Simultaneous determination of nine intense sweeteners in foodstuffs by high performance liquid chromatography and evaporative light scattering detection—Development and single-laboratory validation

Wasik, Andrzej; McCourt, Josephine; Buchgraber, Manuela

doi:10.1016/j.chroma.2007.04.068

Cited by 130 publications

(66 citation statements)

References 29 publications

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“…Precision was tested by fortification of sweeteners in three matrices at fi three different concentration levels on three diff ff ff erent days. Intermediate ff ff precision was < 8% for all sweeteners with the exception of ASP in canned fruits, due to its degradation as a result of improper storage conditions [2]. This method is suitable for rapid screening of samples for sweeteners but Th may lack selectivity for target analytes, especially among interferences in the matrix.…”

Section: Hplc-uv-vis/dad/elsd Detectionmentioning

confidence: 99%

“…Determination of nine sweeteners by HPLC with evaporative light scattering detection (ELSD) has been published [2]. The method involves SPE Th cleanup of samples prior to HPLC-ELSD.…”

Section: Hplc-uv-vis/dad/elsd Detectionmentioning

confidence: 99%

“…Th ey are Th an important class of food additives which are added to foods to cause *Corresponding author: romina.shah@fda.hhs.gov a technical effect such as sweetening [2]. Sweeteners are grouped into ff ff two main categories, bulk and intense sweeteners.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent Analytical Methods for the Analysis of Sweeteners in Food: A Regulatory Perspective

Shah

Jager

2016

Food Safety

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Section: Hplc-uv-vis/dad/elsd Detectionmentioning

confidence: 99%

“…Determination of nine sweeteners by HPLC with evaporative light scattering detection (ELSD) has been published [2]. The method involves SPE Th cleanup of samples prior to HPLC-ELSD.…”

Section: Hplc-uv-vis/dad/elsd Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Recent Analytical Methods for the Analysis of Sweeteners in Food: A Regulatory Perspective

Shah

Jager

2016

Food Safety

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“…High-performance liquid chromatography (HPLC) has been the most popular choice for the determination of additives (Wasik et al 2007;Zygler et al 2011;; however, other analytical methods have also been applied in their analysis (Bergamo et al 2011;Chen and Wang 2001;Zhu et al 2005) but the method using HPLC-Corona charged aerosol detector (CAD) has not been reported up-to-date. The Corona CAD is a new type of detector introduced for HPLC applications and has recently become widely used (Błażewicz et al 2010;Eom et al 2010;Kou et al 2009;Lísa et al 2007;Nováková et al 2009;Schönherr et al 2009;Takahashi et al 2008;Vervoort et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Caffeine and Aspartame in Diet Supplements and Non-Alcoholic Beverages Using Liquid-Chromatography Coupled to Corona CAD and UV-DAD Detectors

Grembecka

Szefer

2011

Food Anal. Methods

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A method for the simultaneous determination of caffeine and aspartame in various diet supplements and nonalcoholic beverages is presented. The analytes were analysed by high-performance liquid chromatography coupled to a charged aerosol detector (Corona CAD) and ultravioletdiode array detector (UV-DAD) simultaneously connected in series. The method was validated using a Thermo Hypersil Gold-C18 column packed with 5 μm shell particles (150×4.6 mm) and acetonitrile-water (15/85% v/v) mobile phase at a flow rate of 1.00 mL/min. The elaborated method was validated for linearity, precision and accuracy. The rapid HPLC-CAD-UV-DAD technique is suitable for quantifying caffeine and aspartame in the range of 0.25-75 μg/mL in diet supplements and non-alcoholic beverages. The limit of detection for caffeine and aspartame was 62 and 43 ng/mL for Corona CAD and 31 and 30 ng/mL for UV-DAD detector, respectively. Each analyte calibration curve had a correlation coefficient of at least 0.999 and was linear in the defined range. The accuracies of CAD and UV-DAD detection were all acceptable, with the mean value of 100% for aspartame and 98.3% for caffeine. Precision values ranged from 0.09% to 1.12%. The work has demonstrated that charged aerosol detector can be successfully used in a rapid screening technique for biologically active substances in non-alcoholic beverages and diet supplements.

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“…Detection by indirect spectrophotometry (Herrmann et al 1983), absorption in the visible range with the addition of dyes (Choi et al 2000), absorption in the ultraviolet range (UV) and fluorescence prior to sample derivatisation (Hauck & Köbler 1990;Choi et al 2000), MS (Zygler et al 2011), a light-scattering evaporative detector (Wasik et al 2007), and capillary zone electrophoresis (Vistuba et al 2015) have been used.…”

Section: Introductionmentioning

confidence: 99%

Validation of an HPLC-DAD/UV method for the quantification of cyclamate in tabletop sweeteners: risk of exceeding the acceptable daily intake

Sargaço

Serra

Vasco

2017

Food Additives & Contaminants: Part A

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Cyclamate is widely used as intense sweetener in the European Union. The absence of a maximum limit for the use of cyclamate in tabletop sweeteners and the growing demand for this type of product highlights the importance of developing robust analytical methods for the determination of its content to understand if the consumption of tabletop sweeteners can have a negative impact on human health. The present work aimed at the optimisation and validation of an high-performance liquid chromatography (HPLC) analytical method for cyclamate determination in tabletop sweeteners based on the procedure of European Standard EN 12857. The validated method was then applied to the determination of this sweetener in different types of tabletop sweeteners (liquid, powder and tablets). Both standards and samples solutions were submitted to a derivatisation procedure which converted cyclamate to N,N-dichlorocyclohexylamine. The derivatised product was separated and quantified using a reversed-phase column, a mobile phase composed of water (20%) and methanol (80%), isocratic flow of 1 ml min -1 , and detection by ultraviolet spectrophotometry at a wavelength of 314 nm. The analytical method was internally validated according to the following validation parameters: working range, linearity, limits of detection and quantification, sensitivity, precision (repeatability and intermediate precision), and uncertainty. This method proved to be specific and selective for the determination of this sweetener, showing repeatability, RSD r ≤ 3%, intermediate precision, RSD R ≤ 3.3%, and recovery rates from 92% to 108% for the different tabletop sweeteners. The method uncertainty was 9.4%. The concentration of cyclamate in the samples varied significantly, from 2.9% to 73.9%, which demonstrated that a possible excessive consumption of one of the analysed sweeteners can lead to exceeding the acceptable daily intake for cyclamate. ARTICLE HISTORY

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Simultaneous determination of nine intense sweeteners in foodstuffs by high performance liquid chromatography and evaporative light scattering detection—Development and single-laboratory validation

Cited by 130 publications

References 29 publications

Recent Analytical Methods for the Analysis of Sweeteners in Food: A Regulatory Perspective

Recent Analytical Methods for the Analysis of Sweeteners in Food: A Regulatory Perspective

Simultaneous Determination of Caffeine and Aspartame in Diet Supplements and Non-Alcoholic Beverages Using Liquid-Chromatography Coupled to Corona CAD and UV-DAD Detectors

Validation of an HPLC-DAD/UV method for the quantification of cyclamate in tabletop sweeteners: risk of exceeding the acceptable daily intake

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