Simultaneous determination of gentisic, salicyluric and salicylic acid in human plasma using solid-phase extraction, liquid chromatography and electrospray ionization mass spectrometry

Pirker, R.; Huck, Christian W.; Popp, Michael; Bonn, G. K

doi:10.1016/j.jchromb.2004.06.031

Cited by 12 publications

(6 citation statements)

References 26 publications

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“…Actual plasma concentration of parent drug with metabolites is of therapeutic importance, although the prodrug is not active. Numerous methods are available for detection of ASA with its active metabolite salicylic acid (SA) (Altamura et al , ; Bae et al , ; McMahon and Kelly, ; Mullangi et al , ; Pirker et al , ; Shaw and Tsai, ; Yamamoto et al , ). Similarly, various methods are available for determination of CLP and CLPM (Delavenne et al , , ; El‐Sadek et al , ; Karaźniewicz‐Łada et al , ; Peer et al , ; Robinson et al , ; Silvestro et al , ; Singh et al , ; Souri et al , ; Yang et al , ) However, owing to rapid and extensive metabolism, ASA and CLP were found at very low concentration in plasma (pg/mL).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation of acetylsalicylic acid and clopidogrel along with their metabolites in human plasma using liquid chromatography tandem mass spectrometry

Chhonker

Pandey

Chandasana

et al. 2015

Biomedical Chromatography

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The interest in therapeutic drug monitoring has increased over the last few years. Inter- and intra-patient variability in pharmacokinetics, plasma concentration related toxicity and success of therapy have stressed the need of frequent therapeutic drug monitoring of the drugs. A sensitive, selective and rapid liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous quantification of acetylsalicylic acid (aspirin), salicylic acid, clopidogrel and carboxylic acid metabolite of clopidogrel in human plasma. The chromatographic separations were achieved on Waters Symmetry Shield(TM) C18 column (150 × 4.6 mm, 5 µm) using 3.5 mm ammonium acetate (pH 3.5)-acetonitrile (10:90, v/v) as mobile phase at a flow rate of 0.75 mL/min. The present method was successfully applied for therapeutic drug monitoring of aspirin and clopidogrel in 67 patients with coronary artery disease.

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Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation of acetylsalicylic acid and clopidogrel along with their metabolites in human plasma using liquid chromatography tandem mass spectrometry

Chhonker

Pandey

Chandasana

et al. 2015

Biomedical Chromatography

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“…The method described herein has an advantage in the specificity to immunoassay. Assays of salicylate are also carried out by LC with ultraviolet, fluorescent, or electrochemical detection or by LC‐ESI‐MS 42–44. However, considering that LC requires standard curves for quantitation and takes at least 10 min per sample, the combination of DIOS‐MS with the conventional SPE is comparatively rapid and will be applicable to the measurement of drug levels in blood.…”

Section: Resultsmentioning

confidence: 99%

Measurement of serum salicylate levels by solid‐phase extraction and desorption/ionization on silicon mass spectrometry

Okuno

Wada

2005

J. Mass Spectrom.

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The applicability of the matrix-free laser desorption/ionization on silicon mass spectrometry (DIOS-MS) to measuring serum drug levels was examined by analyzing serum salicylic acid. The optimized and simple solid-phase extraction (SPE) allowed good recovery, 88.9 +/- 5.8%, for 1.4 mM (200 mg/L) of salicylic acid in serum. The negative ion MS allowed measurements of deprotonated molecules without interference from other signals. Using a deuterium-labeled internal standard, good linearity was obtained in the 0.14 to 4.2 mM (20-600 mg/L) range, which was sufficient for monitoring the therapeutic anti-inflammatory dose. SPE followed by DIOS-MS is anticipated to be a method of measuring drug levels in blood and may allow high throughput analysis.

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“…This potential benefit might be mediated by the salicylate metabolite gentisic acid, which appears to have anti-oxidant properties (63). This metabolite can be measured in human plasma (64) and may also have beneficial effects by reducing the oxidation of low density lipoprotein (LDL) by glucose-derived radicals. In the aetiology of atherosclerosis, the pathological process associated with fatty plaques forming blood vessels, this oxidation appears to be important (65).…”

Section: Ii) Might Aspirin Be a 'Polypill' Against The Metabolic Syndmentioning

confidence: 99%

Aspirin Research And Reflections On The Welsh Contribution To 2010

2010

IJWH

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Aspirin reduces the risk of cardiovascular events and there is emerging evidence that it may also help prevent cancer. An increased use of the medicine could therefore have important public health benefits. In this two part paper, a summary of the current literature on aspirin is presented in part 1 and the contribution of Wales to aspirin research discussed in part 2. To help ensure that the benefits that aspirin can afford are maximised, perhaps an international scientific meeting might be a helpful next step.

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Simultaneous determination of gentisic, salicyluric and salicylic acid in human plasma using solid-phase extraction, liquid chromatography and electrospray ionization mass spectrometry

Cited by 12 publications

References 26 publications

Simultaneous quantitation of acetylsalicylic acid and clopidogrel along with their metabolites in human plasma using liquid chromatography tandem mass spectrometry

Simultaneous quantitation of acetylsalicylic acid and clopidogrel along with their metabolites in human plasma using liquid chromatography tandem mass spectrometry

Measurement of serum salicylate levels by solid‐phase extraction and desorption/ionization on silicon mass spectrometry

Aspirin Research And Reflections On The Welsh Contribution To 2010

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