Simultaneous determination of mecamylamine, nicotine, and cotinine in plasma by gas chromatography-mass spectrometry

Jacob, Peyton; Wu, Sylvia; Yu, Lisa; Benowitz, Neal L.

doi:10.1016/s0731-7085(00)00343-5

Cited by 50 publications

(36 citation statements)

References 8 publications

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“…Prior to each extraction, hexane, butanol, toluene, and water were each passed through a benzenesulfonic acid silica-gel cartridge (United Chemical Technologies), and glassware was heated in a muffle furnace at 500°C for 3 hr to remove background nicotine contamination. The liquid-liquid extraction protocol used for this analysis was adapted from Jacob, Wu, Yu, and Benowitz (2000). To a 0.1-g portion of dust in a centrifuge tube was added an internal standard (40 ng of methyl-d 3 -nicotine) and a 2-ml aqueous solution containing 45% potassium carbonate and 5% edetic acid, followed by sonication for 10 min.…”

Section: Laboratory Analysis Of Nicotinementioning

confidence: 99%

Levels of Nicotine in Dust From Homes of Smokeless Tobacco Users

Whitehead

Metayer

Park

et al. 2013

Nicotine & Tobacco Research

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Section: Laboratory Analysis Of Nicotinementioning

confidence: 99%

Levels of Nicotine in Dust From Homes of Smokeless Tobacco Users

Whitehead

Metayer

Park

et al. 2013

Nicotine & Tobacco Research

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“…Plasma nicotine levels were measured in duplicate using a gas chromatography-mass spectrometry method described by Jacob and co-workers (Jacob et al, 2000). The nicotine assay sensitivity was 5.0 ng/ml and the intra-and interassay CVs were 4.6 and 11.5%, respectively.…”

Section: Assay Proceduresmentioning

confidence: 99%

Effects of Smoking Successive Low- and High-Nicotine Cigarettes on Hypothalamic–Pituitary–Adrenal Axis Hormones and Mood in Men

Mendelson

Goletiani

Sholar

et al. 2007

Neuropsychopharmacol

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Smoking one cigarette produces rapid nicotine dose-related increases in hypothalamic-pituitary-adrenal (HPA) axis hormones, mood, and heart rate, but relatively little is known about the effects of smoking several cigarettes successively. Twenty-four healthy adult men who met Diagnostic and Statistical Manual of Mental Disorders, fourth edition (DSM-IV) criteria for nicotine dependence provided informed consent. After overnight abstinence from smoking, men smoked three low-or high-nicotine cigarettes for 4 min each at 60 min intervals. Samples for nicotine and hormone analysis, Visual Analog Scale (VAS) ratings of subjective effects and heart rate were collected at 4, 8, 12, 16, 20, 30, 40, and 50 min after each cigarette. After low-nicotine cigarettes, nicotine levels, adrenocorticotropin hormone, and heart rate did not increase significantly, cortisol and dehydroepiandrosterone decreased significantly, and positive VAS ratings were lower but parallel to ratings after high-nicotine cigarette smoking. After high-nicotine cigarettes, peak nicotine levels increased monotonically. HPA axis hormones increased after smoking, but peak levels did not differ significantly after successive high-nicotine cigarettes. Positive VAS ratings and heart rate increased after each high-nicotine cigarette, but peak levels were lower after smoking the second and third cigarette. 'Craving' decreased significantly after smoking both low-and high-nicotine cigarettes, then gradually increased during the 60 min interval between cigarettes. These data are consistent with clinical reports that the first cigarette after overnight nicotine abstinence is most salient. Tolerance to the subjective and cardiovascular effects of nicotine developed rapidly during repeated cigarette smoking, but nicotine-stimulated increases in HPA axis hormones did not change significantly.

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“…Plasma nicotine levels were measured in duplicate using a gas chromatography-mass spectrometry method described by Jacob et al (2000). The nicotine assay sensitivity was 1.0 ng/ml and the intra-and interassay CVs were 4.7 and 11.5%, respectively.…”

Section: Assay Proceduresmentioning

confidence: 99%

Effects of Low- and High-Nicotine Cigarette Smoking on Mood States and the HPA Axis in Men

Mendelson

Sholar

Goletiani

et al. 2005

Neuropsychopharmacol

125

106

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The acute effects of smoking a low-or high-nicotine cigarette on hypothalamic-pituitary-adrenal (HPA) hormones, subjective responses, and cardiovascular measures were studied in 20 healthy men who met American Psychiatric Association Diagnostic and Statistical Manual IV criteria for nicotine dependence. Within four puffs (or 2 min) after cigarette smoking began, plasma nicotine levels and heart rate increased significantly (Po0.01), and peak ratings of 'high' and 'rush' on a Visual Analogue Scale were reported. Reports of 'high,' 'rush,' and 'liking' and reduction of 'craving' were significantly greater after smoking a high-nicotine cigarette than a low-nicotine cigarette (Po0.05). Peak plasma nicotine levels after high-nicotine cigarette smoking (23.972.6 ng/ml) were significantly greater than after lownicotine cigarette smoking (3.6370.59 ng/ml) (Po0.001). After smoking a low-nicotine cigarette, adrenocorticotropin hormone (ACTH), cortisol, dehydroepiandrosterone (DHEA), and epinephrine did not change significantly from baseline. After high-nicotine cigarette smoking began, plasma ACTH levels increased significantly above baseline within 12 min and reached peak levels of 21.8875.34 pmol/l within 20 min. ACTH increases were significantly correlated with increases in plasma nicotine (r ¼ 0.85; Po0.0001), DHEA (r ¼ 0.66; P ¼ 0.002), and epinephrine (r ¼ 0.86; Po0.0001). Cortisol and DHEA increased significantly within 20 min (Po0.05) and reached peak levels of 424748 and 21.1372.55 ng/ml within 60 and 30 min, respectively. Thus cigarette smoking produced nicotine dose-related effects on HPA hormones and subjective and cardiovascular measures. These data suggest that activation of the HPA axis may contribute to the abuse-related effects of cigarette smoking.

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Simultaneous determination of mecamylamine, nicotine, and cotinine in plasma by gas chromatography-mass spectrometry

Cited by 50 publications

References 8 publications

Levels of Nicotine in Dust From Homes of Smokeless Tobacco Users

Levels of Nicotine in Dust From Homes of Smokeless Tobacco Users

Effects of Smoking Successive Low- and High-Nicotine Cigarettes on Hypothalamic–Pituitary–Adrenal Axis Hormones and Mood in Men

Effects of Low- and High-Nicotine Cigarette Smoking on Mood States and the HPA Axis in Men

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