Simultaneous determination of losartan, losartan acid and amlodipine in human plasma by LC-MS/MS and its application to a human pharmacokinetic study

Karra, Vijaya Kumari; Pilli, Nageswara Rao; Inamadugu, Jaswanth Kumar; Rao, J. V. L. N. Seshagiri

doi:10.4103/2229-4708.97711

Cited by 33 publications

(22 citation statements)

References 23 publications

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“…The method was validated according to FDA guidelines and all parameters were in the accepted limits. The method was applied for human plasma for kinetic studies and the results were satisfactory [36]. Amlodipine in human plasma was analysed by Feng teamwork using LC-MS. Necardipine was used as internal standard and both drugs were extracted from plasma using ethyl acetate.…”

Section: 42mentioning

confidence: 99%

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Helaluddin¹,

Alaama²,

Sarker³

et al. 2017

J. CleanWAS

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Section: 42mentioning

confidence: 99%

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Helaluddin¹,

Alaama²,

Sarker³

et al. 2017

J. CleanWAS

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“…Karra et al 24 have reported a sensitive method for the simultaneous determination of LOS, EXP-3174, and amlodipine in human plasma. Moreover, the method was used for the pharmacokinetic analysis of only LOS and EXP-3174 in six healthy subjects.…”

Section: Methods Developmentmentioning

confidence: 99%

Simultaneous analysis of losartan, its active metabolite, and hydrochlorothiazide in human plasma by a UPLC-MS/MS method

Shah¹,

Sharma²,

Shah³

et al. 2015

Turk J Chem

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Abstract:A selective and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of losartan (LOS), EXP-3174, which is an active metabolite LOS carboxylic acid, and hydrochlorothiazide (HCTZ) in human plasma. Solid-phase extraction was carried out on Oasis HLB cartridges with 100 µ L of plasma to give an extraction recovery in the range of 88.5%-102.5% for the three analytes.Chromatography on a BEH C18 column afforded baseline separation of all the analytes within 2.4 min using 1.0% formic acid in water and acetonitrile (15:85, v/v) as the mobile phase. Quantitation was performed with multiple reaction monitoring in the negative ionization mode. The response of the method was linear over a dynamic range of 0.5-500, 1.0-750, and 0.25-150 ng/mL for LOS, EXP-3174, and HCTZ, respectively. Extent of signal suppression/enhancement was examined through postcolumn infusion. The effect of matrix components was evaluated by postextraction spiking and calculation of the slope of calibration lines. The method was successfully applied to a bioequivalence study of 50 mg losartan and 12.5 mg hydrochlorothiazide tablet formulation in 65 healthy human subjects. Reproducibility of the method was shown by reanalysis of 213 incurred samples.

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“…Capillary electrophoresis methods were also used for the analysis of AML in its formulations and biological fluids [15,16]. AML was also determined using liquid chromatography mass spectrometry (LC-MS) [17,18]. 1, 2-naphthoquinone-4-sulphonate (NQS) has been used as a chromogenic reagent for the colorimetric determination of many pharmaceutical amines [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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2017

JAPLR

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A rapid, simple and sensitive high-performance liquid chromatographic (HPLC) method for determination of Amlodipine (AML) using sodium 1,2-naphthoquinone-4-sulfonate (NQS) has been developed. The method is based on the formation of a brown color product from the reaction between AML and NQS. The nucleophilic substitution reaction proceeds quantitatively at pH 13 buffer solution with absorption maximum at 465 nm. A C 18 column ODS (250mm×4.6mm), 5μm and a mobile phase of combination is potassium dihydrogen orthophosphate (0.05M) in water: acetonitrile (45:55%v/v) were used for separation and quantification. Analyses were run at a flowrate of 1.5mL/min and at 40 °C temperature. The injection volume was 20μL and the photodiode array detector was set at 465nm. Under these conditions Amlodipine-NQS eluted at 3.12 min. Total run time was shorter than 5 min. The developed method was validated according to the literature and found to be linear within the range 2-30μg /mL, describes by the regression equation y =207,082x +1,481.71 with a regression coefficient 0.999. The limit of detection and quantity are 0.01μg/mL and 0.04μg∕ mL respectively. This method is simple and can be applied for the determination of AML in pharmaceutical formulation in quality control laboratories.

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Simultaneous determination of losartan, losartan acid and amlodipine in human plasma by LC-MS/MS and its application to a human pharmacokinetic study

Cited by 33 publications

References 23 publications

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Simultaneous analysis of losartan, its active metabolite, and hydrochlorothiazide in human plasma by a UPLC-MS/MS method

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