Simultaneous Determination of Paracetamol, Chlorzoxazone and Diclofenac Sodium in Tablet Dosage Form by High Performance Liquid Chromatography

Abstract

A simple, precise and rapid isocratic reverse phase high performance liquid chromatographic method was developed for the simultaneous determination of paracetamol, chlorzoxazone and diclofenac sodium from tablet dosage form. The chromatographic separation was performed on an inertsil C18column (250 mm × 4.6 mm i.d 5 µm particle size). Mobile phase consisted of a mixture of phosphate buffer (0.02 M KH2PO4, pH adjusted to 3.7 using orthophosphoric acid), acetonitrile and methanol in the ratio of (25: 25: 50) at a flow rate of 1.0 mL/min. The wavelength was set at 220 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. The calibration was linear over the range of 50-150 µg/mL for paracetamol, 50-150 µg/mL for chlorzoxazone and 5-15 µg/mL for diclofenac sodium. The retention times were found as 2.8 min for paracetamol, 4.2 min for chlorzoxazone and 6.4 min for diclofenac sodium. The method can be easily adopted for quality control analysis.

Mefenamic Acid Selective Membranes Sensor and Its Application to pharmaceutical Analysis

“…Various methods have been reported for the determination of mefenamic acid as pure and in dosages forms. These methods include titrimetric [9,10], chromatographic [11][12], luminescence [13], flow injection [14][15], electrometric [16], spectroflourometric [17][18] and spectrophotometric methods [19][20][21][22].…”

Efficient processing of Single and Multiple Spectral Variables for Resolution and Quantitation of Paracetamol, Chlorzoxazone and Diclofenac

“…Literature review revealed that the three cited components were simultaneously determined in ternary mixture by HPLC, HPTLC [24][25][26][27][28][29] , and by simultaneous equation methods [30][31] . To our knowledge, chemometric techniques for simultaneous determination of these drugs have not been reported.…”

Simultaneous Spectrophotometric Determination of Diclofenac Sodium, Paracetamol, and Chlorzoxazone in Ternary Mixture Using Chemometric and Artificial Neural Networks Techniques

“…Other methods were developed for the simultaneous determination of DIC in binary combination with PAR or CHZ that include spectrophotometry [23][24][25], densitometry [26], voltammetry [27], and HPLC-UV [28,29]. Some methods were reported for the simultaneous determination of ternary combination of the three drugs based on densitometry [30,31] and HPLC-UV [32,33].…”