Simultaneous determination of ginkgolides A, B, C and bilobalide in plasma by LC–MS/MS and its application to the pharmacokinetic study of Ginkgo biloba extract in rats

Xie, Jiashu; Ding, Cungang; Qing-hua, GE; Zhou, Zhen; Xiao-jin, Zhi

doi:10.1016/j.jchromb.2008.01.045

Cited by 47 publications

(34 citation statements)

References 12 publications

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“…Liquid chromatography-mass spectrometry (LC-MS) is an effective solution for complex matrix samples and is often used to study pharmacokinetics because of its high sensitivity and specificity. Xie et al (2008) and Jin et al (2011) successfully used an HPLC-tandem mass spectrometry (MS/MS) method to study the pharmacokinetics of terpene lactones. However, most methods usually focus only on flavonoid compounds or terpene lactones separately.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study

Wang

Liu

Kang

et al. 2014

J. Zhejiang Univ. Sci. B

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A rapid, reliable, and sensitive method was developed using ultra-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS) with an electrospray ionization (ESI) source for determination of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets (GBTs). The method simultaneously detects bilobalide (BB), ginkgolide A (GA), ginkgolide B (GB), ginkgolide C (GC), quercetin (QCT), kaempferol (KMF), and isorhamnetin (ISR) for pharmacokinetic study. The analytes and internal standard (IS) were extracted from rat plasma by acetidin. An MS/MS detection was conducted using multiple reaction monitoring (MRM) and operating in the negative ionization mode. The calibration curve ranges were 5-500, 5-500, 2.5-250, 1-100, 1-100, 1-100, and 1-100 ng/ml for BB, GA, GB, GC, QCT, KMF, and ISR, respectively. The mean recovery of the analytes ranged from 68.11% to 84.42%. The intra-and inter-day precisions were in the range of 2.33%-9.86% and the accuracies were between 87.67% and 108.37%. The method was used successfully in a pharmacokinetic study of GBTs. The pharmacokinetic parameters of seven compounds were analyzed using a non-compartment model. Plasma concentrations of the seven compounds were determined up to 48 h after administration, and their pharmacokinetic parameters were in agreement with previous studies.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study

Wang

Liu

Kang

et al. 2014

J. Zhejiang Univ. Sci. B

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“…It is mainly cultivated for its nuts and leaves. The most important constituents of standardized extracts of dried G. biloba leaves are flavone glycosides (quercetin, kaempferol, and isorhamnetin) and terpene lactones (ginkgolides and bilobalide) [51,52]. A standardized and popular commercial G. biloba extract called EGb 761 contains various biologically active constituents.…”

Section: Ginkgo Bilobamentioning

confidence: 99%

Traditional Korean East Asian Medicines and Herbal Formulations for Cognitive Impairment

Kumar

Song

et al. 2013

Molecules

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Abstract:Hanbang, the Traditional Korean Medicine (TKM), is an inseparable component of Korean culture both within the country, and further afield. Korean traditional herbs have been used medicinally to treat sickness and injury for thousands of years. Oriental medicine reflects our ancestor's wisdom and experience, and as the elderly population in Korea is rapidly increasing, so is the importance of their health problems. The proportion of the population who are over 65 years of age is expected to increase to 24.3% by 2031. Cognitive impairment is common with increasing age, and efforts are made to retain and restore the cognition ability of the elderly. Herbal materials have been considered for this purpose because of their low adverse effects and their cognitive-enhancing or anti-dementia activities. Herbal materials are reported to contain several active compounds that have effects on cognitive function. Here, we enumerate evidence linking TKMs which have shown benefits in memory improvements. Moreover, we have also listed Korean herbal formulations which have been the subject of scientific reports relating to memory improvement.

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“…Other authors used APCI interface combined with a triple quadrupole analyzer for investigating the bioavailability of ginkgolides after intravenous administration to rats [Xie et al, 2008] Specifically, multiple reaction monitoring (MRM) was used and the limit of quantification resulted around 2 ng/mL. In alternative to APCI, Hua et al (2006) proposed electrospray interface coupled to Q-arrayOctapole-Quadrupole mass analyzer (QoQ) for investigating the intragastric administration of pure ginkgolide B (0.1 ng/kg) in Beagle dogs.…”

Section: Pharmacokinetic Analysismentioning

confidence: 99%

Analytical Methods for Characterizing Bioactive Terpene Lactones in Ginkgo Biloba Extracts and Performing Pharmacokinetic Studies in Animal and Human

Rossi¹,

Basilico²,

Palma³

et al. 2011

Biomedical Engineering, Trends, Research and Technologies

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Simultaneous determination of ginkgolides A, B, C and bilobalide in plasma by LC–MS/MS and its application to the pharmacokinetic study of Ginkgo biloba extract in rats

Cited by 47 publications

References 12 publications

Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study

Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study

Traditional Korean East Asian Medicines and Herbal Formulations for Cognitive Impairment

Analytical Methods for Characterizing Bioactive Terpene Lactones in Ginkgo Biloba Extracts and Performing Pharmacokinetic Studies in Animal and Human

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