Simultaneous determination of nucleosides and nucleotides in dietary foods and beverages using ion-pairing liquid chromatography–electrospray ionization-mass spectrometry

Optimization of High Performance Liquid Chromatography Method for Simultaneous Determination of Some Purine and Pyrimidine Bases

“…The most widely used technique for biological samples is reversed phase (RP) or RP ion-pairing 15 high performance liquid chromatography (HPLC) because it gives reproducible results, and is sensitive, selective and easily automated. [2][3][5][6][7]10,[12][13][14] Capillary electrophoresis is also widely used for the separation of these analytes because of its minimal sample volume, short analysis time and high separation efficiency. [16][17][18] For both techniques UV/VIS, 5,18 electrochemical 19 or MS detection is used.…”

“…[16][17][18] For both techniques UV/VIS, 5,18 electrochemical 19 or MS detection is used. [13][14]18,[20][21][22] Since nucleobases are electroactive, in some papers voltammetric determination with static mercury drop electrode, by modified graphite/carbon electrodes, [23][24] and on the boron-doped carbon nanotubes are also reported. 27 In this study HPLC with isocratic elution with UV and MS detection was employed for the separation and determination of nine purine and pyrimidine bases.…”

Analysis of Intra- and Extracellular Levels of Purine Bases, Nucleosides, and Nucleotides in HepG2 Cells by High-performance Liquid Chromatography

“…16 These biochemicals have now been measured in a variety of systems, including cultured cells, [17][18][19] isolated tissues, 15 food substances, [12][13][14][20][21][22][23][24][25] fungi, 26 and biological samples. 11 Previously, we also reported the use of the HPLC method for determining the purine content of food, 14 beer, and beer-like alcoholic beverages, 12 whereas LC-MS was suitable for the simultaneous analysis of 23 species of purine bases, pyrimidines, nucleosides, and nucleotides.…”

“…11 Previously, we also reported the use of the HPLC method for determining the purine content of food, 14 beer, and beer-like alcoholic beverages, 12 whereas LC-MS was suitable for the simultaneous analysis of 23 species of purine bases, pyrimidines, nucleosides, and nucleotides. 24 However, similar to most methods described, the simultaneous determination of purine bases (A, G, HX, X, and UA), nucleosides [(adenosine (Ado), guanosine (Guo), inosine (Ino), xanthosine (Xao), deoxyadenosine (dAdo), deoxyguanosine (dGuo), and deoxyinosine (dIno)], and nucleotides [Adenosine 5′-triphosphate (ATP), adenosine 5′-diphosphate (ADP), AMP, guanosine 5′-triphosphate (GTP), guanosine 5′-diphosphate (GDP), GMP, IMP, and xanthosine 5′-monophosphate (XMP), nicotinamide adenine dinucleotide (NAD + )] has yet to be reported. A method for the determination of the full profiles of nucleotides and their metabolites (nucleosides and purine bases) in various samples would be valuable for physiological and pharmacological studies.…”

Electrocatalysis Oxidation of GMP Based on Layered Double Hydroxide Functionalized with Anionic Surfactant and Room Temperature Ionic Liquid Modified Glassy Carbon Electrode

“…In the past, different analytical methods have been studied for the analysis of GMP, based on high-performance liquid chromatography (HPLC), 4 liquid chromatography-mass spectrometry, [5][6][7][8] followed by spectrophotometric and radioisotopic detection. 9,10 Electrochemical method can also be used for the investigation of GMP.…”