Simultaneous determination of pseudouridine, neopterine and creatinine in urine by ion-pair high-performance liquid chromatography with in-series ultraviolet and fluorescence detection

Palmisano, Francesco; Rotunno, T.; Sorsa, Massimo La; Zambonin, Carlo; Abbate, I.

doi:10.1039/an9952002185

Cited by 11 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Urine creatinine was used to normalize the urine concentrations of enrofloxacin and ciprofloxacin (Palmisano et al, 1995). This approach eliminates the problem of contamination with drinking water.…”

Section: Methodsmentioning

confidence: 99%

Influence of PorcineActinobacillus pleuropneumoniaeInfection and Dexamethasone on the Pharmacokinetic Parameters of Enrofloxacin

Post

Cope²,

Farrell³

et al. 2002

J Pharmacol Exp Ther

View full text Add to dashboard Cite

The impact of Actinobacillus pleuropneumoniae (APP) infection in swine on the pharmacokinetic parameters of enrofloxacin were determined. Twenty-four animals were used in a 2 ϫ 2 factorial of treatment groups (six animals per group) to determine the impact of APP-induced inflammation and the antiinflammatory drug dexamethasone on enrofloxacin pharmacokinetic parameters. All animals received enrofloxacin as a single intravenous dose (5 mg/kg). Administration of dexamethasone was associated with an increase in clearance of enrofloxacin Clearance of enrofloxacin was not affected by APP. Volume of distribution at steady state was significantly increased in the dexamethasone-treated pigs. Volume of distribution at steady state was decreased by APP infection. Dexamethasone significantly increased the terminal elimination half-life of enrofloxacin. APP infection decreased the terminal elimination half-life of enrofloxacin in the infected pigs. Infection and dexamethasone significantly decreased the urine enrofloxacin/creatinine and ciprofloxacin/creatinine ratios. This study shows that APP infection does affect plasma pharmacokinetic parameters. Dexamethasone and APP infection may reduce renal clearance of enrofloxacin with a compensatory increase in intestinal clearance. Neither infection nor dexamethasone altered the metabolism of enrofloxacin to ciprofloxacin, the principal metabolite of enrofloxacin.

show abstract

Section: Methodsmentioning

confidence: 99%

Influence of PorcineActinobacillus pleuropneumoniaeInfection and Dexamethasone on the Pharmacokinetic Parameters of Enrofloxacin

Post

Cope²,

Farrell³

et al. 2002

J Pharmacol Exp Ther

View full text Add to dashboard Cite

show abstract

“…[15][16][17] The role of modified nucleosides as tumour markers is widely recognised. [18][19][20][21] Increased levels of 5-methyl-2'-deoxycytidine (m 5 dCyd), a modified nucleoside probably derived from DNA, were recently detected in urine of leukaemia patients, for the first time, by an inhibition ELISA method. 22 Thus m 5 dCyd was proposed to be a new marker for the leukaemia disease.…”

mentioning

confidence: 99%

Electrospray ionization mass spectrometry of 5-methyl-2′-deoxycytidine and its determination in urine by liquid chromatography/electrospray ionization tandem mass spectrometry

Zambonin

Palmisano

1999

Rapid Commun. Mass Spectrom.

Self Cite

View full text Add to dashboard Cite

The behaviour of 5-methyl-2'-deoxycytidine (m(5)dCyd, claimed to be a potential marker for leukaemia) during the electrospray process was studied. In particular, considerations concerning the effect of solution chemistry (e.g. analyte concentration, pH, etc.) on electrospray ionization mass spectra were drawn. Furthermore, a procedure using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) for the determination of urinary m(5)dCyd is described. The method is simple, sensitive and highly specific. The pre-treatment procedure gave an average recovery of 79% (relative standard deviation (RSD) of 3%). Method performance was evaluated on spiked urine samples covering the concentration range from 50 ng/mL to 10 microgram/mL, the same as that of an existing inhibition ELISA method. Contrary to findings based on this immunoassay technique, urinary m(5)dCyd in healthy individuals was not detectable and did not increase in the presence of the malignant disease.

