Simultaneous determination of malondialdehyde and ofloxacin in plasma using an isocratic high-performance liquid chromatography/fluorescence detection system

Cheng, Guan-Wen; Wu, Hsin‐Lung; Huang, Yeou‐Lih

doi:10.1016/j.aca.2008.04.012

Cited by 38 publications

(19 citation statements)

References 28 publications

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“…Serum malondialdehyde (MDA) levels were measured by HPLC with fluorescence detection that quantifies genuine MDA-thiobarbituric acid adduct [18] avoiding the total absorbance of several species when using a spectrophotometric detection. Briefly, samples or standards (25 µl) were mixed with 0.44 M H 3 PO 4 (375 µl), 40 mM 2-thiobarbituric acid (125 µl) and ultrapure water (225 µl) and placed in a heating cabinet at 97 °C for 60 min.…”

Section: Assays and Chemicalsmentioning

confidence: 99%

A Mediterranean diet supplemented with extra virgin olive oil or nuts improves endothelial markers involved in blood pressure control in hypertensive women

et al. 2015

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Section: Assays and Chemicalsmentioning

confidence: 99%

A Mediterranean diet supplemented with extra virgin olive oil or nuts improves endothelial markers involved in blood pressure control in hypertensive women

et al. 2015

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“…The activity of the enzyme catalase (CAT) was determined by UV spectrophotometry (Cary 1E Varian), in accordance to the method described by Aebi (1984). High performance liquid chromatography (HPLC 1100 series Agilent Technologies) was used to quantify the malondialdehyde (MDA) levels (Cheng et al, 2008). Proteins were determined according to Bradford (1976) by Coomassie blue method, using bovine serum albumin as standard.…”

Section: Oxidative Stress Assaysmentioning

confidence: 99%

Assessment of mercury chloride-induced toxicity and the relevance of P2X7 receptor activation in zebrafish larvae

Cruz

Leite

Pereira

et al. 2013

Comparative Biochemistry and Physiology Part C: Toxicology &

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Zebrafish (Danio rerio) has been adopted as a model for behavioral, immunological and toxicological studies. Mercury is a toxic heavy metal released into the environment. There is evidence indicating that heavy metals can modulate ionotropic receptors, including the purinergic receptor P2X7. Therefore, this study evaluated the in vivo effects of acute exposure to mercury chloride (HgCl2) in zebrafish larvae and to investigate the involvement of P2X7R in mercury-related toxicity. Larvae survival was evaluated for 24 h after exposure to HgCl2, ATP or A740003. The combination of ATP (1 mM) and HgCl2 (20 μg/L) decreased survival when compared to ATP 1 mM. The antagonist A740003 (300 and 500 nM) increased the survival time, and reversed the mortality caused by ATP and HgCl2 in association. Quantitative real time PCR showed a decrease of P2X7R expression in the larvae treated with HgCl2 (20 μg/L). Evaluating the oxidative stress our results showed decreased CAT (catalase) activity and increased MDA (malondialdehyde) levels. Of note, the combination of ATP with HgCl2 showed an additive effect. This study provides novel evidence on the possible mechanisms underlying the toxicity induced by mercury, indicating that it is able to modulate P2X7R in zebrafish larvae.

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“…1(a)]. It is a broad spectrum antibiotic which belongs to the third generation of fluorinated quinolone (Awadallah et al, 2003;Elbashir et al, 2007;Zhou et al, 2006;Rizk et al, 1998;Gandhimathi et al, 2006;Shao et al, 2008) and is one of the most frequently used fluorinated quinolone antibiotics (Cheng et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of ofloxacin and ornidazole in pharmaceutical preparations by capillary zone electrophoresis

See

Elbashir

Saad

et al. 2009

Biomedical Chromatography

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A simple, rapid and validated capillary electrophoretic method has been developed for the separation and determination of ofloxacin and ornidazole in pharmaceutical formulations with detection at 230 nm. Optimal conditions for the quantitative separations were investigated. Analysis times shorter than 4 min were obtained using a background electrolyte solution consisting of 25 mmol/L phosphoric acid adjusted with 1 M Tris buffer to pH 8.5, with hydrodynamic injection of 5 s and 20 kV separation voltage. The validation criteria for accuracy, precision, linearity and limits of detection and quantitation were examined and discussed. An excellent linearity was obtained in concentration range 25-250 microg/mL. The detection limits for ofloxacin and ornidazole were 1.03 +/- 0.11 and 1.80 +/- 0.06 microg/mL, respectively. The proposed method has been applied for the analysis of ofloxacin and ornidazole both individually and in a combined dosage tablet formulation. The proposed validated method showed recoveries between 96.16 and 105.23% of the nominal contents.

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Simultaneous determination of malondialdehyde and ofloxacin in plasma using an isocratic high-performance liquid chromatography/fluorescence detection system

Cited by 38 publications

References 28 publications

A Mediterranean diet supplemented with extra virgin olive oil or nuts improves endothelial markers involved in blood pressure control in hypertensive women

A Mediterranean diet supplemented with extra virgin olive oil or nuts improves endothelial markers involved in blood pressure control in hypertensive women

Assessment of mercury chloride-induced toxicity and the relevance of P2X7 receptor activation in zebrafish larvae

Simultaneous determination of ofloxacin and ornidazole in pharmaceutical preparations by capillary zone electrophoresis

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