Simultaneous determination of retinol, β-carotene and α-tocopherol in adipose tissue by high-performance liquid chromatography

Penetration of cefuroxime in subcutaneous tissue during coronary artery bypass grafting surgery

“…Since no biological material from patients could be used for the development and optimization of the analytical method, in the present investigation subcutaneous tissue from dogs was employed based on the study of Casal et al [20], who used biological materials obtained from birds and pigs for the development and validation of a method for the quantification of vitamins in human subcutaneous tissue. Precision (CV%) Within-day 100 g/g 4.9% 10 g/g 5.5% 0.5 g/g 8.9%…”

“…Cefuroxime calibration standards were prepared in the laboratory as described previously by Casal et al [20], using subcutaneous tissue fragments obtained from dogs provided by the Animal House of Medical School (FMUSP) and not treated with the drug. The animals were previously anesthetized at the Laboratory of Experimental Anesthesia, Department of Anesthesiology, LIM -FMUSP, and subcutaneous tissue samples were collected from the chest region.…”

Iodine enrichment of rainbow trout flesh by dietary supplementation with the red seaweed Gracilaria vermiculophylla

“…The extraction of total lipids followed the method described by Folch et al (1957) with dichloromethane-methanol (2:1) and gravimetric determination. Vitamin E extraction followed methodologies previously described by Alves et al (2009a) and Casal et al (2001) with some modifications. Muscle samples (~1 g) were extracted with 5 mL of absolute ethanol and 10 mL of n-hexane (0.01% of BHT); 10 μL of an internal standard, tocol (2-methyl-2-(4,8,12-trimethyltridecyl)chroman-6-ol) (Matreya Inc., PA, USA), was also added.…”

Antioxidants As Analytes in Analytical Chemistry

“…Normal-phase HPLC was performed on a Lichrosorb Si60 column with gradient elution with a mixture of n-hexane and 2-propanol at a flow rate of 1 mL/min. A diode array system was used for detection in the case of retinol and β-carotene, and a fluorescence detector was used in the case of α-tocopherol [95].…”