Simultaneous determination of mexiletine and four hydroxylated metabolites in human serum by high-performance liquid chromatography and its application to pharmacokinetic studies

Pharmacologically Active Drug Metabolites: Impact on Drug Discovery and Pharmacotherapy

“…Mexiletine is a sodium channel blocker used as an antiarrythmic agent. In humans it is converted to several hydroxyl metabolites (Paczkowski et al, 1992), two of which have been shown to have in vitro activity (p-hydroxymexiletine and hydroxymethylmexiletine) (Fig. 80) (Catalano et al, 2004;DeBellis et al, 2006).…”

Pharmacokinetic study of three cardiovascular drugs by high-performance liquid chromatography using pre-column derivatization with 9,10-anthraquinone-2-sulfonyl chloride

“…After a pre-column derivatization, both the detection limit and the chromatographic behavior of each drug were improved significantly. Compared to the other methods [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] reported previously, this method was accurate, sensitive, and simple. It has been successfully applied to the pharmacokinetic study of three drugs in rats, and the pharmacokinetic characteristics were revealed.…”

“…Various methods have been reported for analyzing MXL, MTX, and MTR [4][5][6][7][8][9][10][11][12][13][14][15][16]. The previously published methods using gas chromatography (GC) [4,5], high-performance liquid chromatography (HPLC) [6][7][8], and high-performance capillary electrophoresis (HPCE) [9], however, were not sensitive enough, because their lower limit of quantification without removal of light or a post-derivatization treatment to inactivate excessive reagents [22][23][24].…”

“…The previously published methods using gas chromatography (GC) [4,5], high-performance liquid chromatography (HPLC) [6][7][8], and high-performance capillary electrophoresis (HPCE) [9], however, were not sensitive enough, because their lower limit of quantification without removal of light or a post-derivatization treatment to inactivate excessive reagents [22][23][24]. A considerable improvement was made of the polarity and UV absorption of the ASC derivatives [22][23][24], which are beneficial to the analysis of the trace drugs in biological fluids.…”

Column-Switching HPLC Determination of Mexiletine Using Tris(bipyridine)ruthenium(III) Electrogenerated Chemiluminescence and Precolumn Derivatization with Divinylsulfone

“…Several analytical HPLC methods utilizing UV [16][17][18][19][20] and fluorometric [21][22][23][24] A sensitive HPLC method combined with a column-switching system and tris(bipyridine)ruthenium(III) electrogenerated chemiluminescence (ECL) detection has been developed for the quantitative determination of mexiletine (MEX). MEX was derivatized by divinylsulfone (DVS) and then measured.…”