Simultaneous Determination of Hydrochlorothiazide and Losartan Potassium in Pharmaceutical Product by UV-Vis Spectrophotometric Method with Kalman Filter Algorithm

Abstract

In this paper, hydrochlorothiazide (HCT) and losartan potassium (LSP) in tablets are simultaneously determined with UV-Vis spectrophotometry and chemometrics without separation. The spectra of standard and sample solutions were recorded in the wavelength from 220 to 300 nm at 1.0 nm intervals. The concentrations of HCT and LSP in the sample solutions were computed with the Kalman filter algorithm written on the Microsoft Excel 2016 and Visual Basic for Applications (VBA) platform. The method validation was determined via the accuracy and repeatability of measurements when analyzing HCT and LSP in the Splozarsin Plus tablet and comparing the mean values of their contents in the sample with those analyzed with HPLC. The proposed method is simple with a low cost compared with the standard HPLC method.

A Rapid DMeyer-CWT Method Application to the Spectrophotometric Data for the Quantification of Losartan Potassium and Hydrochlorothiazide in a Binary Mixture

“…The aim of this study is to propose a new signal processing approach based on the simultaneous quantitative detection of losartan potassium (LOS) and hydrochlorothiazide (HCT) in tablets without the usage of a separation step using CWT and zero crossing methodology [15]. In pharmacological and biological investigations, several analytical techniques for the determination of LOS and HCT have been published, including spectrophotometric methods [16][17][18][19], spectrofluorometric methods [20], and chromatographic methods [21][22][23][24][25].…”

Spectrophotometric Determination of Losartan Potassium and Hydrochlorothiazide in tablets by Wavelet Transform Approach

“…In this paper, the aim is is to develop new signal processing method based on the combined use of CWT with zero crossing technique simultaneous quantitative determination of losartan potassium (LOS) and hydrochlorothiazide (HCT) in tablets without the use of a separation step. Several analytical methods for the analysis of LOS and HCT have been reported in pharmaceutical and biological studies, including spectrophotometric methods [15][16][17][18], spectrofluorometric method [19] and chromatographic methods [20][21][22][23][24].…”