Simultaneous determination of free mycophenolic acid and its glucuronide in serum of patients under mycophenolate mophetil therapy by ion-pair reversed-phase liquid chromatography with diode array UV detection

Relationship between MPA free fraction and free MPAG concentrations in heart transplant recipients based on simultaneous HPLC quantification of the target compounds in human plasma

“…The LOQ observed in the present method compares favourably with the HPLC-UV method (LOQ = 5 g/L) of Shipkova et al [13], the HPLCfluorescence method (LOQ = 5 g/L) of Shen et al [10]. For free MPAG, the limit of quantification was defined at 50 g/L, which is lower than the LOQ of previous reported HPLC-UV methods with values of 60 g/L [17], 900 g/L [8] and 2500 g/L [19].…”

“…The method exhibits a quantification limit of 5 g/L for MPA, with a sample volume of 100 L, which is lower than the previously published techniques [8,17] using ion-pair reversed-phase LC-UV method (26 g/L) and automated method (6 g/L) with an easier sample pretreatment procedure. The LOQ observed in the present method compares favourably with the HPLC-UV method (LOQ = 5 g/L) of Shipkova et al [13], the HPLCfluorescence method (LOQ = 5 g/L) of Shen et al [10].…”

“…Among the chromatographic methods described for the analysis of free MPA [8,10,[12][13][14]17], only two methods have been reported for the simultaneous determination of free MPA and free MPAG in a single run [8,17]. The first one, described by Aresta et al [17], has a quantification limit (LOQ) of 26 g/L for MPA and the second one, described by Mandla et al [8], has a recovery after dialysis estimated to 52%.…”

“…The first one, described by Aresta et al [17], has a quantification limit (LOQ) of 26 g/L for MPA and the second one, described by Mandla et al [8], has a recovery after dialysis estimated to 52%. However, free MPA determination requires more sensitive method to quantify very low concentrations, which could be found in plasma samples due to its high protein binding.…”

Development of a CE method for the determination of mycophenolic acid in human plasma: A comparison with HPLC

“…Up to date the procedures utilized for MPA drug monitoring mainly concern numerous HPLC methods, with [15][16][17][18][19] or without [11,20222] simultaneous MPAG measurement, EMIT immunoassays [23] and only one CE method [24].…”