Simultaneous determination of isomeric substituted anilines by imidization with benzaldehyde and gas chromatography–mass spectrometry

Abstract

The chromatographic separation of several isomeric anilines is a challenging issue. Herein, a simple method for the simultaneous determination of four groups of isomeric primary aromatic amines, including chloroanilines, methylanilines, methoxylanilines, and dimethylanilines, was presented. In this method, all of the 15 primary aromatic amines were easily transformed into the corresponding imine derivative by treatment with benzaldehyde under mild conditions. The formed isomeric imine derivatives were completely separated on a commercial capillary gas chromatography column. The effects of several derivatization parameters were investigated and optimized. Linearity in the optimized method ranged from 0.050 to 50 μg/mL with the squared correlation coefficients (R ) between 0.9981 and 0.9999. Reasonable reproducibility was obtained, with the intraday relative standard deviation (N = 5) ranging from 0.89 to 4.57% and interday relative standard deviation ranging from 2.26 to 7.69% at the concentration of 5.0 μg/mL. The developed method has been successfully applied to determine these isomeric aromatic amines in real samples.

Amphiphilic Block Copolymer PCL-PEG-PCL as Stationary Phase for Capillary Gas Chromatographic Separations

“…All halogenated benzene isomers, especially the critical pairs of m -/ p -dichlorobenzene and 2,4-dichlorobenzaldehyde/2,5-dichlorobenzaldehyde with boiling points difference less than 1 °C, were completely resolved, probably owing to their slightly differences in H-bonding and dipole–dipole interactions with the stationary phase. Toluidine isomers are important materials in chemical industry but they are also the known critical isomers in GC [36,37]. Besides, o -toluidine is listed in 24 types of carcinogenic aromatic amine by the European Union [38].…”

An Amphiphilic Star-Shaped Polymer (Star-PEG-PCL2) used as a Stationary Phase for GC

“…The high toxicity and carcinogenicity of aromatic amines pose a great threat to humans and the environment, so the separation of aromatic amine isomers is extremely important. [34][35][36] The separation of aromatic amines is a challenge in the field of GC analysis. Fig.…”

Star-poly(ε-caprolactone) as the stationary phase for capillary gas chromatographic separation

“…The affective separation of aniline isomers is a challenge in the analysis eld, and GC is a common analytic method. 50,51 However, aniline isomers oen have to be derivated for effective separation, which is caused by the close physicochemical properties. As shown in Fig.…”