Simultaneous Determination of Oxolinic, Nalidixic and Piromidic Acids in Fishes by High Performance Liquid Chromatography

Kasuga, Yoji; Sugitani, Akira; Yamada, Fujizo

doi:10.3358/shokueishi.23.344

Cited by 20 publications

(4 citation statements)

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“…The frozen samples were homogenized and from the homogenate two samples were weighed out for analysis. OA was determined by the method of Kasuga, Sugitani & Yamada (1982) and Kasuga et al (1984). but with a 1000 mm column.…”

Section: Methodsmentioning

confidence: 99%

Withdrawal times of freshwater rainbow trout, Salmo gairdneri Richardson, after treatment with oxolinic acid, oxytetracycline and trimetoprim

Jacobsen¹

1989

Journal of Fish Diseases

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. Oxolinic acid (OA), oxytetracyline (OTC) and trimetoprim (TMP) were investigated in whole gutted rainbow trout, Salmo gairdneri Richardson, and in their skin, muscle and blood, at 6, 12 and 18°C. Concentrations of drugs were measured by high‐pressure liquid chromatography. It is recommended that withdrawal times for rainbow trout be based on whole gutted rainbow trout and not, as in earlier investigations, from residual concentrations in muscle. Any concentrations in rainbow trout feed were found to be stable during the pelleting process used and up to 81 days after pelleting. The absorption of the drugs in rainbow trout show different temperature dependencies. A quantitative comparison of our overall data from this work with individual results from earlier investigations was undertaken. Suggested withdrawal times for freshwater rainbow trout treated with these drugs are: for OTC, 90 days below 6°C, 70 days between 6 and 12°C and 60 days above 12°C; for TMP, 60 days below 8°C and 40 days above 8°C; for OA, 20 days at approximately 18°C.

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Section: Methodsmentioning

confidence: 99%

Withdrawal times of freshwater rainbow trout, Salmo gairdneri Richardson, after treatment with oxolinic acid, oxytetracycline and trimetoprim

Jacobsen¹

1989

Journal of Fish Diseases

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“…The extraction of OA and FLU from fresh ®sh muscle used organic solvents and manual work-up procedures including solid±liquid extraction (Kasuga et al 1982, Horii et al 1987, Samuelsen 1989, Thanh et al 1990, Carignan et al 1991, Hormazabal and Yndestad 1994, Munns et al 1995, 1998, Pfenning et al 1996, Ueno andAoki 1996) and/ or solid-phase extraction , Ikai et al 1989, BjoÈ rklund 1990, Plakas et al 1999. None of the published methods appeared to be eOE ective for freeze-dried muscle with attached skin (data not shown).…”

Section: Extraction and Recoveriesmentioning

confidence: 99%

“…Several methods have been published describing the determination of OA and FLU in ®sh muscle without attached skin (Kasuga et al 1982, Horii et al 1987, Ikai et al 1989, Samuelsen 1989, BjoÈ rklund 1990, Thanh et al 1990, Carignan et al 1991, Hormazabal and Yndestad 1994, Munns et al 1995, Pfenning et al 1996, Ueno and Aoki 1996, Munns et al 1998, Plakas et al 1999. However, the CVMP recommends the analyse of muscle with a naturally occurring amount of skin for drug residues.…”

Section: Introductionmentioning

confidence: 98%

Determination of residues of oxolinic acid and flumequine in freeze-dried salmon muscle and skin by HPLC with fluorescence detection

Pouliquen¹,

Morvan²

2002

Food Additives and Contaminants

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A procedure for the determination of residues of oxolinic acid (OA) and flumequine (FLU) in freeze-dried salmon muscle with attached skin, using reversed-phase high-performance liquid chromatography, is described. OA and FLU were extracted by a solid-liquid extraction procedure: after addition of hydrochloric acid, extraction used successively ethyl acetate, sodium hydroxide and chloroform. Liquid chromatography was performed on a 5 microm PuroSpher RP-18E cartridge using acetonitrile and 0.02 M aqueous orthophosphoric acid solution as mobile phase, with fluorescence detection. The performance of the method was established by spiking tissues with OA and FLU before the freeze-drying step. The method was linear over the concentration range 50-2000 ng/g freeze-dried tissue. Limits of detection and quantitation were 3.2 and 16 ng/g wet weight tissue respectively both for OA and FLU. Mean extraction recoveries of OA and FLU from freeze-dried tissue were 85.5 and 85.2% respectively. The method is suitable as a regulatory one for determination of residues of OA and FLU in freeze-dried salmon tissue.

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“…[6][7] Several methods such as fluorometry, 2 colorimetry, 6 high performance liquid chromatographic methods, [8][9][10][11] and gas chromatography 12 have been developed for the detection of the two compounds.…”

Section: Introductionmentioning

confidence: 99%

Sodium Borohydride and Hydrogen Peroxide as Fluorogenic Spray Reagents for the Detection of Nalidixic Acid and Flumequine

Wang

Chen

Kuo

1999

Journal of Liquid Chromatography & Related Technologies

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A simple, sensitive and highly selective detection method for nalidixic acid and flumequine has been developed. A 1-µL aliquot of sample solution was spotted onto the thin-layer plate with the aid of a disposable micropipette, and after development, the plate was sprayed first with sodium borohydride and then hydrogen peroxide successively. The spots were visualized under longwave UV light. The detection limits were 30 pmol for nalidixic acid and 60 pmol for flumequine. Downloaded by [Laurentian University] at 18:47 05 October 2014 ORDER REPRINTS 772 WANG ET AL.

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Simultaneous Determination of Oxolinic, Nalidixic and Piromidic Acids in Fishes by High Performance Liquid Chromatography

Cited by 20 publications

References 0 publications

Withdrawal times of freshwater rainbow trout, Salmo gairdneri Richardson, after treatment with oxolinic acid, oxytetracycline and trimetoprim

Withdrawal times of freshwater rainbow trout, Salmo gairdneri Richardson, after treatment with oxolinic acid, oxytetracycline and trimetoprim

Determination of residues of oxolinic acid and flumequine in freeze-dried salmon muscle and skin by HPLC with fluorescence detection

Sodium Borohydride and Hydrogen Peroxide as Fluorogenic Spray Reagents for the Detection of Nalidixic Acid and Flumequine

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