Simultaneous determination of nine saponins from Panax notoginseng using HPLC and pressurized liquid extraction

Wan, Jian‐Bo; Lai, Chang-Jiang-Sheng; Li, Shao‐Ping; Lee, M.Y.; Kong, Ling–Yi; Wang, Y.T.

doi:10.1016/j.jpba.2005.10.023

Cited by 75 publications

(41 citation statements)

References 27 publications

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“…Qualitative and quantitative determination of herbal medicine is an important issue for the safety and efficacy of herbal medicine [3,29]. Some analytical studies have been conducted for the determination of the saponins of notoginseng [8,26]. However, almost all studies have focused on the root extract.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we analyzed the saponin composition of NGF. Compared with the root extract [8,26], the chemical composition of notoginseng flower has special aspects. In notoginseng root, the main constituents are notoginsenoside R1, ginsenoside Rb1, Re and Rg1.…”

Section: Discussionmentioning

confidence: 99%

“…The chemical composition of notoginseng could be different based on its origin. We selected P. notoginseng cultivated in Wenshan for our study since the quality of notoginseng from Wenshan has been controlled under the Good Agricultural Practice criteria [5], and has been frequently used in many previous studies [8,26]. This notoginseng was obtained from Yunnan Chinese Herbal Medicine Company and the root and flower were collected in 2004 and was authenticated by the Department of Pharmacognosy, China Pharmaceutical University, Nanjing.…”

Section: Plant Materialsmentioning

confidence: 99%

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Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells

Wang

Luο

Zhang

et al. 2006

Cancer Chemother Pharmacol

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Purpose-Panax notoginseng is a commonly used Chinese herb. Although a few studies have found that notoginseng shows anti-tumor effects, the effect of this herb on colorectal cancer cells has not been investigated. 5-Fluorouracil (5-FU) is a chemotherapeutic agent for the treatment of colorectal cancer that interferes with the growth of cancer cells. However, this compound has serious side effects at high doses. In this study, using HCT-116 human colorectal cancer cell line, we investigated the possible synergistic anti-cancer effects between notoginseng flower extract (NGF) and 5-FU on colon cancer cells.Methods-The anti-proliferation activity of these modes of treatment was evaluated by MTS cell proliferation assay. Apoptotic effects were analyzed by using Hoechst 33258 staining and Annexin-V/PI staining assays. The anti-proliferation effects of four major single compounds from NGF, ginsenosides Rb1, Rb3, Rc and Rg3 were also analyzed.Results-Both 5-FU and NGF inhibited proliferation of HCT-116 cells. With increasing doses of 5-FU, the anti-proliferation effect was slowly increased. The combined usage of 5-FU 5 μM and NGF 0.25 mg/ml, significantly increased the anti-proliferation effect (59.4 ± 3.3%) compared with using the two medicines separately (5-FU 5 μM, 31.1 ± 0.4%; NGF 0.25 mg/ml, 25.3 ± 3.6%). Apoptotic analysis showed that at this concentration, 5-FU did not exert an apoptotic effect, while apoptotic cells induced by NGF were observed, suggesting that the anti-proliferation target(s) of NGF may be different from that of 5-FU, which is known to inhibit thymidilate synthase.Conclusions-This study demonstrates that NGF can enhance the anti-proliferation effect of 5-FU on HCT-116 human colorectal cancer cells and may decrease the dosage of 5-FU needed for colorectal cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

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Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells

Wang

Luο

Zhang

et al. 2006

Cancer Chemother Pharmacol

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“…However, those studies on its anticancer properties focused largely on the crude extracts of P. notoginseng, 5,[7][8][9] and the saponins identified in the different crude extracts were fluctuant because of low purity. 5,[10][11][12] For this reason, much more research effort is needed to elucidate the antitumor profiles in a variety of cancer cell types and how P. notoginseng saponin extract (PNSE) influences cancer development and recurrence. It is also very necessary to evaluate the potential anticancer effect of a more purified saponin extract in order to understand the nature of PNSE contribution and its real application as a potential cancer chemopreventive agent.…”

