Simultaneous determination of piroxicam and norfloxacin in biological fluids by high‐performance liquid chromatography with fluorescence detection at zero‐order emission mode

Abstract

A new highly sensitive high-performance liquid chromatographic method with fluorescence detection (HPLC-FLD) in zero-order emission mode was developed for the first time for the simultaneous determination of piroxicam (PRX) and norfloxacin (NRF) in biological fluids. The fluorescence detector wavelengths were set at 278 nm for excitation and zero-order mode for emission. The zero-order emission mode produced greater sensitivity for the measurement of both drugs than a fixed emission wavelength (446 nm). The new developed method was validated according to International Conference of Harmonization (ICH) guidelines. Linearity was found to be over concentration ranges 0.001-20 μg/ml and 0.00003-0.035 μg/ml for PRX and NRF, respectively. The limits of detection were 4.87 × 10 −4 and 1.32 × 10 −5 μg/ml for PRX and NRF, and the limits of quantitation were 1.47 × 10 −3 and 4.01 × 10 −5 μg/ml, respectively. The current fluorescence method was found to be more sensitive than most commonly used analytical methods and was successfully applied for simultaneous determination of PRX and NRF in biological fluids (serum and urine) with recoveries ranging from 91.67% to 100.36% for PRX and from 96.00% to 101.43% for NRF.

LC–MS/MS approach for simultaneous assessment of hyoscine N‐butyl bromide and ketoprofen in biological fluids and pharmaceuticals

“…Remarkably, these methods were less sensitive and selective if compared with the LC–MS hyphenated technique as it is well known that MS detectors are more powerful and highly sensitive than UV detectors (Ali et al, 2013; Al‐Sanea & Gamal, 2022; Tuzimski & Petruczynik, 2020). For the separation and quantification of a mixture of drugs, HPLC is an extremely efficient technique, but it lacks the essential ultra‐sensitivity for detection in human biological fluids (Ali et al, 2022; Gamal, Ali, Abdelfatah, & Magdy, 2019; Gamal, Ali, Fraihat, & Seaf Elnasr, 2019; Gamal & Elhalim, 2020; Gamal, Naguib, & Abdelfatah, 2021). Moreover, these HPLC methods have not been tested in human biological fluids for therapeutic drug monitoring.…”

A CaCuSi4O10/GCE electrochemical sensor for detection of norfloxacin in pharmaceutical formulations

“…Methods such as liquid chromatography-tandem mass spectrometry (LC-MS), 8 fluorescence-spectrophotometry, 9 electro-chemiluminescence, 10 capillary electrophoresis, 11 high-performance liquid chromatography (HPLC) 12 and spectrofluorimetry 13 were used to analyse for the presence of norfloxacin. However, some of these methods are time-consuming and require laborious sample pre-treatment, large organic solvent consumption, expensive equipment, and advanced technical expertise.…”

Quantitative Analysis of Abamectin, Albendazole, Levamisole HCl and Closantel in Q-DRENCH Oral Suspension Using a Stability-Indicating HPLC-DAD Method

“…Therefore, the addition of ABA and/or CLO greatly increases animals’ protection from recurrent infection [ 3 ]. Simultaneous analysis methods for drug mixtures are strongly desired in daily analysis in QC entities in pharmaceutical firms and both governmental and private drug investigation centers [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ].…”