Simultaneous Determination of Phenolic Acids in Water Caltrop by HPLC-DAD

Stoicescu, Iuliana; Popescu, Antoanela; Sîrbu, Rodica; Bala, Camelia

doi:10.1080/00032719.2012.694943

Cited by 9 publications

(5 citation statements)

References 12 publications

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“…aqueous extract. In the alcoholic and aqueous extract, the hydrophilic compounds are determined, and in the etheric one, the lipophilic compounds [14][15][16][17]. The three solutions are analyzed separately, using methods corresponding to the physicochemical properties of each group of active principles [18].…”

Section: Physico-chemical Methodsmentioning

confidence: 99%

Valuable Bioactive Compounds Extracted from Ceramium rubrum on the Romanian Seaside with Medical Interest

Dragan

Sîrbu

Cadâr

2022

European Journal of Natural Sciences and Medicine

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Recently in science, seaweeds are widely used because of their relevant compounds and potential origin of antimicrobial, antiviral and antioxidant activity. Seaweeds are rich in the trace elements and essential minerals that are hard to find in terrestrial plants. Ceramium rubrum is one of the red seaweeds that can be found in the Black Sea on the Romanian coast that has not been enough studied for their bioactive compounds. Identification and quantification of the biomolecules is carried out by specific methods. The compounds that have been found are cumarines, sterols, flavonoic aglicoles, triterpenes and polysaccharides. Is well known that this kind of biomolecules are very important in the pharmaceutical industry. Ozes and poliozes, reducing compounds and catehic tanin we’re also identified from the red algae.

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Section: Physico-chemical Methodsmentioning

confidence: 99%

Valuable Bioactive Compounds Extracted from Ceramium rubrum on the Romanian Seaside with Medical Interest

Dragan

Sîrbu

Cadâr

2022

European Journal of Natural Sciences and Medicine

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“…The method was validated in terms of linearity, precision, accuracy, and specificity, limit of detection and limit of quantification [13].…”

Section: Hplc Analysis Of Phenolic Compoundsmentioning

confidence: 99%

Determination of caftaric acid in tincture and rose water obtained from Rosae damascenae flores

Popescu

Matei

Roncea

et al. 2015

Ovidius University Annals of Chemistry

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Polyphenolic compounds were determined from a pharmaceutical (tincture) and a cosmetic preparation (rose water), both obtained from the Rosae damascenae flores. Separation of the phenolic compounds was done by a HPLC method, using a Zorbax XDB or equivalent column C18, 250 mm x 4,6 mm; 5 μm. A gradient elution was performed with phosphoric acid and acetonitrile eluted under gradient conditions. The flow rate was 1.5 mL/min and the injection volume was 20 μL. HPLC method for determination of caftaric acid presented in this paper, has been validated. The results were statistically analyzed with SPSS 10 software.

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“…The extracts from Trapa bispinosa Roxb. shell contain many phenolic compounds, such as gallic acid, caffeic acid, naringin, and 1,2,3,4,6-pentagalacyl-β-D-glucose [ 4 , 5 , 6 ]. These phenolic compounds have specific physiological antioxidant, anti-inflammatory, and anticancer properties [ 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Expression, and Functional Analysis of Glycosyltransferase (TbUGGT) Gene from Trapa bispinosa Roxb.

Yin

Sun

et al. 2022

Molecules

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Trapa bispinosa Roxb. is an economical crop for medicine and food. Its roots, stems, leaves, and pulp have medicinal applications, and its shell is rich in active ingredients and is considered to have a high medicinal value. One of the main functional components of the Trapa bispinosa Roxb. shell is 1-galloyl-beta-D-glucose (βG), which can be used in medical treatment and is also an essential substrate for synthesizing the anticancer drug beta-penta-o-Galloyl-glucosen (PGG). Furthermore, gallate 1-beta-glucosyltransferase (EC 2.4.1.136) has been found to catalyze gallic acid (GA) and uridine diphosphate glucose (UDPG) to synthesize βG. In our previous study, significant differences in βG content were observed in different tissues of Trapa bispinosa Roxb. In this study, Trapa bispinosa Roxb. was used to clone 1500 bp of the UGGT gene, which was named TbUGGT, to encode 499 amino acids. According to the specificity of the endogenous expression of foreign genes in Escherichia coli, the adaptation codon of the cloned original genes was optimized for improved expression. Bioinformatic and phylogenetic tree analyses revealed the high homology of TbUGGT with squalene synthases from other plants. The TbUGGT gene was constructed into a PET-28a expression vector and then transferred into Escherichia coli Transsetta (DE3) for expression. The recombinant protein had a molecular weight of 55 kDa and was detected using SDS-PAGE. The proteins were purified using multiple fermentation cultures to simulate the intracellular environment, and a substrate was added for in vitro reaction. After the enzymatic reaction, the levels of βG in the product were analyzed using HPLC and LC-MS, indicating the catalytic activity of TbUGGT. The cloning and functional analysis of TbUGGT may lay the foundation for further study on the complete synthesis of βG in E. coli.

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Simultaneous Determination of Phenolic Acids in Water Caltrop by HPLC-DAD

Cited by 9 publications

References 12 publications

Valuable Bioactive Compounds Extracted from Ceramium rubrum on the Romanian Seaside with Medical Interest

Valuable Bioactive Compounds Extracted from Ceramium rubrum on the Romanian Seaside with Medical Interest

Determination of caftaric acid in tincture and rose water obtained from Rosae damascenae flores

Molecular Cloning, Expression, and Functional Analysis of Glycosyltransferase (TbUGGT) Gene from Trapa bispinosa Roxb.

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