Simultaneous determination of multicomponent of acetylkitasamycin and kitasamycin by LC–MS/MS in swine plasma and its application in a pharmacokinetic study

Abstract

A simple and reliable LC-MS/MS method was established for simultaneous determination of 12 components from acetylkitasamycin and kitasamycin in swine plasma. The analytes were separated on a Shim-pack VP-ODS column with a 25 min gradient elution using 5 mmol/L ammonium acetate and acetonitrile as the mobile phase at a flow rate of 0.2 mL/min. Identification and quantification were accomplished by electrospray ionization) in positive mode using multiple reaction monitoring. The limits of quantitation of acetylkitasamycin A A , A and kitasamycin A , A were 3 μg/L, and that of the other eight components was 5 μg/L. The mean recoveries of kitasamycin and acetylkitasamycin ranged from 85.3 to 103.5%. The developed method was successfully applied to a pharmacokinetic study in swine after intravenous (i.v.) and oral (p.o.) administration of acetylkitasamycin. The result showed that the plasma concentrations of acetylkitsamycin components were much higher than that of kitasamycin in swine after i.v. and p.o., in which acetylkitsamycin A A was the highest component at each time point.

PK-PD Modeling and Optimal Dosing Regimen of Acetylkitasamycin against Streptococcus suis in Piglets

“…Quantitation of acetylkitasamycin and kitasamycin in every component of piglet plasma and PELF was conducted using the sensitive and selective high-performance liquid chromatographic mass spectrometry (LC-MS/MS) method [ 34 ]. The plasma specimens (0.25 mL) and BAL specimens (0.25 mL) were thawed and added to a 10 mL centrifuge tube with 0.75 mL acetonitrile, and vortexed for 3 min (12,000 r/10 min); 1 mL ethylacetate was added to the supernatant and vortexed for 3 min (12,000 r/10 min), and then the supernatant was taken out.…”