Simultaneous determination of key bioactive components in Hedyotis diffusa by capillary electrophoresis

Cheung, Hon-Yeung; Cheung, Shilin; Law, Mark; Lai, Wai-Ping

doi:10.1016/j.jchromb.2006.02.007

Cited by 17 publications

(10 citation statements)

References 12 publications

(10 reference statements)

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“…Hon-Yeung CHEUNG is the first scientist reporting on this compound, which is found in Hedyotic diffusa Willd. and Prunell avulgaris L. (Cheung et al, 2006;Cheung and Zhang, 2008). It exhibits a broad range of pharmacological effects such as anti-inflammatory, antiviral, antioxidant, and hepatoprotective activities (Feuillolay et al, 2016;Kashyap et al, 2016;Siegel et al, 2016), and has proven to be effective in treating various types of cancers including CRC (Pathak et al, 2007;Prasad et al, 2012;Lin et al, 2013;Kadioglu and Efferth, 2015).…”

Section: Introductionmentioning

confidence: 99%

Ursolic acid sensitized colon cancer cells to chemotherapy under hypoxia by inhibiting MDR1 through HIF-1α

Shan

Xuan

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To explore the efficacy of ursolic acid in sensitizing colon cancer cells to chemotherapy under hypoxia and its underlying mechanisms. Methods: Three colon cancer cell lines (RKO, LoVo, and SW480) were used as in vitro models. 5-Fluorouracil (5-FU) and oxaliplatin were used as chemotherapeutic drugs. Cell viability and apoptosis were tested to evaluate the sensitivity of colon cancer cells to chemotherapy. The transcription and expression levels of hypoxia-inducible factor-1α (HIF-1α), multidrug resistance gene 1 (MDR1), and vascular endothelial growth factors (VEGF) were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunoblotting. Cycloheximide and MG132 were used to inhibit protein synthesis and degradation, respectively. In vitro tube formation assay was used to evaluate angiogenesis. Results: We demonstrated the chemosensitizing effects of ursolic acid with 5-FU and oxaliplatin in three colon cancer cell lines under hypoxia. This effect was correlated to its inhibition of MDR1 through HIF-1α. Moreover, ursolic acid was capable of inhibiting HIF-1α accumulation with little effects on its constitutional expression in normoxia. In addition, ursolic acid also down-regulated VEGF and inhibited tumor angiogenesis. Conclusions: Ursolic acid exerted chemosensitizing effects in colon cancer cells under hypoxia by inhibiting HIF-1α accumulation and the subsequent expression of the MDR1 and VEGF.

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Section: Introductionmentioning

confidence: 99%

Ursolic acid sensitized colon cancer cells to chemotherapy under hypoxia by inhibiting MDR1 through HIF-1α

Shan

Xuan

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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“…In China, HDW is used as cancer therapy agent, which improves the adverse reactions of chemotherapy Lin et al, 2013b). The studies of molecular pharmacology showed that HDW contains major compounds with anti-cancer activities, including anthraquinones (Liu et al, 2010;Wang et al, 2011;Niu and Meng, 2013), polyphenols (Han et al, 1997), organic acids (Cheung et al, 2006;Huang et al, 2009), hemiterpenes (Wang et al, 2012;Niu and Meng, 2013), and polysaccharides (Cui et al, 2006;Lin et al, 2013c). Several reports showed that the extract of HDW induced cell cycle and apoptosis in many human cancer cell lines (Liu et al, 2010;Lin et al, 2011bLin et al, , 2012Zhang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Ethanol extract ofHedyotis diffusawilld upregulates G0/G1 phase arrest and induces apoptosis in human leukemia cells by modulating caspase cascade signaling and altering associated genes expression was assayed by cDNA microarray

Kuo

Yang

et al. 2014

Environ. Toxicol.

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The authors' previous study has shown that water extract of Hedyotis diffusa Willd (HDW) promoted immune response and exhibited anti-leukemic activity in BALB/c leukemic mice in vivo. In this study, the anti-proliferation effects of ethanol extract of H. diffusa Willd (EEHDW) on lung cancer cell lines (A549, H1355, and LLC), leukemia cell lines (HL-60, WEHI-3), and a mouse melanoma cell line (B16F10) in vitro were investigated. The results demonstrated that EEHDW suppressed the cell proliferation of A549, H1355, HL-60, WEHI-3, and B16F10 cells as well as reduced cell viability in a concentration-dependent manner. We found that EEHDW inhibited the cell proliferation of HL-60 cells in concentration-dependent manner. In addition, EEHDW triggered an arrest of HL-60 cells at G0/G1 phase and sub-G1 population (apoptotic cells). EEHDW provoked DNA condensation and DNA damage in HL-60 cells. The activities of caspase-3, caspase-8, and caspase-9 were elevated in EEHDW-treated HL-60 cells. DNA microarray to investigate and display the gene levels related to cell growth, signal transduction, apoptosis, cell adhesion, cell cycle, DNA damage and repair, transcription and translation was also used. These findings suggest that EEHDW may be a potential herbal medicine and therapeutic agent for the treatment of leukemia.

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“…Cheung et al developed a capillary zone electrophoresis method for simultaneous determination of four compounds (geniposidic acid, ursolic acid, quercetin and p-coumaric acid) in the extract of H. diffusa [16]. Recently, the HPLC-MS E-Hu Liu 1 Ting Zhou 1 Guo-Bin Li 1 Jing Li 1 fingerprint method has been developed for different batches of H. diffusa, but the study only dealt with three compounds including oleanolic acid, ursolic acid and ferulic acid [17].…”

Section: Introductionmentioning

confidence: 99%

Characterization and identification of iridoid glucosides, flavonoids and anthraquinones in Hedyotis diffusa by high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry

Liu

Zhou

et al. 2012

J of Separation Science

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The multiple bioactive constituents in Hedyotis diffusa Willd. (H. diffusa) were extracted and characterized by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS(n)). The optimized separation condition was obtained using an Agilent ZorBax SB-C18 column (4.6×150 mm, 5 μm) and gradient elution with water (containing 0.1% formic acid) and acetonitrile (containing 0.1% formic acid), under which baseline separation for the majority of compounds was achieved. Among the compounds detected, 14 iridoid glucosides, 10 flavonoids, 7 anthraquinones, 1 coumarin and 1 triterpene were unambiguously identified or tentatively characterized based on their retention times and mass spectra in comparison with the data from standards or references. The fragmentation behavior for different types of constituents was also investigated, which could contribute to the elucidation of these constituents in H. diffusa. The present study reveals that even more iridoid glycosides were found in H. diffusa than hitherto assumed. The occurrence of two iridoid glucosides and five flavonoids in particular has not yet been described. This paper marks the first report on the structural characterization of chemical compounds in H. diffusa by a developed HPLC-ESI-MS(n) method.

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Simultaneous determination of key bioactive components in Hedyotis diffusa by capillary electrophoresis

Cited by 17 publications

References 12 publications

Ursolic acid sensitized colon cancer cells to chemotherapy under hypoxia by inhibiting MDR1 through HIF-1α

Ursolic acid sensitized colon cancer cells to chemotherapy under hypoxia by inhibiting MDR1 through HIF-1α

Ethanol extract ofHedyotis diffusawilld upregulates G0/G1 phase arrest and induces apoptosis in human leukemia cells by modulating caspase cascade signaling and altering associated genes expression was assayed by cDNA microarray

Characterization and identification of iridoid glucosides, flavonoids and anthraquinones in Hedyotis diffusa by high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry

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