Simultaneous determination of modafinil and its acid metabolite by high-performance liquid chromatography in human plasma

Detection of modafinil in human urine by gas chromatography–mass spectrometry

“…Our GC-MS results differed from previous studies using HPLC in that we identified only one chromatographic peak rather than two (modafinil acid and modafinil sulfone) [2,13,15]. The reasons for this discrepancy may be attributed a number reasons and merits further investigation.…”

“…Later in 2004, modafinil was added to the stimulant-drug list prohibited by the World Anti-Doping Agency (WADA) [9]. Previous detection of this prohibited drug was performed analytically by HPLC [1,2,[10][11][12][13]. HPLC is commonly used in sport-related testing as an initial screen for certain drugs (e.g.…”

Modafinil effects in multiple sclerosis patients with fatigue

“…Patients were studied prior to their drug ingestion (T1), 3 hours after the first intake (T2) which corresponds to the maximum MOD plasma level [17], and after 8 weeks of drug application (T3).…”

Determination of modafinil in plasma and urine by reversed phase high-performance liquid-chromatography

“…The analytical method reported here also differs from the previously published methods in that we used ethyl acetate or ethyl acetate-acetic acid (100:1, v/v) as the extraction solvent whereas the previous procedures used hexane-dichloromethane-acetic acid, 55:45:2, v/v/v [4] or a solid phase absorbent [6,8]. We also used methanol-water-acetic acid (500:500:1, v/v/v) as the mobile phase whereas the previous procedures used acetonitrile-0.02 M potassium phosphate buffer (30:70, v/v; pH 2.5 for plasma samples and pH 4.0 urine samples) [5][6][7], a combination of acetonitrile and orthophosphoric acid [8] or acetonitrile and acetic acid [9]. The analytical method reported here, eliminates the possible toxic hazards associated with the use of dichloromethane and acetonitrile and the effects of acidic phosphate salt buffers on the chromatographic columns and pumps.…”

“…Several HPLC methods have been developed for the analysis of modafinil and its metabolites in plasma and urine [5][6][7][8][9] pharmacokinetic studies of modafinil and its enantiomers [5][6][7]. In those procedures, samples were extracted with hexane-dichloromethane-acetic acid (55:45:2, v/v/v) or by solid phase extraction and analyzed on either phenyl columns or ␤-cyclodextrin columns.…”