Section: Introduction Cmentioning

confidence: 99%

Antioxidant, Antiproliferative, and Pro-Apoptotic Activities of a Saponin Extract Derived from the Roots of Panax notoginseng (Burk.) F.H. Chen

Zhao

Guo

et al. 2012

Journal of Medicinal Food

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Dietary and medicinal uses of Panax notoginseng have been associated with reduced risk of cancer. This study was designed to investigate the profiles of P. notoginseng saponin extract (PNSE), the major bioactive ingredients in P. notoginseng (Burk.) F.H. Chen, by high-performance liquid chromatography, and, for the first time, the anticancer effect of PNSE in the human colon cancer cell line LoVo was further evaluated. The major saponins present in PNSE were ginsenosides Rg1 (31.1%) and Rb1 (34.4%), and the total content of the eight saponins identified (notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, and Rd, and isomeric ginsenosides Rb2 and Rb3) was 81.7%, indicating that it was a highly purified standardized saponin extract. Furthermore, PNSE was found to have a markedly cytotoxic effect and antiproliferative activity against the LoVo cell line in a dose-and time-dependent manner. Flow cytometry analysis demonstrated that PNSE caused cell cycle arrest at S phase. Moreover, PNSE was found to possess antioxidative capacities in the 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay and hydroxyl radical scavenging assay in vitro. Taken together, the present results suggest that naturally occurring PNSE may provide significant natural defense against human colon cancer.KEY WORDS: antioxidant activity antitumor activity ginsenoside high-performance liquid chromatography notoginsenoside Panax notoginseng

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“…In the extensive phytochemical and pharmacological studies on P. notoginseng, 56 dammarane-type saponins, the main bioactive components, have been isolated and characterized from the roots, leaves and flower buds (Wang et al, 2006a). The protopanaxadiol-type saponins (ginsenosides Rb 1 , Rb 2 , Rb 3 , Rc, F 2 and Rd, and notoginsenosides Fe and Fc) and protopanaxatriol-type saponins (ginsenosides Re, Rg 1 and Rh 1 , and notoginsenosides R 1 , R 2 and R 6 ) are considered to be the main active saponins in the flower bud of P. notoginseng, and the triterpene saponin fraction from flower buds has shown a protective effect on liver injury induced by D-galactosamine and lipopolysaccharide (Yoshikawa et al, 2003) and an enhanced anti-proliferation effect on 5-fluorouracil in human colorectal cancer cells (Wang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of saponins in flower buds of Panax notoginseng using high performance liquid chromatography

Gao

Dan

Zhao

et al. 2007

Biomedical Chromatography

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The flower buds of Panax notoginseng have been commonly used for the treatment of hypertension, vertigo, tinnitus and acute faucitis in China. The amount of total saponins in the flower buds is higher than in any other parts of P. notoginseng. However, the compositions of flower buds have not been quantified clearly until now. A sensitive and efficient high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for the first time to simultaneously quantify eight active saponins in the flower buds of P. notoginseng, including notoginsenoside R(1) and ginsenosides Rg(1), Re, Rb(1), Rb(2), Rb(3), Rd and F(2). The analysis was performed on a reversed-phase C(18) column with gradient elution of acetonitrile and 0.01% aqueous formic acid. The proposed method provided good linearity, reproducibility and sensitivity for the simultaneous quantification of the investigated saponins with overall intra- and inter-day precision and accuracy of better than 4.1% (RSD) and higher than 95% (accuracy), respectively. The recoveries for all the saponins determined were in the range 94.7-104.8% with RSD better than 3.1%. Using the optimized method, we were able to analyze samples from different villages of Wenshan Prefecture, China, which is helpful for quality control of flower buds of P. notoginseng.

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Simultaneous determination of nine saponins from Panax notoginseng using HPLC and pressurized liquid extraction

Cited by 75 publications

References 27 publications

Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells

Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells

Antioxidant, Antiproliferative, and Pro-Apoptotic Activities of a Saponin Extract Derived from the Roots of Panax notoginseng (Burk.) F.H. Chen

Simultaneous determination of saponins in flower buds of Panax notoginseng using high performance liquid chromatography

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