Simultaneous Determination of Nine Sulphonamides by LC-MS for Routine Control of Raw Honey Samples

Guillén, Isidro; Guardiola, Lucía; Almela, L.; Núñez‐Delicado, Estrella; Gabaldón, José Antonio

doi:10.1007/s12161-016-0698-9

Cited by 16 publications

(4 citation statements)

References 31 publications

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“…Liquid chromatography-Mass spectrometry tandem (LC-MS/MS) is the most applied technique for this purpose due to its high sensitivity and selectivity. However, it is an expensive analytical method, with a long and multistage sample preparation that makes the analysis time-consuming, labor-intensive, and unsuitable for routinely monitoring sulphonamide levels (Guillén, Guardiola, Almela, Núñez-Delicado, & Gabaldón, 2017;Juan-Borrás, Periche, Domenech, & Escriche, 2015;Louppis, Kontominas, & Papastephanou, 2017).…”

Section: Introductionmentioning

confidence: 99%

High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey

et al. 2020

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In this study, a piezoelectric immunosensor based on High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) technology was developed for detection of sulfathiazole in honey. The biorecognition was based on a competitive immunoassay in the conjugate-coated format, using monoclonal antibodies as specific immunoreagents.The quantification of sulfathiazole was performed by building the corresponding calibration standard curve in diluted honey (1/140). Due to the competitive nature of the immunoassay, the standard curve showed a sigmoidal pattern with limits of detection (LOD) and quantification (LOQ) of 0.10 µg/kg and 2 µg/kg honey, respectively. The 2 LOD reached by this immunosensor is 40-50 times lower than those reported by other techniques for antibiotic detection. Moreover, this method requires minimum honey pre-treatment, making it faster and simpler than other methods. This immunosensor meets the precision and accuracy requirements established by SANCO guidelines, when sulfathiazole concentration in honey is not lower than 10 µg/kg. These findings could be the basis for reaching enough reliability for lower concentrations. Therefore, HFF-QCMD immunosensors can be considered a feasible alternative to current techniques for rapid and highly sensitive determination of sulfathiazole in honey with minimum sample preparation.

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Section: Introductionmentioning

confidence: 99%

High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey

et al. 2020

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“…e result has been sulfonamide residues in foods of animal origin (milk, meat, eggs, sh, or honey). is is a potential hazard for consumers in terms of the development of resistant microorganisms, allergic reactions, or toxic e ects [1,2]. One speci c application of sulfonamides is their use on bees.…”

Section: Introductionmentioning

confidence: 99%

“…e predominant separation method for determination of sulfonamides in samples is liquid chromatography in combination with tandem mass spectrometry (LC-MS/MS). Fluorescence detection following pre-or postcolumn derivatization with fluorescamine is also used [1,8,[14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Sulfonamide Residues: Honey Quality in the Czech Market

Dluhošová

Borkovcová

Kaniová

et al. 2018

Journal of Food Quality

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In this study, we determined four sulfonamide compounds (sulfadiazine, sulfadoxine, sulfamethoxazole, and sulfathiazole) in honey marketed in the Czech Republic (Brno). The content of these compounds was monitored in 50 honeys with different botanical and geographical origin. Preanalytical treatment included acidic hydrolysis with 10% trichloroacetic acid (TCA) and double extraction with acetonitrile and dichloromethane. Chromatographic analysis was performed by ultraperformance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) using a triple quadrupole (QqQ) in positive ionization mode (ESI+). For separation, the mobile phase was a 0.05% aqueous solution of formic acid (A) and 0.05% formic acid in methanol (B) with a gradient elution of 0–3 minutes: 10% B; 3–3.6 min: 90% B; and 3.6–5 min: 10% B, flow rate 0.30 ml/min. Three positive samples (6%) were found containing sulfadiazine (232.88 and 618.87 μg/kg) and sulfamethoxazole (62.64 μg/kg). According to the country of origin, two of these honeys were from European Union (EU) countries, and one was labeled as a blend of honey from EU and non-EU countries.

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“…Liquid chromatography-Mass spectrometry tandem (LC-MS/MS) is the most applied technique for this purpose due to its high sensitivity and selectivity. However, it is an expensive analytical method, with a long and multistage sample preparation that makes the analysis timeconsuming, labor-intensive, and unsuitable for routinely monitoring sulphonamide levels (Juan-Borrás et al, 2015;Guillén et al, 2017;Louppis et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Aplicación y validación de la tecnología de inmunosensores piezoeléctricos de alta frecuencia (HFF-QCM) para la detección de pesticidas y antibióticos en miel

Chiner¹

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Les troballes obtinguts en aquesta tesi són prometedors i estableixen un punt de partida per a futurs desenvolupaments encaminats a aconseguir la detecció simultània de diversos analits en un únic anàlisi i mostra. Açò podria aconseguir-se amb una matriu de sensors HFF-QCM.En definitiva, la investigació realitzada posa de manifest que els inmunosensors basats en la tecnologia HFF-QCM són una alternativa seriosa a les tècniques actuals, per a la quantificació in situ de pesticides i antibiòtics en la indústria d'envasament de la mel.

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Simultaneous Determination of Nine Sulphonamides by LC-MS for Routine Control of Raw Honey Samples

Cited by 16 publications

References 31 publications

High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey

High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey

Sulfonamide Residues: Honey Quality in the Czech Market

Aplicación y validación de la tecnología de inmunosensores piezoeléctricos de alta frecuencia (HFF-QCM) para la detección de pesticidas y antibióticos en miel

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