which is not always available in standard hospital laboratories.…”

A sensitive LC–MS/MS method for quantification of a nucleoside analog in plasma: Application to in vivo rat pharmacokinetic studies

“…this generates LC-MS/MS methods that are not suitable for achieving fast bio-analysis. New types of LC-columns that can tolerate high amount of aqueous in the mobile phase (up to 100% aqueous phase) can offer an alternative for the separation of NA [11]. However, aqueous mobile phase has been speculated to yield poor ionization efficiency when using MS detection.…”