Simultaneous determination of isoxaflutole and its two metabolites in corn under field conditions by LC–MS/MS

Abstract

OBJECTIVE An improved QuEChERS method was established and verified for simultaneous determination of isoxaflutole and its metabolites diketonitrile and benzoic acid analogue residues in corn and plants. This method was mainly used to study the digestion rule and final residue level of isoxaflutole and its metabolites in corn and plants. It was hoped that the safe use of isoxaflutole in corn can be achieved eventually. METHOD The method was completed by means of ultra‐performance liquid chromatography with triple quadrupole mass spectrometry. The extraction of the target substance was through acetonitrile solution containing 1% acetic acid, and the purification was through primary secondary amine, octadecylsilane and graphitized carbon black sorbent. RESULTS In the method, the quantitative limits and detection limits of the three analytes were 0.005–0.01 and 0.001–0.003 mg kg−1 respectively. The half‐life of isoxaflutole in the plants in Shandong and Anhui was 36.4 and 42.1 days respectively, and the digestion dynamics all conformed to the first‐order kinetics. The final residues of isoxaflutole in green corn and mature corn were less than 0.02 mg kg−1 of the maximum residue limit set by the Codex Alimentarius Commission. CONCLUSIONS The residual amount of isoxaflutole in corn and plants at harvest time was acceptable when isoxaflutole was applied once at a dose of 121.5 g a.i. ha−1. © 2021 Society of Chemical Industry.

Ecotoxicological Evaluation of Safener and Antimicrobial Additives in Isoxaflutole-Based Herbicide Formulations

“…For the determination of isoxaflutole at low concentrations, liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is the analytical method of choice with LOD and LOQ values low enough to detect the a.i. under the maximum residue level in food and feed products or the maximum individual limit (0.1 μg/L) in surface water [ 92 , 93 , 94 , 95 ]. In our study, a simpler LC-UV method was applied for the detection of isoxaflutole dissipation, as our aim was only to determine its DT 50 values, not the a.i.…”

Terminal Residue and Dietary Risk Assessment of Atrazine and Isoxaflutole in Corn Using High-Performance Liquid Chromatography–Tandem Mass Spectrometry

“…Lin et al [6] developed high-performance liquid chromatography-UV (HPLC-UV) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the analysis of IFT and its two metabolites, IFT-DKN and benzoic acid metabolite (BA) in soil, water and plant samples [22,23]. Lan et al used modified QuEChERS pre-treatment and high-performance liquid chromatography-tandem mass spectrometry to determine isoxazole and its metabolites in corn and straw, and the halflives of IFT in Shandong and Anhui provinces were 36.4 and 42.1 days, respectively [24].…”