Simultaneous Determination of Heavy Metals in Bovine Liver and Oyster Tissue by Solvent Extraction-Reversed Phase High Performance Liquid Chromatography

Separation and simultaneous determination of aluminum, iron, and manganese in natural water samples by using high-performance liquid chromatography with spectrophotometric and electrochemical detection

“…In the past decade, a number of reports have appeared on the reversed-phase high-performance liquid chromatographic (RPHPLC) separation and determination of trace metals after precolumn derivatizing with the use of bonded stationary phase (1)(2)(3)(4)(5). The most frequently used derivatizing agents include dithiocarbamic acids (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18) and related compounds (19)(20)(21), 8-quinolinol (22)(23)(24), /3-diketones (25), and 4-(2pyridylazo) resorcinol (26)(27)(28)(29)(30)(31)(32)(33).…”

Simultaneous determination of heavy metals in Chlorella and tea leaves by high-performance liquid chromatography

“…Heavy-metal contaminants in ammonium citrate solution (1 M, pH 9) were removed by extracting with a dithizone-chloroform solution. Hexamethyleneammonium hexamethylenedithiocarbamate (HMA-HMDC) was synthesized according to a procedure described in a previous paper (Ichinoki et al, 1984). All solvents used were of LC grade.…”

“…The liquid chromatograph used in this study consisted of a Model KHP-010 pump (Kyowa Seimitsu Co. Ltd., Tokyo, Japan), a Model UVIDEC-100-III variable-wavelength detector (Japan Spectroscopic Co., Tokyo, Japan), and a Model C-R1B data processor (Shimadzu Co., Kyoto, Japan). Eluted metal chelates were detected at 260 nm (Chlorella) or 290 nm (tea leaves, sensitive for nickel; Ichinoki et al, 1984). The stainless steel column (4.6 mm (i.d.)…”

“…Under these conditions, nanogram/milliliter levels of Cd, Ni, Pb, Zn, Cu, Hg, Co, and Bi may be extracted simultaneously. Details of the extraction of HMDC chelates into chloroform were described by Ichinoki et al (1984).…”

“…In HPLC procedures for heavy metals, dithiocarbamates have been used as chelating agents (Edward-Inatimi, 1983; Bond and Wallace, 1984;Smith et al, 1985). Unfortunately, there have been very few applications of this procedure to samples other than water (Edward-Inatimi, 1983; Ichinoki et al, 1984;Ichinoki and Yamazaki, 1985). A pretreatment method (ashing or extraction) suitable for one type of sample is not always suitable for another due to different matrices present in the samples.…”