Simultaneous Determination of Reserpine, Yohimbine and Ajmalicine in the Extract of Rauvolfia Rootstocks, Branches and Leaves by HPLC

Du, Yong; Feng, Ming; Wang, Yinan; Chen, Lin; Wang, Miao; Zhao, Chunjie

doi:10.14233/ajchem.2014.16584

Cited by 2 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RES and AJM were quantified by a novel method developed and validated for RP-HPLC using an UV detector. This method is rapid and precise as they give reliable elution at early retention time using organic mobile phase as compared to previously reported methods [21,22]. The RP-HPLC method used in the current study has many advantages as compared to previously reported methods.…”

Section: Discussionmentioning

confidence: 79%

Ajmalicine and Reserpine: Indole Alkaloids as Multi-Target Directed Ligands Towards Factors Implicated in Alzheimer’s Disease

et al. 2020

View full text Add to dashboard Cite

Alzheimer’s disease (AD) is a multifactorial disorder characterized by exponential loss of memory and cognitive deficit involving several disease modifying targets (amyloid beta, beta-secretase, monoaminoxidase-B, and cholinesterase). The present study explores multi-target directed ligand approach using secondary metabolite reserpine (RES) and ajmalicine (AJM) obtained from Rauwolfia serpentina roots. Novel LCMS and HPLC methods were developed for identification and quantification of reserpine and ajmalicine. In vitro enzyme inhibition assays were performed to evaluate anti-cholinesterase, β-site amyloid cleaving enzyme (BACE-1) inhibition and monoamine oxidase-B (MAO-B) inhibition, further analyzed with in silico analysis. Anti-amyloidogenic potential was studied using anti-aggregation studies along with TEM and circular dichroism (CD) analysis. In vitro neuroprotective potential against Aβ toxicity and anti-oxidative stress was demonstrated using PC12 cell cultures. Reserpine is a more potent dual cholinesterase inhibitor than ajmalicine (IC50 values of 1.7 μM (AChE) and 2.8 μM (BuChE)). The anti-aggregation activity of reserpine (68%) was more than ajmalicine (56%). Both compounds demonstrated neuroprotective activity against Aβ42 (92%) and H2O2 (93%) induced toxicity in PC12 cells against controls. Phytocompounds also inhibited MAO-B and BACE-1 enzymes in concentration dependent manner. Molecular docking studies indicated the strong binding of compounds to the catalytic site of targets. This novel study demonstrated that reserpine and ajmalicine as a multi-target directed ligand that have disease modifying potential for amelioration of AD.

show abstract

Section: Discussionmentioning

confidence: 79%

Ajmalicine and Reserpine: Indole Alkaloids as Multi-Target Directed Ligands Towards Factors Implicated in Alzheimer’s Disease

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Some indole derivatives show anticarcinogenic, hypotensive and antineoplastic activities (Zhang et al, 2015). The indole skeleton can be found in many bioactive drugs, such as ajmalicine (Du et al, 2014), vinblastine (Ishikawa et al, 2008) and reserpine (Chen & Huang, 2005).…”

Section: Chemical Contextmentioning

confidence: 99%

Synthesis and structure of 1-(2-bromophenyl)-2-chloro-3-(2-chloracetyl)-1H-indole

et al. 2016

View full text Add to dashboard Cite

In this indole derivative, the dihedral angle between the mean plane of the indole ring system and the mean plane of the benzene ring of the 2-bromophenyl group is 77.6 (1)°. In the crystal, pairs of molecules are face-to-face embraced via two weak C—H⋯O hydrogen bonds, forming inversion dimers which are connected by head-to-head Cl⋯Cl intermolecular contacts to build a molecular sheet parallel to (101). Neighbouring sheets are stacked together by further short Cl⋯Cl intermolecular contacts to construct the three-dimensional structure.

show abstract

Simultaneous Determination of Reserpine, Yohimbine and Ajmalicine in the Extract of Rauvolfia Rootstocks, Branches and Leaves by HPLC

Cited by 2 publications

References 4 publications

Ajmalicine and Reserpine: Indole Alkaloids as Multi-Target Directed Ligands Towards Factors Implicated in Alzheimer’s Disease

Ajmalicine and Reserpine: Indole Alkaloids as Multi-Target Directed Ligands Towards Factors Implicated in Alzheimer’s Disease

Synthesis and structure of 1-(2-bromophenyl)-2-chloro-3-(2-chloracetyl)-1H-indole

Contact Info

Product

Resources

About