Simultaneous Determination of Inorganic Cations and Anions in Microchip Electrophoresis Using High-voltage Relays

Huang, Zhenguo; Yang, Mingpeng; You, Hui; Xie, Yang

doi:10.2116/analsci.18p022

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…This voltage signal was then lock-in amplified [ 31 ], filtered and gained, and finally sampled by an acquisition unit as shown in Figure 2 . The high-voltage modules and associated circuits used in the system have been described in the previous work [ 32 ]. The data acquisition was done by a data acquisition unit (MAX194, Maxim Integrated, San Jose, CA, USA) and a software written in LABVIEW (National Instruments, Austin, TX, USA).…”

Section: Methodsmentioning

confidence: 99%

A Novel Planar Grounded Capacitively Coupled Contactless Conductivity Detector for Microchip Electrophoresis

Wang

Liu

et al. 2022

Micromachines

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In the microchip electrophoresis with capacitively coupled contactless conductivity detection, the stray capacitance of the detector causes high background noise, which seriously affects the sensitivity and stability of the detection system. To reduce the effect, a novel design of planar grounded capacitively coupled contactless conductivity detector (PG-C4D) based on printed circuit board (PCB) is proposed. The entire circuit plane except the sensing electrodes is covered by the ground electrode, greatly reducing the stray capacitance. The efficacy of the design has been verified by the electrical field simulation and the electrophoresis detection experiments of inorganic ions. The baseline intensity of the PG-C4D was less than 1/6 of that of the traditional C4D. The PG-C4D with the new design also demonstrated a good repeatability of migration time, peak area, and peak height (n = 5, relative standard deviation, RSD ≤ 0.3%, 3%, and 4%, respectively), and good linear coefficients within the range of 0.05–0.75 mM (R2 ≥ 0.986). The detection sensitivity of K+, Na+, and Li+ reached 0.05, 0.1, and 0.1 mM respectively. Those results prove that the new design is an effective and economical approach which can improve sensitivity and repeatability of a PCB based PG-C4D, which indicate a great application potential in agricultural and environmental monitoring.

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Section: Methodsmentioning

confidence: 99%

A Novel Planar Grounded Capacitively Coupled Contactless Conductivity Detector for Microchip Electrophoresis

Wang

Liu

et al. 2022

Micromachines

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“…A variety of analytical methods have been employed to measure potassium levels. These include atomic absorption spectroscopy (AAS) (Ieggli et al, 2010), inductively coupled plasma mass spectroscopy (ICP-MS) (Paulauskas et al, 2020;Yan et al, 2017), ion chromatography (Caland et al, 2012), electrophoresis (Huang et al, 2018), and electrochemical methods (Sedenho et al, 2013). Although these methods provide high sensitivity and resolution, they require expensive equipment, large amounts of chemicals, and a trained technician.…”

Section: Introductionmentioning

confidence: 99%

An Indirect Colorimetric Method for Potassium Determination in Soil Using a Paper Device and Smartphone

Thongkam

Hemavibool²

2022

molekul

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This study presents a simple method for determination of potassium in microliter scale using a paper device together with a smartphone. The method begins with the ion-pair extraction of dibenzo-18-crown-6-K+ complex into dichloromethane with an excess amount of calmagite. The aqueous phase containing the remaining calmagite is transferred to the paper device, where a smartphone is used to capture the color and convert to RGB value. The linear detection range was found to cover potassium concentrations from 20 mg L-1 to 120 mg L-1. The detection and quantification are 5.41 mg L-1 and 18.03 mg L-1, respectively. Potassium detection was carried out in a variety of actual soil samples, and the results were validated against spectrophotometric results using a paired t-test, which indicated high accuracy. The proposed method is simple, fast, and inexpensive, and it requires no complicated equipment, making it ideally suited for detection of potassium in soil.

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“…These include pressureassisted separation, 14 dual opposite-end injection, 15,16 dual single-end injection, 17 dual-channel capillary electrophoresis, 18 and high-voltage switching in microchip electrophoresis. 19 These methods have high resolution but some require specific instrumentation or two detectors for concurrent determination of cations and anions in a single run.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Inorganic Anions and Cations in Water and Biological Samples by Capillary Electrophoresis with a Capacitive Coupled Contactless Conductivity Detector Using Capillary Filling Method

et al. 2018

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An analytical method for concurrent analysis of inorganic anions and cations has been developed using a capillary electrophoresis (CE)-capacitively coupled contactless conductivity detector (C 4 D) system. Although hydrodynamic and electrokinetic injection techniques have been widely used in CE, we employed a capillary filling method (CFM) for the analysis of inorganic ions. The procedure is relatively simple and has the advantage that CMF does not require pressure control and vial exchange. Three anions (chloride, sulfate, nitrate) and five cations (ammonium, potassium, sodium, magnesium, calcium) were successfully separated and detected at ppm levels within 80 s using a 9 mM histidine/15 mM malic acid (pH 3.6) containing 50 mM N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate as background electrolyte. Applying this analytical condition, the electroosmotic flow is negligible and anions and cations were migrated concurrently to different polarities according to their electrophoretic mobility. Obtained raw data showed stepwise increases in detected conductivity due to the migration of sample components, which expresses as peak profiles by differentiation of electropherograms. The RSD values of the peak area and migration times for the anions and cations were satisfactory and were less than 5.15 and 2.04%, respectively. The developed method was applied for the analysis of inorganic anions and cations in commercial mineral waters, tap water, urine, and exhaled breath condensate. These results indicate that the CE-C 4 D system with CFM is suitable for the rapid analysis of inorganic anions and cations in various samples. Keywords Capillary electrophoresis (CE), capacitively coupled contactless conductivity detector (C 4 D), capillary filling method (CMF), water analysis

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Simultaneous Determination of Inorganic Cations and Anions in Microchip Electrophoresis Using High-voltage Relays

Cited by 9 publications

References 19 publications

A Novel Planar Grounded Capacitively Coupled Contactless Conductivity Detector for Microchip Electrophoresis

A Novel Planar Grounded Capacitively Coupled Contactless Conductivity Detector for Microchip Electrophoresis

An Indirect Colorimetric Method for Potassium Determination in Soil Using a Paper Device and Smartphone

Simultaneous Determination of Inorganic Anions and Cations in Water and Biological Samples by Capillary Electrophoresis with a Capacitive Coupled Contactless Conductivity Detector Using Capillary Filling Method

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