Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD

Viegas, Olga; Esteves, Cristina; Rocha, Janete; Melo, Armindo; Ferreira, Isabel M. P. L. V. O.

doi:10.1016/j.foodchem.2020.128091

Cited by 32 publications

(15 citation statements)

References 34 publications

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“…Based on the values of extraction recovery obtained (between 95–104%), comparable to the results obtained by Viegas [ 14 ], we can conclude that the proposed DLLME method is the most appropriate and can be successfully applied to extraction and concentration of melatonin in wine samples, before analysis by HPLC-FL.…”

Section: Resultssupporting

confidence: 85%

“…HPLC is the sole trustworthy technology since it has a large capacity for discrimination, permits unambiguous identification, and performs one of the most precise analyses. For the analysis of the wine samples, the following procedures were used: HPLC with diode array detection (HPLC-DAD) [ 12 ], HPLC-FL using the native fluorescence of melatonin [ 3 , 13 , 14 ], and HPLC tandem mass spectrometry (HPLC-MS/MS) [ 15 , 16 ]. Even though the MS detector is more sensitive than the FL detector, HPLC-MS/MS is too complex and costly, making HPLC-FL more cost-effective and suited for assaying melatonin in wines, particularly after sample purification and concentration [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Different Extraction Approaches for the Analysis of Melatonin from Cabernet Sauvignon and Feteasca Neagra Wines Using a Validated HPLC-FL Method

et al. 2023

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In recent years, the wine industry has shown a considerable degree of interest in the occurrence of melatonin in wines. Sample pretreatment may be the most important step in trace analysis. Since wine is a complex matrix and melatonin is present in low amounts (ppb), an adequate extraction technique is required. In this study, the effect of several extraction methods, such as solid phase extraction (SPE), Quick, Easy, Cheap, Effective, Rugged, and Safe extraction (QuEChERS), and dispersive liquid–liquid micro-extraction (DLLME) was studied and the variable parameters that can arise throughout the extraction process were optimized to obtain the best results. A high-performance liquid chromatography with fluorescence detector (HPLC-FL) method was adapted and validated, including measurement uncertainty, for the analysis of melatonin in wines and to assess the efficiency of the extraction yield. After comparing the acquired results, the DLLME method was optimized. Extraction recoveries values ranging from 95 to 104% demonstrated that the approach may be successfully applied for the extraction and concentration (enrichment factor of almost eight) of melatonin in wine samples prior to HPLC-FL analysis. The first report of melatonin levels in Feteasca Neagra wines has been made. The data obtained for Cabernet Sauvignon revealed that the final levels of melatonin in the wines are dependent on the winemaking process.

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Section: Resultssupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Different Extraction Approaches for the Analysis of Melatonin from Cabernet Sauvignon and Feteasca Neagra Wines Using a Validated HPLC-FL Method

et al. 2023

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“…By increasing the dispersant’s volume, the extraction efficiency was gradually enhanced and finally reached a maximum at 900 μL. This may be due to that a small volume of ACN cannot fully disperse the extractant in the water phase, thus reducing the extraction efficiency [ 39 ]. Therefore, 900 μL of ACN was selected for dispersion.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Sulfonamides Antibiotics in Environmental Water and Seafood Samples Using Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography

Wang

Ji³

et al. 2022

Molecules

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The residues and abuse of antibiotics have seriously endangered ecological balance and human health; meanwhile, antibiotics determination is very difficult because of their low levels and multiple categories in complicated matrices. Appropriate sample pretreatment is usually imperative to enrich (ultra)trace antibiotics and eliminate matrix interference prior to chromatographic analysis. Dispersive liquid-liquid microextraction (DLLME) has become an ideal pretreatment technique owing to its simplicity, effectiveness, low-consumption, etc. In this work, an ultrasonic-assisted DLLME (UA-DLLME) was developed for the simultaneous extraction of seven sulfonamides (SAs) antibiotics in environmental water and seafood samples coupled with HPLC-DAD determination. Several parameters affecting UA-DLLME efficiency were systematically optimized, and consequently the SAs were separated and detected within 14.5 min. The obtained limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.7–7.8 μg/L and 2.4–26.0 μg/L for three water samples (seawater, aquaculture wastewater and lake water) and two seafood samples (pomfrets and shrimps). High recoveries (80.0–116.0%) with low relative standard deviations (0.1–8.1%) were achieved for all the tested samples at three spiked levels. Notably, sulfadimethoxine was found at 24.49 μg/L in one seawater sample. The facile, robust and benign DLLME-HPLC method demonstrated promising perspectives for multiresidue analysis of antibiotics.

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“…Besides phenolic compounds, melatonin has also been identified in wines, and its presence might be responsible for its role in putative wine health benefits [ 32 , 33 , 34 , 35 ].…”

Section: Wine Bioactive Compounds: Modulation Of Biochemical Pathways...mentioning

confidence: 99%

Moderate Wine Consumption and Health: A Narrative Review

et al. 2022

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Although it is clearly established that the abuse of alcohol is seriously harmful to health, much epidemiological and clinical evidence seem to underline the protective role of moderate quantities of alcohol and in particular of wine on health. This narrative review aims to re-evaluate the relationship between the type and dose of alcoholic drink and reduced or increased risk of various diseases, in the light of the most current scientific evidence. In particular, in vitro studies on the modulation of biochemical pathways and gene expression of wine bioactive components were evaluated. Twenty-four studies were selected after PubMed, Scopus and Google Scholar searches for the evaluation of moderate alcohol/wine consumption and health effects: eight studies concerned cardiovascular diseases, three concerned type 2 diabetes, four concerned neurodegenerative diseases, five concerned cancer and four were related to longevity. A brief discussion on viticultural and enological practices potentially affecting the content of bioactive components in wine is included. The analysis clearly indicates that wine differs from other alcoholic beverages and its moderate consumption not only does not increase the risk of chronic degenerative diseases but is also associated with health benefits particularly when included in a Mediterranean diet model. Obviously, every effort must be made to promote behavioral education to prevent abuse, especially among young people.

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Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD

Cited by 32 publications

References 34 publications

Different Extraction Approaches for the Analysis of Melatonin from Cabernet Sauvignon and Feteasca Neagra Wines Using a Validated HPLC-FL Method

Different Extraction Approaches for the Analysis of Melatonin from Cabernet Sauvignon and Feteasca Neagra Wines Using a Validated HPLC-FL Method

Simultaneous Determination of Sulfonamides Antibiotics in Environmental Water and Seafood Samples Using Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography

Moderate Wine Consumption and Health: A Narrative Review

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