Simultaneous Determination of Pyridate, Quizalofop-ethyl, and Cyhalofop-butyl Residues in Agricultural Products Using Liquid Chromatography-Tandem Mass Spectrometry

Abstract

An analytical method was developed to simultaneously determine pyridate, quizalofop-ethyl, and cyhalofop-butyl in brown rice, soybean, potato, pepper, and mandarin using LC-MS/MS. Purification was optimized using various sorbents: primary–secondary amine, octadecyl (C18) silica gel, graphitized carbon black, zirconium dioxide-modified silica particles, zirconium dioxide-modified silica particles (Z-SEP), and multi-walled carbon nanotubes (MWCNTs). Three versions of QuECHERS methods were then tested using the optimal purification agent. Finally, samples were extracted using acetonitrile and QuEChERS EN salts and purified using the Z-SEP sorbent. A six-point matrix-matched external calibration curve was constructed for the analytes. Good linearity was achieved with a determination coefficient ≥0.999. The limits of detection and quantification were 0.0075 mg/kg and 0.01 mg/kg, respectively. The method was validated after fortifying the target standards to the blank matrices at three concentration levels with five replicates for each concentration. The average recovery was within an acceptable range (70–120%), with a relative standard deviation <20%. The applicability of the developed method was evaluated with real-world market samples, all of which tested negative for these three herbicide residues. Therefore, this method can be used for the routine analysis of pyridate, quizalofop-ethyl, and cyhalofop-butyl in agricultural products.

A new multiresidue thin layer chromatography–ultraviolet detection method for the rapid determination of six herbicides residue applied as ready mix formulation for rice

“…Shim et al. [42] reported 0.0075 and 0.01 mg/kg LOD and LOQ by LC/MS/MS for cyhalofop in brown rice. Xia et al.…”

A Comprehensive Review of Pesticide Residues in Peppers

“…Gas chromatography (GC), high-performance liquid chromatography (HPLC), and chromatography−mass spectrometry (GC-MS) are among the main methods that are used for pesticide residue detection and metabolite detection [11,28]. These conventional detection methods have good sensitivity, accuracy, precision, and reliability.…”

“…The quantity and safety of agricultural products are intimately linked to public health, social stability, and sustainable development. Therefore, this topic has gained increasing concern among the general public, health authorities, and the scientific community [11]. The World Health Organization (WHO) states that fruits and vegetables are crucial to a healthy diet.…”