Simultaneous determination of methylphenidate and ritalinic acid in hair using LC–MS/MS

Jang, Moonhee; Kim, Jihyun; Shin, Injae; Kang, Soo Cheol; Choi, Hyeyoung; Yang, Wenjun

doi:10.1016/j.forsciint.2018.11.021

Cited by 7 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both Jang et al 43 and Marchei et al 42 presented successful method validations for the detection of methylphenidate in hair using LC–MS/MS. Jang et al 43 applied the method to hair samples from 10 drug users with illegal methylphenidate use, whereas Marchei et al 42 investigated the hair samples of 11 children under known dosage regimen for methylphenidate for at least 6 months. The hair samples were analyzed in 3‐cm segments in both studies.…”

Section: Methods Development and Validation Publicationsmentioning

confidence: 99%

Hair analysis of antidepressants and antipsychotics—Overview of quantitative data

Methling

Krumbiegel

Härtwig

2020

Drug Testing and Analysis

View full text Add to dashboard Cite

Antidepressant and antipsychotic drugs are regularly encountered in different aspects of forensic toxicology, and some cases require the examination of hair samples. In this study, common antidepressant and antipsychotic drugs regarding hair concentrations over the past decades were reviewed. Although numerous publications around method validations, case reports, or controlled dose studies were found, apparently there is a lack of comprehensive data for many substances. Information on the hair length and dosage across the publications varied largely, and case numbers were generally low except for several retrospective controlled dose studies. Many substances were described only in method validations or case reports, and data were obtained from small case numbers. On the contrary, clozapine, haloperidol, amitriptyline, nortriptyline, risperidone and its metabolite, methylphenidate, citalopram, chlorpromazine, chlorprothixene, and quetiapine had a well‐founded database as these substances were investigated in controlled dose studies with higher case numbers. Given the advancements made in analytical techniques over the past years, gas chromatography–mass spectrometry and liquid chromatography with tandem mass spectrometry techniques were the methods of choice and allowed the detection of chemical compounds at low concentrations. The controversy around a potential use of hair analysis to estimate the dosage remains as dose‐concentration studies provided divergent results. A harmonization on the investigated hair length as well as on the extraction protocol would be of favor to achieve better comparability. Although hair analysis research focused mainly on drug abuse, availability of more data on antidepressants and antipsychotics would help to gain better knowledge and assist other forensic investigators.

show abstract

Section: Methods Development and Validation Publicationsmentioning

confidence: 99%

Hair analysis of antidepressants and antipsychotics—Overview of quantitative data

Methling

Krumbiegel

Härtwig

2020

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…Fig. 1 Calibration parameters according to GTFCH guideline for the validation of analytical methods [28]: weighting factor, (R) correlation factor, (LOD) limit of detection, (LOQ) limit of quantification, and linear range (ng/mg) expressed as yellow bar A review of published hair concentrations for the different analytes in this study was made [13][14][15][16][17][18][19][20][21][22][23][24][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48]. No published data were found for comparison for opipramol, trimipramine, fluvoxamine, O-desmethylvenlafaxine, benperidol, pipamperone, fluphenazine, and N-desmethylclozapine.…”

Section: Concentrations Detected In Hair: Meaning and Considerationsmentioning

confidence: 99%

Concentrations of Antidepressants, Antipsychotics, and Benzodiazepines in Hair Samples from Postmortem Cases

Methling

Krumbiegel

Härtwig

et al. 2020

SN Compr. Clin. Med.

