Simultaneous determination of linezolid and voriconazole serum concentrations using liquid chromatography-tandem mass spectrometry

A rapid and simple HPLC-MS/MS method for the therapeutic drug monitoring of six special-grade antimicrobials in pediatric patients

“…All TDM samples were collected before the next dose at a steady state (C min ). The recommended concentration ranges for meropenem, linezolid, fluconazole, voriconazole, posaconazole, and vancomycin were fT > MIC100 % [ 30 ], 2.0–7.0 μg/mL [ 15 ], at least 1.9–6.7 μg/mL [ 31 ], 1.0–5.5 μg/mL [ 15 ], 1–6 μg/mL [ 31 ], 10.0–20.0 μg/mL [ 12 ], respectively. As illustrated in Table 8 , C min of 6 special-grade antimicrobials varied considerably among individuals and many C min values were outside the recommended concentration ranges, including 36.4 % (8/22) of meropenem, 64.7 % (11/17) of linezolid, 33.3 % (1/3) of fluconazole, 66.7 % (8/12) of voriconazole, 41.2 % (7/17) of posaconazole, and 68.0 % (17/25) of vancomycin samples.…”

“…Several LC–MS/MS methods have been published to determine different antibacterials, summarized in Table 9 and compared with our method. Most reported methods usually targeted the same class of antimicrobials, e.g., beta-lactams or azoles [ [11] , [12] , [13] , [14] , [15] , [16] ]. This means that several methods should be applied in the routine TDM to cover different antimicrobials mentioned in this article.…”

“…In recent years, liquid chromatography-tandem mass spectrometry (LC–MS/MS) has been used more commonly in TDM due to its high selectivity, sensitivity, and throughput [ 10 ]. Some LC–MS/MS assays have also been published to quantify different antimicrobials [ [11] , [12] , [13] , [14] , [15] , [16] ]. However, the methods usually combine antimicrobials belonging to the same class, and none of them includes the six analytes simultaneously.…”

The Value of Liquid Chromatography-Mass Spectrometry in the Detection of Chemical Constituents of Kidney Tonic and Aphrodisiac Chinese Herbal Preparations

“…(4) Centrifuge: Used for centrifugal separation of solid particles and liquids in drugs and samples. (5) Oven: Used to dry the samples or reagents. (6) pH meter: Used to determine the acid-base properties of drugs or reagents.…”

“…Conventional chemical analysis methods can be limited by sample complexity and low concentration of components, whereas liquid-mass spectrometry can overcome these difficulties and provide highly sensitive, selective, and attributable chemical analysis results. Liquid chromatography-mass spectrometry (LC-MS) combines chromatographic separation and mass spectrometry detection technologies to achieve accurate identification and quantitative analysis of multiple chemical components in complex samples, with the advantages of high sensitivity, accuracy, and throughput [5] . In kidney tonic and aphrodisiac herbal preparations, LC-MS can help identify and quantify various active ingredients, such as phytosterols, alkaloids, flavonoids, polysaccharides, and peptides.…”