Simultaneous determination of four components in Baige capsule by HPLC: application to pharmacokinetics and tissue distribution of normal and middle cerebral artery occlusion rats

Abstract

A sensitive and specific high-performance liquid chromatography (HPLC) method was developed and applied to the pharmacokinetics for simultaneous identification and quantification of four components - puerarin, daidzein, imperatorin and isoimperatorin - in the plasma and tissues of normal and middle cerebral artery occlusion rats after oral administration of Baige capsule. Ferulic was used as the internal standard. The extraction procedure was composed of two independent steps. The plasma was prepared by liquid-liquid extraction with light petroleum-acetic ether (1:1, v/v) first and then protein was precipitated with methanol. The tissue samples were weighted and homogenated with normal saline, then the homogenate was prepared by liquid-liquid extraction and protein precipitation. The parameters of pharmacokinetics were calculated using DAS 2.1.1 software. The calibration curves of all four components in the plasma and tissue homogenates were in good linearity in the measured range with R(2) ≥ 0.9921. The relative standard deviation of the intra- and inter-day accuracy at different levels was less than ±18.0%. In conclusion, the established method was a simple and effective one to simultaneously detect all four components in the plasma and tissues of rats, and was successfully applied in the pharmacokinetics of Baige capsule.

Preventive effects of imperatorin on perfluorohexanesulfonate-induced neuronal apoptosis via inhibition of intracellular calcium-mediated ERK pathway

“…A recent animal study also showed that imperatorin improved cognitive impairment induced by scopolamine [ 30 ]. Moreover, pharmacokinetic studies have shown that imperatorin is easily distributed in the brain after oral administration [ 31 32 ], suggesting its potential for neurological disease treatment. In the present study, we examined the protective effects of imperatorin on PFHxS-induced neuronal apoptosis and underlying mechanisms using rat CGC on PND 14.…”

A Novel Strategy of Electrochemically Treated ZrOCl2 Doped Carbon Paste Electrode for Sensitive Determination of Daidzein

“…Therefore, clinic determination of daidzein is necessary. Nowadays, some analytical methods are currently available for the determination of daidzein, such as HPLC 16–18, spectrophotometry 19, 20, GC‐MS 21 and high performance capillary electrophoresis 22, 23. Though these analytical techniques can achieve high sensitivity and good precision, they have to be operated by highly trained technicians, and require time‐consuming sample pretreatment, making them unsuitable for on‐site rapid monitoring.…”