Simultaneous Determination of Nitrate and Nitrite in Toothpastes by High-Performance Liquid Chromatography

Lulla, H.; Chen, S.S.; Sena, F.J.

doi:10.1002/jps.2600730740

Cited by 4 publications

(2 citation statements)

References 25 publications

(1 reference statement)

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“…Nitrite and nitrate were analyzed using HPLC by following the protocol of Lulla et al 30 Compounds were separated on an RP-C18 column (25 cm × 4 mm i.d. and 5 μm particle diameter; Phenomenex) with a mixed solution of (0.2%) sodium acetate and (2.5%) glacial acetic acid at a flow rate of 1.0 mL/min at 313 nm.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant compounds and activities of the stem, flower, and leaf extracts of the anti-smoking Thai medicinal plant: <em>Vernonia cinerea</em> Less

Ketsuwan¹,

Leelarungrayub²,

Kothan³

et al. 2017

DDDT

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Vernonia cinerea (VC) Less has been proposed as a medicinal plant with interesting activities, such as an aid for smoking cessation worldwide. Despite its previous clinical success in smoking cessation by exhibiting reduced oxidative stress, it has not been approved. The aim of this study was to investigate various antioxidant activity and active compounds that have not been approved, including the protective activity in human red blood cells (RBCs), from the stem, flower, and leaf extracts of VC Less in vitro. These extracts were tested for their antioxidant activity in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and analyzed by high-performance liquid chromatography (HPLC) for their active compounds: total tannin, five catechin (C) compounds (epicatechin gallate [ECG], C, epicatechin [EC], epigallocatechin gallate [EGCG], and (−)-epigallocatechin [EGC]), flavonoid, nitrite, nitrate, caffeine, and nicotine. Moreover, antioxidant activities of the extracts were evaluated in 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-treated RBCs. The results showed that the flower and leaf of VC Less had higher activity than the stem in scavenging DPPH radicals. The tannin content in the flower and leaf was higher than that in the stem. The leaf had the highest content of the five catechins (C, EC, EGCG, ECG, and EGC), the same as in the flavonoid, when compared to the stem and flower. Furthermore, the leaf extract had higher nitrate and nitrite than the stem. Nicotine content was found to be higher in the leaf when compared to the flower. In addition, the leaf showed protective activity in glutathione (GSH), malondialdehyde (MDA), and protein carbonyl, with a dose response in AAPH-oxidized RBCs, the same as in standard EGCG. Thus, this study concluded that radical scavenging and antioxidant compounds such as catechins, flavonoid, nitrate and nitrite, and nicotine are present in different VC Less parts and are included in the AAPH-oxidized RBC model.

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Section: Methodsmentioning

confidence: 99%

Antioxidant compounds and activities of the stem, flower, and leaf extracts of the anti-smoking Thai medicinal plant: <em>Vernonia cinerea</em> Less

Ketsuwan¹,

Leelarungrayub²,

Kothan³

et al. 2017

DDDT

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“…Linearity of method was 0.33-1.63 mgml − 1 (n = 5; r 2 = 0.75; r = 0.86, p = 0.01) with 1.45(± 0.14)-min retention times in a run of 15 min. Detection limit obtained at the intersection of the y-axis was 0.15 (54). NO 3 linear response was analysed in the interval of 0.7394 a 364.71 µgml − 1 (n = 5; r 2 = 0.99; r = 0.99, p = 0.0001) and…”

Section: Processing Of Samples and Analyticalmentioning

confidence: 99%

Tolerance to Nitrogen ions enrichment in a Gulf of Mexico's freshwater submerged grass

Ruiz-Carrera

Sánchez

Leal

2020

Preprint

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BackgroundTolerance to the enrichment of ionic nitrogen (N) with ammonium (NH 4 ) and nitrate (NO 3 ) needs to be distinguished in a submerged grass ecotype-species of freshwater wetlands. Concentrations of total Nitrogen (TN: 500 to 2000 µgL -1 ) and three N sources (NS: NH 4 , NO 3 , 1:1 NH 4 :NO 3 ratio) in an overlaying aqueous phase, using local tap water as control (174 µgL -1 TN), were evaluated in rooted autotrophic juvenile Vallisneria americana established in vitro in two-phase culture medium. The treatments ranged from 7 to 111 µM TN. Phenotypes changes, pH and ionic strength in the aqueous phase were registered at 15 days. In addition, biomass of lyophilized leaf and root as well as N contents (gas and high-performance liquid chromatography) were analysed to estimate accumulations of N-NH 4 , N-NO 3 and N-NO 2 .ResultsAll individuals showed phenotypic similarities. Accumulation of N-NH 4 was discarded, but the accumulations of N-NO 3 and N-NO 2 in both tissues were significantly different (p<0.001 and 0.0001), revealing so much that the maximum cumulative responses coincided with a deficit of N as both showed a linear decrease in their cumulative capacity linked to an increase in micromolar TN. ConclusionsThe results provide direct evidence of the tolerance to N enrichment with three different N sources in the focal ecotype at the seedling stage, based mainly on its cumulative capacity of N-NO 3 and N-NO 2 under eutrophic conditions. The findings imply that NO 2 is a relevant ion in the tolerance to N enrichment in overlaying water with NH 4 and NO 3 ions. Future studies of N cumulative effect with an in vitro approximation will be to support stress predictions by N enrichment. Keywords : Hydrocharitaceae. Eutrophization. Stress in vitro. Ammonium. Nitrate. Nitrogen endogenous

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Analytical Methods for Actives used in General and Specific Skin-Care, Personal Hygiene and other Toiletry Products (Excluding those Mentioned in Previous Chapters)

Balaguer

Chisvert

Sisternes

et al. 2007

Analysis of Cosmetic Products

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Simultaneous Determination of Nitrate and Nitrite in Toothpastes by High-Performance Liquid Chromatography

Cited by 4 publications

References 25 publications

Antioxidant compounds and activities of the stem, flower, and leaf extracts of the anti-smoking Thai medicinal plant: <em>Vernonia cinerea</em> Less

Antioxidant compounds and activities of the stem, flower, and leaf extracts of the anti-smoking Thai medicinal plant: <em>Vernonia cinerea</em> Less

Tolerance to Nitrogen ions enrichment in a Gulf of Mexico's freshwater submerged grass

Analytical Methods for Actives used in General and Specific Skin-Care, Personal Hygiene and other Toiletry Products (Excluding those Mentioned in Previous Chapters)

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