show abstract

“…[1][2][3][4][5] Various methods have been developed for quantifying modified nucleosides, based on an enzyme-linked immunosorbent assay (ELISA), 6,7 32 P-post-labelling, 8 capillary electrophoresis, 9,10 HPLC with photodiode array detection 11,12 or ECD, 13 and GC/MS. [14][15][16] However, GC/MS analysis faced additional problems in the hydrolysis and derivatization steps.…”

mentioning

confidence: 99%

A rapid and sensitive method for quantitation of nucleosides in human urine using liquid chromatography/mass spectrometry with direct urine injection

Lee

Jung

Kim

et al. 2004

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Oxidized nucleosides are biochemical markers for tumors, aging, and neurodegenerative diseases. However, during the last decade, the analytical methods for nucleosides by gas chromatography/ mass spectrometry (GC/MS) and high-performance liquid chromatography (HPLC) with singleparameter detectors like electron-capture detection (ECD) have not been sufficiently rapid or reliable to detect nucleosides in urine and to analyze clinical samples. It has been reported (Dudley et al., Rapid Commun Mass Spectrom. 2000; 14: 1200) that liquid chromatography with electrospray mass spectrometry (LC/ESI-MS) is more specific and sensitive for analysis of nucleosides than HPLC with conventional detectors; however, this method required complex extraction steps. In the present work a direct LC/ESI-MS method for nucleosides without extraction of urine samples has been developed. Analysis of nucleosides using positive-ion mode with selected reaction monitoring effectively eliminated potential interferences from endogenous constituents of the urine. This highly selective and sensitive method made it possible to analyze urinary nucleosides with a lower limit of quantitation of 0.2 nmol/mL. The method has been validated, with both excellent linearity and reproducibility, in the calibration range from 0.2-400 nmol/mL. The correlation coefficients of the calibration curves were higher than 0.987. The coefficients of variation were in the range 0.03-14.92% (inter-day) and 0.54-14.39% (intra-day), respectively. Copyright # 2004 John Wiley & Sons, Ltd.Oxidized nucleosides represent excellent biomarkers for determining the extent of damage in genetic material, which has long been of interest in understanding the mechanism of aging, neurodegenerative diseases, and carcinogenesis. [1][2][3][4][5] Various methods have been developed for quantifying modified nucleosides, based on an enzyme-linked immunosorbent assay (ELISA), 6,7 32 P-post-labelling, 8 capillary electrophoresis, 9,10 HPLC with photodiode array detection 11,12 or ECD, 13 and GC/MS. 14-16 However, GC/MS analysis faced additional problems in the hydrolysis and derivatization steps. 17 HPLC/ECD analysis has limitations in that the requirement for electroactive components meant that the method was mainly applicable to the detection of only 8-oxodeoxyguanosine. 18 Due to their chemical characteristics in HPLC analysis, various kinds of nucleosides could not be analyzed using just one kind of detector.To overcome these problems, in the present work, we developed an analytical method for determining many kinds of nucleosides at one time using LC/MS with ESI (electrospray ionization) detection. We confirmed that mass spectrometric detection with ESI is more specific and sensitive than any other HPLC detection method. We also developed a direct analytical method for nucleosides without extracting urine samples. This approach is both more convenient and more efficient in conducting studies with large sample numbers, e.g., a clinical study, than analytical methods involving complex extrac...

show abstract

Simultaneous determination of pseudouridine, neopterine and creatinine in urine by ion-pair high-performance liquid chromatography with in-series ultraviolet and fluorescence detection

Cited by 11 publications

References 9 publications

Influence of PorcineActinobacillus pleuropneumoniaeInfection and Dexamethasone on the Pharmacokinetic Parameters of Enrofloxacin

Influence of PorcineActinobacillus pleuropneumoniaeInfection and Dexamethasone on the Pharmacokinetic Parameters of Enrofloxacin

Electrospray ionization mass spectrometry of 5-methyl-2′-deoxycytidine and its determination in urine by liquid chromatography/electrospray ionization tandem mass spectrometry

A rapid and sensitive method for quantitation of nucleosides in human urine using liquid chromatography/mass spectrometry with direct urine injection

Contact Info

Product

Resources

About