Simultaneous Determination of Nitrite and Nitrate in Milk Samples by Ion Chromatography Method and Estimation of Dietary Intake

“…The widespread use of fertilizers, domestic, agricultural, and industrial wastes have increased the chances of nitrite and nitrate into manufactured dairy products [4].…”

Section: Introductionmentioning

confidence: 99%

“…Several analytical methods for the determination of nitrite and nitrate in different matrices have been reported [19]. These methods include ion chromatography (IC) [4,10,11], high performance liquid chromatography (HPLC) [6-8, 12, 13], differential pulse voltammetry [8], capillary electrophoresis [9] or others analytical method [19].…”

Section: Introductionmentioning

confidence: 99%

"Determination of nitrate and nitrite content in Zonar milk serum and derived dairy drinks using ion-pair reversed-phase high performance liquid chromatography "

Filip¹,

Prodan²,

Moldovan³

et al. 2019

Studia UBB Chemia

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The presence of nitrite and nitrate in milk and dairy products may cause serious health problem for consumers. Thus, a simple and accurate ion-pair reversed-phase high performance liquid chromatographic (IP-RP-HPLC) method has been developed and validated for determination of nitrite and nitrate in some dairy drinks based on Zonar Milk Serum. The separation was achieved on a Synergy Hydro-RP polar endcapped C18, 80Å, 4m (250  4.6 mm) column using isocratic elution with 0.01M n-octylamine and methanol, 90:10 (v/v) at pH=7; with flow rate of 1 mL•min-1 and detection at 214 nm and 25°C. A linear response (R 2 ≥ 0.9994) was observed in the range between 3.125 and 75 µg mL-1 for the nitrite and nitrate standard solutions. The limits of detection (LOD) and quantification (LOQ) were found as 0.25 µg mL-1 and 0.8 µg mL-1 for nitrite and 0.4 µg mL-1 and 1.2 µg mL-1 for nitrate, respectively. The method was validated according to ICH guidelines with respect to selectivity, linearity, detection and quantitation limits, accuracy, precision. The good analytical performance verified for this method indicates that it is suitable the monitoring of nitrite and nitrate in dairy drinks based on Zonar Milk Serum.

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“…Today, a wide variety of food analysis methods are based on modern separation techniques. There are several reports on the nitrate 14,15 and thiocyanate 16,17 contents of milk worldwide by ion chromatography (IC).…”

Section: Introductionmentioning

confidence: 99%

A sample stacking-capillary electrophoresis method for simultaneous determination of nitrate and thiocyanate ions of ultra-heat-treated milk samples

Tezcan¹

2018

Turk J Chem

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Ultra-heat-treated (UHT) cow milk is mostly consumed in Turkey. Heating milk can cause some chemical and biochemical changes that affect different components of milk. However, studies on the constituents of UHT milk are scare. In the present study, nitrate and thiocyanate contents of commercial UHT whole cow milk were determined simultaneously by sample stacking-capillary electrophoresis. The best separation of nitrate and thiocyanate ions was performed by reducing the electroosmotic flow at pH 4.0 using 30 mM formic acid and 30 mM sodium sulfate containing separation electrolyte. Analysis was completed in less than 3 min. The limits of detection obtained at a signal-to-noise ratio of 3 for nitrate and thiocyanate were 0.03 mg/L (0.48 µ mol/L) and 0.06 mg/L (1.03 µ mol/L), respectively. The nitrate concentration in commercial UHT milk samples ranged between 0.20 and 1.50 mg/L (3.23-24.20 µ mol/L) and the thiocyanate ion concentration ranged between 0.41 and 0.67 mg/L (7.06-11.55 µ mol/L).

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Simultaneous Determination of Nitrite and Nitrate in Milk Samples by Ion Chromatography Method and Estimation of Dietary Intake

Cited by 35 publications

References 25 publications

A Review of Nitrate and Nitrite Toxicity in Foods

A Review of Nitrate and Nitrite Toxicity in Foods

"Determination of nitrate and nitrite content in Zonar milk serum and derived dairy drinks using ion-pair reversed-phase high performance liquid chromatography "

A sample stacking-capillary electrophoresis method for simultaneous determination of nitrate and thiocyanate ions of ultra-heat-treated milk samples

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