Simultaneous determination of furosemide and spironolactone in pharmaceutical formulations by spectrophotometric method using principal component regression

Abstract

The combination of furosemide (furo) and spironolactone (spiro) is very effective in the treatment of heart failure. In that case, maintaining good quality of these drugs in commercial tablets is must. Therefore, a simple, economical, precise and accurate method, i.e; chemometric assisted UV spectroscopy, for simultaneous determination of furosemide and spironolactone in combined dosage form has been developed. In this study, principal component regression (PCR) has been reported for this purpose. A calibration set of 36 mixture solutions containing furosemide and spironolactone in methanol in the concentration range of 2.0-12.0 µg/ml and 5.0-30.0 µg/ml respectively has been prepared by means of an orthogonal experimental design. The absorbance data for the concentration set have been obtained by direct measurement in UV spectrophotometer at 101 wavelength points in the spectral region of 200-300 nm for the zero order spectra. The chemometric technique is also successfully applied to available pharmaceutical formulations, tablets, with no interference from excipients. The analytical performances of principal component regression are characterized by relative prediction errors and recoveries (%). The good recoveries obtained in this case proved that the proposed chemometric technique could be applied efficiently in the quality control of the studied drugs simultaneously in their mixture as well as in the commercial dosage form with satisfactory precision and accuracy as alternative analysis tools.

Smart Spectrophotometric Methods for Concurrent Determination of Furosemide and Spironolactone Mixture in Their Pharmaceutical Dosage Forms

“…Various methods were established for simultaneous analysis of FUR and SPR, including spectrophotometric methods (Millership, Parker, Donnelly, 2005;Israt et al, 2016;Chavan et al, 2018;Emam et al, 2018) and chromatographic methods (Maulik, Ketan, Shital, 2012;Ram, Dave, Joshi, 2012;Walash et al, 2012;Ram, Ram, Joshi, 2015;Naguib et al, 2018).…”

Development of methods for quantification of gelatin in vegetable capsule shell and for classification of capsule shell as from vegetable or animal sources by chemometric analysis of FTIR spectroscopic data

“…These methods need much time and capital intensive equipment with vast running cost, involve complex separation methods and require the use of different chemicals that are harmful to the environment. These drawbacks can be overcome by using multivariate analysis of spectroscopic data based chemometric methods which is less time consuming, limited use of chemical, straightforward and simple (Israt et al, 2016;Hassan, et al, 2018;Uddin et al, 2019). However, development of methods for quantification of gelatin in vegetable capsule shell and for classification of capsule shell as from vegetable or animal sources by chemometric analysis of FTIR spectroscopic data is a novel one through this study.…”

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“…It is also used to treat high blood pressure [3][4][5][6]. Several techniques and different analytical methods have been used for the furosemide determination such as spectrophotometric methods [7][8][9][10][11][12][13][14], flow injection [15,16], highperformance liquid chromatography methods [17-19], gas chromatography/mass spectrometry (GC/MS) [20, 21], electrochemical methods [22-24], potentiometric method [25], and atomic absorption spectroscopy [26]. The aim of this study is to develop method rapid, accurate, and simple for furosemide estimation with pyrogallol using charge transfer complex reaction, and also to apply the suggested method to several pharmaceutical formations.…”