View full text Add to dashboard Cite

Certain postmortem case constellations require intensive investigation of the pattern of drug use over a long period before death. Hair analysis of illicit drugs has been investigated intensively over past decades, but there is a lack of comprehensive data on hair concentrations for antidepressants, antipsychotics, and benzodiazepines. This study aimed to obtain data for these substances. A LC-MS/MS method was developed and validated for detection of 52 antidepressants, antipsychotics, benzodiazepines, and metabolites in hair. Hair samples from 442 postmortem cases at the Institute of Legal Medicine of the Charité-University Medicine Berlin were analyzed. Postmortem hair concentrations of 49 analytes were obtained in 420 of the cases. Hair sample segmentation was possible in 258 cases, and the segments were compared to see if the concentrations decreased or increased. Descriptive statistical data are presented for the segmented and non-segmented cases combined (n = 420) and only the segmented cases (n = 258). An overview of published data for the target substances in hair is given. Metabolite/parent drug ratios were investigated for 10 metabolite/parent drug pairs. Cases were identified that had positive findings in hair, blood, urine, and organ tissue. The comprehensive data on postmortem hair concentrations for antidepressants, antipsychotics, and benzodiazepines may help other investigators in their casework. Postmortem hair analysis results provide valuable information on the drug intake history before death. Pattern changes can indicate if drug intake stopped or increased before death. Results should be interpreted carefully and preferably include segmental analysis and metabolite/parent drug ratios to exclude possible contamination.Key Points 1. A sensitive and robust LC-MS/MS method for the detection of antidepressants, antipsychotics, and benzodiazepines in hair was developed, validated, and applied to 420 cases. 2. Comprehensive data on postmortem hair concentrations for 41 substances and their respective metabolites are given. 3. Metabolite/parent drug ratios provide valuable information for interpretation of postmortem hair concentrations. 4. Segmental hair analysis in postmortem toxicology can provide useful information on changes in the pattern of drug use. 5. An overview of quantitative data published in the literature for antidepressants, antipsychotics, and benzodiazepines in hair revealed a lack of comprehensive data.

show abstract

“…The analyte and IS were detected using MRM mode with m/z 56.1, 84.2 and 234.1 for the analyte and 183.1 and 260.2 for the IS, respectively [39]. Other LC-MS/MS methods for quantification of MPH in human plasma [135][136][137], blood, oral fluids [137], hair [138], and urine samples [139,140] are reported in Table 2.…”

Section: Liquid Chromatography (Lc)mentioning

confidence: 99%

Review on analytical methods for quantification of ADHD drugs in human biological samples

Sundari¹,

Amuthalakshmi²,

Nalini³

2020

Reviews in Analytical Chemistry

View full text Add to dashboard Cite

Attention deficit hyperactivity disorder (ADHD) is a common neuro-developmental disorder. The symptoms of ADHD include difficulty in attention, memory and impulse control. Many pharmaceutical formulations (stimulants and non-stimulants) are available on the market to treat ADHD symptoms. The most commonly used drugs for treatment are amphetamine, methylphenidate, atomoxetine, bupropion, guanfacine and clonidine. In the field of pharmaceuticals, bioanalysis is an important tool used for the quantification of drugs and their metabolites present in biological samples using various analytical methods. Although a number of analytical methods were reported for the quantification of these drugs in biological samples of experimental animals, due to species differences, it is important to develop analytical methods to quantify these drugs in human biological samples to aid forensic and pharmacokinetic studies. In this review, we compile the bio-analytical methods such as spectrophotometry, spectrofluorimetry, mass spectrometry, electrophoresis, liquid chromatography and gas chromatography used for the quantification of ADHD drugs in human biological samples such as blood, plasma, serum, oral fluids, sweat, hair and urine based on earlier published articles from various journals.

show abstract

Simultaneous determination of methylphenidate and ritalinic acid in hair using LC–MS/MS

Cited by 7 publications

References 30 publications

Hair analysis of antidepressants and antipsychotics—Overview of quantitative data

Hair analysis of antidepressants and antipsychotics—Overview of quantitative data

Concentrations of Antidepressants, Antipsychotics, and Benzodiazepines in Hair Samples from Postmortem Cases

Review on analytical methods for quantification of ADHD drugs in human biological samples

Contact Info

Product

Resources

About