Simultaneous determination of imipramine, desipramine and their 2- and 10-hydroxylated metabolites in human plasma and urine by high-performance liquid chromatography

Urine sample preparation of tricyclic antidepressants by means of a supported liquid membrane technique for high-performance liquid chromatographic analysis

“…This method used simple E-mail address: trocew@hermes.umcs.lublin.pl (J. Trocewicz). liquid-liquid extraction and achieved the detection limit about 4 ng/ml, what is similar to results obtained by Chen et al [2]. Forensic analysis of 11 tricyclic antidepressants in human biological samples was described by Tanaka et al [3].…”

“…The presented method permits analysis of tricyclic antidepressants in biological fluids at very low (ng/ml) range without additional derivatization procedures or applying complicated procedures [2,3] or expensive instrumentation [5].…”

Sensitive quantification of chosen drugs by reversed-phase chromatography with electrochemical detection at a glassy carbon electrode

“…It is frequently used as an antiemetic and for the treatment of gut mobility disorders [9]. Imipramine hydrochloride (10,11- 1B) remains one of the most widely used drugs of tricyclic antidepressants class, which has been the mainstay of treatment for major depression for over 30 years [10]. Diclofenac potassium (potassium o-(2,6-dichlorophenyl)aminophenylacetate) (Fig.…”

“…Only one method with liquid-liquid extraction has been described for the determination of hydrochlorothiazide from human serum using electrochemical detection [71]. Moreover, a chemical oxidation step of imipramine [2,10,72] as well as diclofenac potassium [73,74] is needed in order to improve the analytical sensitivity, hence, electrochemical methods for the determination of these drugs were utilized. However, it was found during the development of the method that the injections were not reproducible.…”

Validation of non-aqueous capillary electrophoresis for simultaneous determination of four tricyclic antidepressants in pharmaceutical formulations and plasma samples

“…The applicability of TDM depends on the availability of rapid, sensitive, and reliable methods for drug determination. The main technique used in TAD analysis is high performance liquid chromatography (HPLC), which requires specific stationary phases besides the use of relatively large amounts of organic solvents [5][6][7]. Capillary electrophoresis (CE) is a complementary analytical technique to HPLC.…”